The IAEA is currently preparing a new Technical Reports Series (TRS) Handbook on the Transfer of Radionuclides to Wildlife. Key deficiencies have been identified and one is the lack of data for birds. In this study we measured the transfer of a range of elements measured by neutron activation analysis (NAA) and gamma analysis in four Barn owls (Tyto alba) and one Tawny owl (Strix aluco) from archived samples available from the Predatory Bird Monitoring Scheme, http://pbms.ceh.ac.uk. Transfer was quantified as the whole organism concentration ratio (CRwo-soil) and values for Co, Fe, Rb, Sc, Zn and 137Cs are presented. The work demonstrates the potential for existing sample archives to be exploited for radioecological purposes. They represent a cost effective means to derive CRwo-media values for organism-radionuclide combinations relevant to radiological assessments without the requirement of field sampling.