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1 results

  • A monoclonal antibody to Babesia divergens which inhibits merozoite invasion

  • Caroline M. Winger, Elizabeth U. Canning, J. D. Culverhouse
  • Journal:
    Parasitology / Volume 94 / Issue 1 / February 1987
    Published online by Cambridge University Press:
    06 April 2009, pp. 17-27

  • A monoclonal antibody-producing hybridoma cell line has been raised against merozoites of Babesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50–60 K by Western blot analysis on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes.