To send content items to your account,
please confirm that you agree to abide by our usage policies.
If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account.
Find out more about sending content to .
To send content items to your Kindle, first ensure firstname.lastname@example.org
is added to your Approved Personal Document E-mail List under your Personal Document Settings
on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part
of your Kindle email address below.
Find out more about sending to your Kindle.
Note you can select to send to either the @free.kindle.com or @kindle.com variations.
‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi.
‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
Cryopreservation protocols for isolated oocytes and complex ovarian tissues can be broadly classified as equilibrium freezing (slow-freezing protocols) or rapid freezing (vitrification protocols). Oocyte cryopreservation requires that the gametes tolerate three non-physiological conditions: exposure to molar concentrations of cryoprotective agents (CPAs); cooling to subzero temperatures and removal of or conversion of almost all of the liquid cell water into the solid state. Penetrating CPAs, such as glycerol, dimethyl sulphoxide (DMSO), ethylene glycol (EG) and 1,2-propanediol (PrOH), are all membrane-soluble and can pass into cells. Non-penetrating CPAs include sucrose, glucose, trehalose and polymers such as hydroxyethyl starch and polyvinyl pyrrolidone. The rate at which cells are cooled is fundamental to the success of cryopreservation. Consequently, at the time of writing, mature metaphase II (MII) oocyte cryopreservation remains a potential solution rather than a practical remedy for infertile women.
Email your librarian or administrator to recommend adding this to your organisation's collection.