Accurate placement of biomaterials at nanoscale resolution opens new capabilities for biological sensing, cell manipulation, and control of cellular transduction cascades. We demonstrate that lipid molecules can be patterned on silicon using a polymer lift-off technique. Patterned lipid bilayers serve as biomaterial patterning platforms useful for studies of cellular function. Submicron feature sizes were achieved using this templating technique which is suitable for delicate biomaterials1. Projection lithography and reactive ion etching were used to pattern a Parylene-coated surface. The patterned surface was subsequently exposed to 100 nm unilamellar lipid vesicles that bound to the native oxide surfaces of silicon and spread to form supported lipid bilayers. The nanoscale pattern is realized as the polymer is peeled away in deionized water. The versatility of this method is demonstrated by the successful preparation of functionalized lipid bilayer surfaces. Specific intermolecular interactions were demonstrated between supported membranes: DPPE-PE (2000) biotin/avidin, dinitrophenol (DNP)-conjugated lipids/anti-DNP IgE, and cationic lipid and M13 dsDNA:YOYO-1.