The morphology and anatomy of ectomycorrhizas of Rhizopogon arctostaphyli, R. ellenae, R. flavofibrillosus, R.
occidentalis, R. rubescens, R. smithii, R. subcaerulescens and R. truncatus synthesized on Ponderosa pine (Pinus
ponderosa) in glasshouse conditions using spore slurries, are described and compared. All species produced a well-developed Hartig net, and a well-developed fungal mantle. The mantles of R. arctostaphyli, R. smithii and R.
subcaerulescens ectomycorrhizas were two-layered with outer mantle hyphae of wider diameter than inner mantle
hyphae. The mantle of R. subcaerulescens ectomycorrhizas also had distinctive peg-like structures (cystidia) along
peripheral hyphae. Rhizopogon truncatus ectomycorrhizas were tuberculate in morphology and had a rind-like
mantle enclosing adjacent roots. In addition, several species exhibited crystal inclusions in the outer mantle,
presumably at the interface between mantle and soil.