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Biofilm Formation of Staphylococcus epidermidis With and Without Collagen Imaged Using Atmospheric Scanning Electron Microscopy and Antibacterial Effect of Ag-decorated Polymeric Particles Imaged by Transmission Electron Microscopy
Chisato Takahashi, Keiichi Moriguchi, Miki Hori, Tatsushi Kawai, Mari Sato, Chikara Sato
Journal: Microscopy and Microanalysis / Volume 28 / Issue S1 / August 2022
Published online by Cambridge University Press: 22 July 2022, pp. 1432-1433
Print publication: August 2022
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Correlative Light-Electron Microscopy of Neurons and Brains in Liquid
Chikara Sato, Takaaki Kinoshita, Takeshi Uemura, Naoki Kasahata, Mari Sato, Shoko Nishihara, Masami Naya
Journal: Microscopy and Microanalysis / Volume 27 / Issue S2 / November 2021
Published online by Cambridge University Press: 03 December 2021, pp. 5-6
Print publication: November 2021
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Liquid-phase imaging of bone development and calcification by atmospheric scanning electron microscopy (ASEM): Application to immuno-labeling and rapid tissue observation of genetically modified mouse
Eiko Sakai, Mari Sato, Nassirhadjy Memtily, Takayuki Tsukuba, Chikara Sato
Journal: Microscopy and Microanalysis / Volume 27 / Issue S1 / August 2021
Published online by Cambridge University Press: 30 July 2021, pp. 2288-2289
Print publication: August 2021
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Observation of Bone Tissue Metabolism and Bacterial Biofilm in Aqueous Solution Using ASEM
Chikara Sato, Masami Naya, Memtily Nassirhadjy, Mari Sato, Naoki Kasahata, Shinya Sugimoto
Journal: Microscopy and Microanalysis / Volume 26 / Issue S2 / August 2020
Published online by Cambridge University Press: 30 July 2020, pp. 1340-1341
Print publication: August 2020
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CLEM of Neurons, Tissues and Biofilms immersed in Liquid using The Atmospheric Scanning Electron Microscope (ASEM): Dual Gold-Labeling
Chikara Sato, Nassir Memtily, Mari Sato, Toshiko Yamazawa, Takaaki Kinoshita, Shoko Nishihara, Shinya Sugimoto
Journal: Microscopy and Microanalysis / Volume 24 / Issue S1 / August 2018
Published online by Cambridge University Press: 01 August 2018, pp. 340-341
Print publication: August 2018
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The Atmospheric Scanning Electron Microscope (ASEM) observes the axonal compartmentalization and microtubule formation in neurons.
Takaaki Kinoshita, Chikara Sato, Shoko Nishihara
Journal: Microscopy and Microanalysis / Volume 23 / Issue S1 / July 2017
Published online by Cambridge University Press: 04 August 2017, pp. 1298-1299
Print publication: July 2017
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5 - Observations in Liquids Using an Inverted SEM
from Part I - Technique
- By Chikara Sato, Mitsuo Suga
Edited by Frances M. Ross, IBM T. J. Watson Research Center, New York
Book: Liquid Cell Electron Microscopy
Published online: 22 December 2016
Print publication: 24 November 2016, pp 106-126
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The Atmospheric Scanning Electron Microscope (ASEM) observes the Cultured Fluorescent Neuron
Tatsuhiko Ebihara, Hidetoshi Nishiyama, Mitsuo Suga, Chikara Sato
Journal: Microscopy and Microanalysis / Volume 21 / Issue S3 / August 2015
Published online by Cambridge University Press: 23 September 2015, pp. 889-890
Print publication: August 2015
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Quick Observation of Tissues in Solution by Atmospheric Scanning Electron Microscopy (ASEM)
Nassirhadjy Memtily, Mari Sato, Tomoko Okada, Tatsuhiko Ebihara, Kazuhiro Mio, Mitsuo Suga, Hiditoshi Nishiyama, Chikara Sato
Journal: Microscopy and Microanalysis / Volume 21 / Issue S3 / August 2015
Published online by Cambridge University Press: 23 September 2015, pp. 399-400
Print publication: August 2015
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Wide Area Observation of Fully Hydrophilic Tissue Achieved by Sliding It on the Dish of the Atmospheric Scanning Electron Microscope (ASEM)
Chikara Sato, Mari Sato, Tatsuhiko Ebihara, Hiditoshi Nishiyama, Mitsuo Suga, Nassirhadjy Memtily
Journal: Microscopy and Microanalysis / Volume 21 / Issue S3 / August 2015
Published online by Cambridge University Press: 23 September 2015, pp. 1503-1504
Print publication: August 2015
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Observation of Tissues in Solution by Atmospheric Scanning Electron Microscope (ASEM)
Nassirhadjy Memtily, Kazuhiro Mio, Mari Sato, Tatsuhiko Ebihara, Chikara Sato
Journal: Microscopy and Microanalysis / Volume 20 / Issue S3 / August 2014
Published online by Cambridge University Press: 27 August 2014, pp. 978-979
Print publication: August 2014
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The Atmospheric Scanning Electron Microscope (ASEM) Observes Axonal Segmentation and Synaptic Induction in Solution
Chikara Sato, Takaaki Kinoshita, Takeshi Uemura, Kazumi Hiarno, Masaaki Kawata, Hidetoshi Nishiyama, Mari Sato, Tatsuhiko Ebihara, Mitsuo Suga, Shoko Nishihara
Journal: Microscopy and Microanalysis / Volume 20 / Issue S3 / August 2014
Published online by Cambridge University Press: 27 August 2014, pp. 972-973
Print publication: August 2014
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Immuno-Electron Microscopy of Primary Cell Cultures from Genetically Modified Animals in Liquid by Atmospheric Scanning Electron Microscopy
Takaaki Kinoshita, Yosio Mori, Kazumi Hirano, Shinya Sugimoto, Ken-ichi Okuda, Shunsuke Matsumoto, Takeshi Namiki, Tatsuhiko Ebihara, Masaaki Kawata, Hidetoshi Nishiyama, Mari Sato, Mitsuo Suga, Kenichi Higashiyama, Kenji Sonomoto, Yoshimitsu Mizunoe, Shoko Nishihara, Chikara Sato
Journal: Microscopy and Microanalysis / Volume 20 / Issue 2 / April 2014
Published online by Cambridge University Press: 25 February 2014, pp. 469-483
Print publication: April 2014
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View abstract

High-throughput immuno-electron microscopy is required to capture the protein–protein interactions realizing physiological functions. Atmospheric scanning electron microscopy (ASEM) allows in situ correlative light and electron microscopy of samples in liquid in an open atmospheric environment. Cells are cultured in a few milliliters of medium directly in the ASEM dish, which can be coated and transferred to an incubator as required. Here, cells were imaged by optical or fluorescence microscopy, and at high resolution by gold-labeled immuno-ASEM, sometimes with additional metal staining. Axonal partitioning of neurons was correlated with specific cytoskeletal structures, including microtubules, using primary-culture neurons from wild type Drosophila, and the involvement of ankyrin in the formation of the intra-axonal segmentation boundary was studied using neurons from an ankyrin-deficient mutant. Rubella virus replication producing anti-double-stranded RNA was captured at the host cell’s plasma membrane. Fas receptosome formation was associated with clathrin internalization near the surface of primitive endoderm cells. Positively charged Nanogold clearly revealed the cell outlines of primitive endoderm cells, and the cell division of lactic acid bacteria. Based on these experiments, ASEM promises to allow the study of protein interactions in various complexes in a natural environment of aqueous liquid in the near future.

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Biofilm Formation of Staphylococcus epidermidis With and Without Collagen Imaged Using Atmospheric Scanning Electron Microscopy and Antibacterial Effect of Ag-decorated Polymeric Particles Imaged by Transmission Electron Microscopy

Correlative Light-Electron Microscopy of Neurons and Brains in Liquid

Liquid-phase imaging of bone development and calcification by atmospheric scanning electron microscopy (ASEM): Application to immuno-labeling and rapid tissue observation of genetically modified mouse

Observation of Bone Tissue Metabolism and Bacterial Biofilm in Aqueous Solution Using ASEM

CLEM of Neurons, Tissues and Biofilms immersed in Liquid using The Atmospheric Scanning Electron Microscope (ASEM): Dual Gold-Labeling

The Atmospheric Scanning Electron Microscope (ASEM) observes the axonal compartmentalization and microtubule formation in neurons.

5 - Observations in Liquids Using an Inverted SEM

The Atmospheric Scanning Electron Microscope (ASEM) observes the Cultured Fluorescent Neuron

Quick Observation of Tissues in Solution by Atmospheric Scanning Electron Microscopy (ASEM)

Wide Area Observation of Fully Hydrophilic Tissue Achieved by Sliding It on the Dish of the Atmospheric Scanning Electron Microscope (ASEM)

Observation of Tissues in Solution by Atmospheric Scanning Electron Microscope (ASEM)

The Atmospheric Scanning Electron Microscope (ASEM) Observes Axonal Segmentation and Synaptic Induction in Solution

Immuno-Electron Microscopy of Primary Cell Cultures from Genetically Modified Animals in Liquid by Atmospheric Scanning Electron Microscopy

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13 results