Jellyfishes have ecological and societal value, but our understanding of taxonomic identity of many jellyfish species remains limited. Here, an approach integrating morphological and molecular (16S ribosomal RNA and cytochrome oxidase I) data enables taxonomic assessment of the blubber jellyfish found in the Philippines. In this study, we aimed to resolve doubt on the taxonomy of Acromitoides purpurus, a valid binomen at the time of our research. Our morphological findings confirm that this jellyfish belongs to the genus Catostylus, and is distinct from known species of the genus inhabiting the Western Pacific, such as Catostylus ouwensi, Catostylus townsendi, and Catostylus mosaicus. Detailed morphological and molecular analyses of the type specimens from the Philippines with the other Catostylus species revive the binomen Catostylus purpurus and invalidate A. purpurus. Genetic analysis also distinguishes this Philippine jellyfish from C. townsendi and C. mosaicus. Through this study, we arranged several Catostylidae taxa into species inquirendae (Catostylus tripterus, Catostylus turgescens, and Acromitoides stiphropterus) and one genus inquirenda (Acromitoides) and provided an identification key for species of Catostylus. This comprehensive study confirms the blubber jellyfish as C. purpurus, enriching our understanding of jellyfish biodiversity. The integration of morphological and genetic analyses proves vital in resolving taxonomic ambiguities within the Catostylidae family and in the accurate identification of scyphozoan jellyfishes.

Replacing dietary animal protein with plant protein has a positive impact on greenhouse gas emissions(1) and preventing death from chronic disease(2). Despite being ideally situated to re-design menus, foodservices in hospitals have not focused on changing protein sources(3). Implementation in hospitals requires cooperation from stakeholders across the foodservice system e.g., managers, dietitians, menu planners, purchasers, cooks. A qualitative descriptive study design using semi-structured interviews explored perspectives of hospital foodservice stakeholders on increasing the proportion of plant to animal protein in hospital patient menus and outlined actions required to do this. Interviews were based on participatory backcasting with a “desirable future” defined as hospital patient menus containing, on average, more plant-based protein foods (PBPF) (i.e., legumes, nuts, plant-based meat alternatives) than animal-based protein foods (ABPF) (i.e., beef, lamb, pork, poultry, fish, eggs, dairy) by the year 2050. Analysis was completed using a general inductive approach. Thirty-five stakeholders participated (foodservice dietitians n = 10; foodservice managers, n = 6; dietetic professional leads n = 4; chef/cooks n = 4; information technology n = 4; manager [contracts] n = 4; manager [other] n = 3). Most (n = 25) supported patient menu changes to increase the proportion of plant to animal protein foods, though all described barriers. Common concerns were that patients wouldn’t eat the meals (n = 32), that menu re-design would have a negative impact on protein intake and malnutrition rates (n = 30), and that cost of PBPF was too high making the change unfeasible (n = 25). Benefits were an improvement in the nutrition profile of the menu and subsequent improvement to health (n = 16), lower cost of legumes compared to meat (n = 10), improvements in greenhouse gas emissions (n = 10) and opportunity to use the menu as an education tool (n = 8). We developed a model describing the required actions which began with a trigger for change followed by a cyclical design process, preparation, implementation and monitoring. The cyclical design process included stakeholder consultation, setting a target, choosing a strategy, developing a menu and recipes, finding product, planning the system and operation, and checking it worked. Participants valued gradual changes and maintaining choice during the change process. When prompted about specific strategies, stakeholders were most supportive of replacing ABPF with PBPFs in familiar recipes or replacing entire menu items (n = 21), adding PBPF options (n = 25), and moving PBPFs before ABPF-based items on the menu (n = 22). Hospital foodservices and policy makers wishing to increase the proportion of plant to animal protein in hospital patient menus can use the process and actions identified to plan pathways and communicate these to stakeholders. Future research should explore strategies for increasing the proportion of plant to animal protein while maintaining some ABPF on hospital menus, and evaluating effects of changes uptake, cost, estimated greenhouse gas emissions, satisfaction, dietary intake and health outcomes.

The Upper Permian sedimentary successions in the northern Sydney Basin have been the subject of several stratigraphic, sedimentological and coal petrographic studies, and recently, extensive U-Pb zircon dating has been carried out on tuffs in the Newcastle Coal Measures. However, detailed petrographic and geochemical studies of these successions are lacking. These are important because a major change in tectonic setting occurred prior to the Late Permian because of the Hunter-Bowen Orogeny that caused the uplift of the Carboniferous and Devonian successions in the Tamworth Group and Tablelands Complex adjacent to the Sydney Basin. This should be reflected in the detrital makeup of the Upper Permian rocks. This study provides data that confirms major changes did take place at this time. Petrographic analysis indicates that the source area is composed of sedimentary, felsic volcanic and plutonic and low-grade metamorphic rocks. Conglomerate clast composition analysis confirms these results, revealing a source region that is composed of felsic volcanics, cherts, mudstones and sandstones. Geochemical analysis suggests that the sediments are geochemically mature and have undergone a moderate degree of weathering. The provenance data presented in this paper indicate that the southern New England Orogen is the principal source of detritus in the basin. Discrimination diagrams confirm that the source rocks derive from an arc-related, contractional setting and agree with the provenance analyses that indicate sediment deposition in a retroarc foreland basin. This study offers new insights on the provenance and tectonic setting of the Northern Sydney Basin, eastern Australia.

OBJECTIVES/GOALS: This study examined patient perceptions on the benefits, barriers, and facilitators of conventional and complementary/behavioral pain strategies that can be offered in the ED setting including physical therapy, mindfulness, acupuncture, and yoga. METHODS/STUDY POPULATION: We conducted and recorded semi-structured interviews with 30 patients who presented to the ED with musculoskeletal pain. Interviews focused on patients’ perceptions of NP pain treatments, barriers/facilitators to utilization, and recommendations that would promote engagement. A hierarchical coding system was developed and refined using the interview guide, the Theory of Planned Behavior, and preliminary review of the transcripts. The iterative process of developing the coding system allowed us to identify preliminary themes. RESULTS/ANTICIPATED RESULTS: Patients believe education on pain and the mind-body connection would give a sense of pain control. Likely barriers to engaging at the ED include lights, noise, interruptions, and uncertainty of their medical status. Post-discharge NP treatment barriers are financial and logistical. Engagement can be facilitated by a desire to avoid opioids, familiarity with meditation practices, and consistent positive communication with the health care team. Patients desire evidence on effectiveness, including testimonials, and suggested NP techniques should be introduced early, with written materials used for post-discharge referral. Patients prefer in-person treatment but would appreciate a virtual option. The initial session should occur in-person to build trust and facilitate virtual session engagement. DISCUSSION/SIGNIFICANCE: Patients are willing to engage in nonpharmacological pain treatment, however the unpredictable ED environment, uncertainty of their medical status, and financial and time constraints are significant barriers.

Some physiological variables which could aid in assessing the welfare of beef cattle in feedlots were screened in this exploratory study. In two experiments, each of 42 days duration, the physiological responses of Bos taurus steers to three treatments were investigated: pasture (rotation between 1.5 hectare paddocks); a feedlot yard stocked at 12.0 m2 per head with a dry, firm pen surface; and a ‘high-density’ feedlot yard stocked at 6.0 m2 per head with a wet and muddy pen surface. Fourteen steers were used per group per experiment. Relative adrenal mass in both feedlot groups was 8-10% higher than in the pasture group, and this finding was supported by morphological measurements of the adrenal glands. Out of 17 immune variables examined, only serum IgA and the T-cell lymphocytes subpopulation WC+1 showed consistent differences between the feedlot and pasture groups. Interestingly, no differences were observed between the two feedlot treatments. It was concluded that although there may have been some disruption of epithelial/mucosal immunity, more support was required from other immune variables before it could be stated that the immune system was depressed and that pre-pathological states existed in the feedlot groups. However, measures of relative adrenal weight, adrenal index, serum IgA and WC1+ lymphocytes are good candidates for use in future welfare investigations of feedlot cattle.

The effect that breed standards and selective breeding practices have on the welfare of pedigree dogs has recently come under scrutiny from both the general public and scientific community. Recent research has suggested that breeding for particular aesthetic traits, such as tightly curled tails, highly domed skulls and short muzzles predisposes dogs with these traits to certain inherited defects, such as spina bifida, syringomyelia and brachycephalic airway obstruction syndrome, respectively. Further to this, there is a very large number of inherited diseases that are not related to breed standards, which are thought to be prevalent, partly as a consequence of inbreeding and restricted breeding pools. Inherited diseases, whether linked to conformation or not, have varying impact on the individuals affected by them, and affect varying proportions of the pedigree dog population. Some diseases affect few breeds but are highly prevalent in predisposed breeds. Other diseases affect many breeds, but have low prevalence within each breed. In this paper, we discuss the use of risk analysis and severity diagrams as means of mapping the overall problem of inherited disorders in pedigree dogs and, more specifically, the welfare impact of specific diseases in particular breeds.

All emergency and casualty bovines in the Republic of Ireland that are deemed to be fit for human consumption must be accompanied to the slaughterhouse by an official veterinary certificate (VC). Cullinane et al (2010) conducted a review of bovine cases consigned under veterinary certification to emergency (ES) and casualty (CS) slaughter in the Republic of Ireland during 2006 to 2008. The current paper further evaluates these results, with emphasis on the period of validity, transport distance and transport conditions, and considers whether the current VC adequately protects welfare. Of 1,255 veterinary certificates, the median time between certification and slaughter was 1 (minimum 0, maximum 452) day, with one-fifth in excess of three days. The median straight-line distance between farm and slaughterhouse was 22 km; 82 and 98% study animals were transported distances of no more than 40 and 100 km, respectively. In 27% of VCs, no slaughterhouse was designated; hence the transport distance was not limited. In 77% of all cases, the disability/injury was related to the locomotor system, including 35% with limb fractures. Veterinary certification of the latter as being fit for transport appears to have been in contravention of animal transport and welfare legislation. The welfare of animals would be improved if each VC included a certified period of validity. In future, veterinary certificates should state clearly a maximum period of validity between certification and slaughter, a maximum distance that the animal should be transported and a minimum level of comfort under which the animal must be transported. Historically, in the Republic of Ireland, there was no suitable non-transport alternative available to cattle producers wanting to salvage an otherwise healthy animal that had suffered an accident or injury resulting in acute pain. An alternative is now available, with the introduction of an on-farm emergency slaughter policy.

Coronavirus disease 2019 (COVID-19) vaccination rates of a large health system reflected their respective service areas but varied by work role. Nurse vaccination rates were higher (56.9%) and rates among nursing support personnel were lower (38.6%) than those of their communities (51.7%; P < .001). Physician vaccination rates were highest (71.6%) and were not associated with community vaccination levels.

The role of the Eurasian badger (Meles meles) as a wildlife host has complicated the management of bovine tuberculosis (bTB) in cattle. Badger ranging behaviour has previously been found to be altered by culling of badgers and has been suggested to increase the transmission of bTB either among badgers or between badgers and cattle. In 2014, a five-year bTB intervention research project in a 100 km2 area in Northern Ireland was initiated involving selective removal of dual path platform (DPP) VetTB (immunoassay) test positive badgers and vaccination followed by release of DPP test negative badgers (‘Test and Vaccinate or Remove’). Home range sizes, based on position data obtained from global positioning system collared badgers, were compared between the first year of the project, where no DPP test positive badgers were removed, and follow-up years 2–4 when DPP test positive badgers were removed. A total of 105 individual badgers were followed over 21 200 collar tracking nights. Using multivariable analyses, neither annual nor monthly home ranges differed significantly in size between years, suggesting they were not significantly altered by the bTB intervention that was applied in the study area.

The first demonstration of laser action in ruby was made in 1960 by T. H. Maiman of Hughes Research Laboratories, USA. Many laboratories worldwide began the search for lasers using different materials, operating at different wavelengths. In the UK, academia, industry and the central laboratories took up the challenge from the earliest days to develop these systems for a broad range of applications. This historical review looks at the contribution the UK has made to the advancement of the technology, the development of systems and components and their exploitation over the last 60 years.

Infants born preterm miss out on the peak period of in utero DHA accretion to the brain during the last trimester of pregnancy which is hypothesised to contribute to the increased prevalence of neurodevelopmental deficits in this population. This study aimed to determine whether DHA supplementation in infants born preterm improves attention at 18 months’ corrected age. This is a follow-up of a subset of infants who participated in the N3RO randomised controlled trial. Infants were randomised to receive an enteral emulsion of high-dose DHA (60 mg/kg per d) or no DHA (soya oil – control) from within the first days of birth until 36 weeks’ post-menstrual age. The assessment of attention involved three tasks requiring the child to maintain attention on toy/s in either the presence or absence of competition or a distractor. The primary outcome was the child’s latency of distractibility when attention was focused on a toy. The primary outcome was available for seventy-three of the 120 infants that were eligible to participate. There was no evidence of a difference between groups in the latency of distractibility (adjusted mean difference: 0·08 s, 95 % CI –0·81, 0·97; P = 0·86). Enteral DHA supplementation did not result in improved attention in infants born preterm at 18 months’ corrected age.