Discontinuity between suitable aquatic and terrestrial habitats forces many amphibian species with aquatic larvae to undertake risky breeding migrations through disturbed environments, potentially contributing to population declines by reducing survival of adults and juveniles (Becker et al. 2007). The north-western region of the state of São Paulo, Brazil, is characterized by fragments of mesophytic semideciduous forest and savanna, surrounded by a matrix of pasture, plantations and urban areas with only 4% of the remaining original vegetation of the state (Ribeiro et al. 2009). Currently, 36 anuran species have been recorded in this region, corresponding to almost 15% of the state's species (Araújo et al. 2009). The anuran species of this region are associated with open environments or are widely distributed in the different Brazilian biomes (Duellman 1999). Silva & Rossa-Feres (2007) showed the importance of forest fragments in this region for 12 anuran species that breed in pools located in a pasture matrix.