The alvinocaridid shrimp Shinkaicaris leurokolos Kikuchi and Hashimoto, 2000, is an evolutionarily important deep-sea species in hydrothermal vents of north-western Pacific. A genome survey of S. leurokolos was carried out in order to provide a foundation for its whole-genome sequencing. A total of 599 Gb high-quality sequence data were obtained in the study, representing approximately 118× coverage of the S. leurokolos genome. According to the 17-mer distribution frequency, the estimated genome size was 5.08 Gb, and its heterozygosity ratio and percentage of repeated sequences were 2.85 and 87.03%, respectively, showing a complex genome. The final scaffold assembly accounted for a total size of 9.53 Gb (32,796,062 scaffolds, N50 = 597 bp). Repetitive elements nearly constituted 45% of the nuclear genome, among which the most ubiquitous were long interspersed nuclear elements, DNA transposons and long-terminal repeat elements. A total of 12,121,553 genomic simple sequence repeats were identified, with the most frequent repeat motif being di-nucleotide (70.27%), followed by tri-nucleotide and tetra-nucleotide. From the genome survey sequences, the mitochondrial genome of S. leurokolos was also constructed and 71 single nucleotide polymorphisms were identified by comparison with previous published reference. This is the first report of de novo whole-genome sequencing and assembly of S. leurokolos. These newly developed genomic data contribute to a better understanding of genomic characteristics of shrimps from deep-sea chemosynthetic ecosystems, and provides valuable resources for further molecular marker development.

For close to four decades, Claudia Bucher’s “scientart” has created myriad experiential habitats for her performing body, while inviting others to join her imaginary leaps. From personifying an outer-space lichen colony to meditating midair as an “anemochore kite,” Bucher demonstrates empathic kinships with other beings and nonbeings, calling forth alternative eco-systems in which sentience thrives as the common denominator for all inhabitants.

A walking robot consisting of double Stewarts parallel legs was designed by our research team in the past time, which was mainly used for the transportation of the wounded after the disaster. In order to promote stability of control locomotion and ensure invariably horizontal state of the robot platform in the process of motion, the central pattern generator (CPG) based on particle swarm optimization (PSO) is presented to optimize the kinematic model. The purpose of optimization is to solve the hysteresis problem of displacement variation among the electric cylinders. Moreover, the dynamic model of the robot is established, which can provide mechanical basis for the feedback of control signal and make the robot move stably. The simulation results show that the displacement hysteresis problem of the electric cylinders is solved well. Meanwhile, compared with simulation results based on GA-CPG method, it is demonstrated that the robot motion planned using PSO-CPG method has better motion stability and can avoid the impact of legs landing during the transition phase of the motion cycle. The experimental results show that the platform on the robot can maintain an invariably horizontal state, and the locomotion is more stable. It verifies the feasibility of PSO-CPG model and the correctness of the dynamic model of the parallel mobile rescue robot.

Turbulent plane-Couette flow suspended with finite-size spheroidal particles is studied using fully particle-resolved direct numerical simulations. The effects of particle aspect ratio on turbulent arguments and particle statistics are explored, leading to the same conclusions as the previous experimental findings (Wang et al., J. Fluid Mech., vol. 937, 2022, A15). By performing stress analysis, we find that the presence of particles introduces extra stresses to the system and accounts for the global drag increases. The particle-laden flow cases exhibit spectra that are consistent with the scalings $k^{-5/3}$ and $k^{-3}$ in the large and small scales, respectively. While the $k^{-3}$ scaling observed in the particle-laden flow is reminiscent of bubbly flow, an examination of the particle Reynolds number suggests that the mechanism responsible may not be attributable to the pseudo-turbulence induced by particles as in the case of bubbles. In the view of particle statistics, we observe that spherical and non-spherical particles cluster preferentially in the near-wall and the bulk region, respectively, and that the orientations of non-spherical particles are affected by their aspect ratios, especially in the near-wall region. The present numerical results, combined with previous experimental findings in Wang et al. (J. Fluid Mech., vol. 937, 2022, A15), provide in-depth information on both the fluid and the particle phase, contributing to a better understanding of particle suspension in shear flows.

The benefits of branched-chain amino acid (BCAA) administration after hepatic intervention in patients with liver diseases remain unclear. We conducted a systematic review and meta-analysis to evaluate the effects of BCAA on patients undergoing hepatectomy, trans-arterial embolisation and radiofrequency ablation. Relevant randomised controlled trials (RCT) were obtained from PubMed, EMBASE and Cochrane Library databases. A meta-analysis was performed to calculate the pooled effect size by using random-effects models. The primary outcomes were survival and tumour recurrence. The secondary outcomes were hospital stay, nutrition status, biochemistry profile, complication rate of liver treatment and adverse effect of BCAA supplementation. In total, eleven RCT involving 750 patients were included. Our meta-analysis showed no significant difference in the rates of tumour recurrence and overall survival between the BCAA and control groups. However, the pooled estimate showed that BCAA supplementation in patients undergoing hepatic intervention significantly increased serum albumin (mean difference (MD): 0·11 g/dl, 95 % CI: 0·02, 0·20; 5 RCT) at 6 months and cholinesterase level (MD: 50·00 U/L, 95 % CI: 21·08, 78·92; 1 RCT) at 12 months and reduced ascites incidence (risk ratio: 0·39, 95 % CI: 0·21, 0·71; 4 RCT) at 12 months compared with the control group. Additionally, BCAA administration significantly increased body weight at 6 months and 12 months and increased arm circumference at 12 months. In conclusion, BCAA supplementation significantly improved the liver function, reduced the incidence of ascites and increased body weight and arm circumference. Thus, BCAA supplementation may beneficial for selected patients undergoing liver intervention.

Many Indigenous groups in Taiwan, including the Bunun, inhabited remote mountainous regions. Beginning in the 1930s, all mountain settlements were relocated to lower-lying areas by the colonial authorities. These groups lost the territories where they used to hunt, practice slash-and-burn agriculture, and carry out other social and cultural practices. Having being separated from their ancestral lands for decades, their knowledge of their former settlements and traditional ways of life is gradually disappearing. In recent years, there is a tendency among the younger generation of Indigenous people to organize and participate in roots-seeking expeditions. As their knowledge about the former settlements is limited, they seek help from the elderly—and archaeologists. Since 2014, I have collaborated with Bunun communities, recording their ancestors’ lands in the Lakulaku River Basin by joining archaeological surveys on roots-seeking trips. During these surveys, I had to learn Bunun values and gain knowledge of the Lakulaku River Basin via the bodily experience of moving through and being in the landscape with its traditional inhabitants. By applying remote-sensing technologies such as airborne lidar in our surveys, our team managed to identify and record settlements that were unfamiliar to the Bunun.

Given a Borel probability measure µ on $\mathbb{R}^n$ and a real matrix $R\in M_n(\mathbb{R})$. We call R a spectral eigenmatrix of the measure µ if there exists a countable set $\Lambda\subset \mathbb{R}^n$ such that the sets $E_\Lambda=\big\{{\rm e}^{2\pi i \langle\lambda,x\rangle}:\lambda\in \Lambda\big\}$ and $E_{R\Lambda}=\big\{{\rm e}^{2\pi i \langle R\lambda,x\rangle}:\lambda\in \Lambda\big\}$ are both orthonormal bases for the Hilbert space $L^2(\mu)$. In this paper, we study the structure of spectral eigenmatrix of the planar self-affine measure $\mu_{M,D}$ generated by an expanding integer matrix $M\in M_2(2\mathbb{Z})$ and the four-elements digit set $D = \{(0,0)^t,(1,0)^t,(0,1)^t,(-1,-1)^t\}$. Some sufficient and/or necessary conditions for R to be a spectral eigenmatrix of $\mu_{M,D}$ are given.

As there exists a growing demand for chronological research and tracer applications using radiocarbon (14C) analyses of samples smaller than 100 μg C, a compact micro-specific hydrogen graphitization method has been developed at the Xi’an Accelerator Mass Spectrometry (AMS) Center. This article describes the performance of the system and the mass of carbon background produced during ultra-small sample preparation. Furthermore, we discuss the results of contamination corrections and perform 14C analyses on small samples with known age or reference values. The results reveal that our 14C analysis of ultra-small samples of 10–100 μg C can obtain accurate and reliable results, and the micro-scale 14C-AMS analysis technique meets our research objectives for dating and tracer applications.

A steady granular flow experiment was performed in a confined annular shear cell to examine how the wall friction coefficient $\mu _w$ degrades from the intrinsic sliding friction coefficient $f$ between the grains and the container wall. Two existing models are invoked to examine the decay trend of $\mu _w/f$ in view of the ratio of shear velocity to the square root of granular temperature $\chi$ (Artoni & Richard, Phys. Rev. Lett., vol. 115, 2015, 158001) and the ratio of grain angular and slip velocities $\varOmega$ (Yang & Huang, Granul. Matt., vol. 18, issue 4, 2016, p. 77), respectively. As both models correlate $\mu _w/f$ to different flow properties, a hidden relation is speculated between $\chi$ and $\varOmega$, or equivalently, between the granular temperature and the grain rotation speed. We used experiment data to confirm and reveal this hidden relation. From there, a unified $\mu _w/f-\chi$ model is proposed with physical meanings for the model coefficients and to show general agreement with the measured trend. Hence we may conclude that both the fluctuations in grain translations and their mean rotation are the crucial yet equivalent mechanisms to degrade $\mu _w/f$.

SARS-CoV-2 rapidly spreads among humans via social networks, with social mixing and network characteristics potentially facilitating transmission. However, limited data on topological structural features has hindered in-depth studies. Existing research is based on snapshot analyses, preventing temporal investigations of network changes. Comparing network characteristics over time offers additional insights into transmission dynamics. We examined confirmed COVID-19 patients from an eastern Chinese province, analyzing social mixing and network characteristics using transmission network topology before and after widespread interventions. Between the two time periods, the percentage of singleton networks increased from 38.9$ \% $ to 62.8$ \% $$ (p<0.001) $; the average shortest path length decreased from 1.53 to 1.14 $ (p<0.001) $; the average betweenness reduced from 0.65 to 0.11$ (p<0.001) $; the average cluster size dropped from 4.05 to 2.72 $ (p=0.004) $; and the out-degree had a slight but nonsignificant decline from 0.75 to 0.63 $ (p=0.099). $ Results show that nonpharmaceutical interventions effectively disrupted transmission networks, preventing further disease spread. Additionally, we found that the networks’ dynamic structure provided more information than solely examining infection curves after applying descriptive and agent-based modeling approaches. In summary, we investigated social mixing and network characteristics of COVID-19 patients during different pandemic stages, revealing transmission network heterogeneities.

This paper studies the synchronization control of the blanket remote maintenance robot (BRMR) of the China fusion engineering test reactor (CFETR). First, the general state space mathematical model of BRMR was established by using a physical-based method. Second, based on the receding horizon optimization of model predictive control (MPC) and cross-coupling error reduction in cross-coupling control (CCC), the innovative MPC-CCC controller was proposed to realize the single-system and multisystem error convergence and high accuracy transportation of blanket through the high accuracy synchronization control of BRMR. Third, to verify the control effectiveness of the MPC-CCC controller, two types of simulations and experiments were implied compared with the original proportional-integral (PI) controller in Mover. Results showed that simulation and experiments were highly consistent. It is found that the use of an MPC-CCC controller can result in up to a 70% reduction in displacement error and up to a 59% reduction in synchronization error compared to the PI controller. And the accuracy of the MPC-CCC controller satisfies the real requirement of the maintenance process of the blanket. This work provides the theoretical basis and practical experience for the highly stable, safe, and efficient maintenance of blankets in the future.

The present study investigated the differential effects of pitched and unpitched musicianship on tone identification and word learning. We recruited 44 Cantonese-pitched musicians, unpitched musicians, and non-musicians. They completed a Thai tone identification task and seven sessions of Thai tone word learning. In the tone identification task, the pitched musicians outperformed the non-musicians but the unpitched musicians did not. In session 1 of the tone word learning task, the three groups showed similar accuracies. In session 7, the pitched musicians outperformed the non-musicians but the unpitched musicians did not. The results indicate that the musical advantage in tone identification and word learning hinges on pitched musicianship. From a theoretical perspective, these findings support the precision element of the OPERA hypothesis. Broadly, they reflect the need to consider the heterogeneity of musicianship when studying music-to-language transfer. Practically, the findings highlight the potential of pitched music training in enhancing tone word learning proficiency. Furthermore, the choice of musical instrument may matter to music-to-language transfer.

In zebrafish, a punctate band of F-actin is reported to develop in the external yolk syncytial layer (E-YSL) during the latter part of epiboly in zebrafish embryos. Here, electron microscopy (EM) and fluorescence confocal microscopy were conducted to investigate dynamic changes in the E-YSL membrane during epiboly. Using scanning EM, we report that the surface of the E-YSL is highly convoluted, consisting of a complex interwoven network of branching membrane surface microvilli-like protrusions. The region of membrane surface protrusions was relatively wide at 30% epiboly but narrowed as epiboly progressed. This narrowing was coincident with the formation of the punctate actin band. We also demonstrated using immunogold transmission EM that actin clusters were localized at the membrane surface mainly within the protrusions as well as in deeper locations of the E-YSL. Furthermore, during the latter part of epiboly, the punctate actin band was coincident with a region of highly dynamic endocytosis. Treatment with cytochalasin B led to the disruption of the punctate actin band and the membrane surface protrusions, as well as the attenuation of endocytosis. Together, our data suggest that, in the E-YSL, the region encompassing the membrane surface protrusions and its associated punctate actin band are likely to be an integral part of the localized endocytosis, which is important for the progression of epiboly in zebrafish embryos.