Aquafeeds for carnivorous species face a nutritional-technological conundrum: containing sufficient starch to meet specific manufacturing requirements for binding, extrusion, and expansion, but ideally containing as little starch as possible owing to their limited ability to utilise carbohydrates. The present study evaluated the effects of dietary starch with different amylose to amylopectin ratios and resistant starch contents on growth performance, hepatic glycogen accumulation, and glucose metabolism of an important cultured carnivorous finfish, largemouth bass (Micropterus salmoides). A common starch source (α-cassava starch) was tested as is or after being enzymatically de-branched at three different inclusion levels in diets for largemouth bass. Results showed that the increased dietary starch levels compromised performance and high dietary α-cassava starch content led to obvious liver damage. However, the growth performances of fish fed the diets with de-branched starch were improved, and no manifest liver damages were observed even at the higher inclusion level. The increasing dietary starch contents significant increased hepatic glycogen accumulation, but not when de-branched starch was used. High dietary starch content, without regard to starch sources, had no effect on the expression of glucose metabolism related genes, except for downregulation of insulin receptor expression. However, the use of dietary de-branched starch promoted the expression of genes involved in insulin pathway and glycolysis. In conclusion, this study showed that the use of starch sources with a high amylose to amylopectin ratio and resistant starch in the feed for cultured carnivorous finfish could alleviate the hepatic glycogen deposition through regulating insulin pathway and glycolysis.