The Czech language has two trills produced with the anterior tongue, represented in the Czech orthography as /r/ and /ř/. The phonetic characterization of the latter trill, currently transcribed in the IPA as [
], has been especially controversial. The present study uses ultrasound, electroglottography (EGG) and acoustic evidence to examine these trills, with the particular aim of achieving a more phonetically grounded representation of /ř/. In the first part of the paper, ultrasound imaging is used to compare tongue positions between /r/ and /ř/, ruling out tongue height as the key factor distinguishing the two sounds. The second part uses EGG and acoustic evidence to examine phonatory aspects of production. Results implicate vocal fold abduction as the more robust factor distinguishing /r/ from /ř/. Consequently, we posit [
] as a more appropriate phonetic characterization of /ř/.