This cross-sectional study was undertaken to assess the status of school-based water, sanitation and hygiene (WASH) resources and soil-transmitted helminthiasis (STH) in Odeda, Ogun State, Nigeria. The status and condition of WASH resources were determined in eight selected schools (three with WASH support and five without). Stool samples were also collected from 428 pupils from both school categories and screened for STH infections. Findings showed that water and sanitation conditions of WASH resource programming schools were better than those in schools without WASH support. However, pupils’ knowledge about STH infections and basic hygiene were not significantly different between both categories of school (P > 0·05). The prevalence of Hookworm, Ascariasis and Trichuriasis cases recorded were 26·2, 18·2 and 1·6% respectively, with an overall prevalence of 33·4%. By school categories, Ascariasis (14·9 vs 20·6), Hookworm (17·7 vs 32·0), Trichuriasis (1·7 vs 1·6) and, any STH prevalence (27·4 vs 37·5) were recorded for schools with WASH resource programming and those without respectively. A greater portion of the infected children 95 (66·4%) were from schools without WASH support. Our findings imply that WASH resource may have very positive impact on the control of STH among school children. However, there is need for improvement on coverage, adequacy and maintenance of WASH facilities in school.