Bismuth has been used as an antimicrobial agent for treating gastrointestinal disorders, and has been used in the eradication of Helicobacter pylori. The aim of the present study was to analyze the antimicrobial activity of different Bi compounds against opportunistic pathogens. Ten bismuth compounds were tested with three different concentrations (60, 30 and 10%), against pure cultures of the following bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis. The results show that Bismuth subsalicylate, Bismuth trioxide and Bismuth subgallate had a good antibacterial activity however, Bismuth subsalicylate was the most effective in the inhibition of the four bacterial strains tested. In this study we confirm the antibacterial properties of Bi-based compounds for other bacteria than H. pylori.