Sedimentological, faunal, and archaeological investigations at the Sunshine Locality, Long Valley, Nevada reveal a history of human adaptation and environmental change at the last glacial–interglacial transition in North America's north-central Great Basin. The locality contains a suite of lacustrine, alluvial, and eolian deposits associated with fluvially reworked faunal remains and Paleoindian artifacts. Radiocarbon-dated stratigraphy indicates a history of receding pluvial lake levels followed by alluvial downcutting and subsequent valley filling with marsh-like conditions at the end of the Pleistocene. A period of alluvial deposition and shallow water tables (9,800 to 11,000 14C yr B.P.) correlates to the Younger Dryas. Subsequent drier conditions and reduced surface runoff mark the early Holocene; sand dunes replace wetlands by 8,000 14C yr B.P. The stratigraphy at Sunshine is similar to sites located 400 km south and supports regional climatic synchroneity in the central and southern Great Basin during the terminal Pleistocene/early Holocene. Given regional climate change and recurrent geomorphic settings comparable to Sunshine, we believe that there is a high potential for buried Paleoindian features in primary association with extinct fauna elsewhere in the region yet to be discovered due to limited stratigraphic exposure and consequent low visibility.