Surgeons and bioengineers have continuously been challenged by implant failure. Many of these engineers and surgeons trace implant failure to poor osseointegration (the bonding of an orthopedic implant to juxtaposed bone) and to the inability of implants to match the physical properties of surrounding bone. Researchers have recently shown that nanostructured materials (or materials with fundamental length scales less than 100 nm) enhance cell functions pertinent to effectively regenerating the tissue of numerous organs. Specifically, in a recent study, researchers demonstrated that metal surfaces utilizing low-micron to nanophase topography fostered increased adhesion of osteoblasts, the cells that create the matrix of bone. In this study, Ti, Ti6Al4V, and CoCrMo alloys were investigated, and these alloys were identical to current orthopedic implant alloys except for surface topography. The objective of this in vitro research was to determine whether these same nanophase metal surfaces not only foster osteoblast adhesion but also increase osteoblast metabolic activities leading to bone regeneration. Light microscopy and Energy Dispersion Spectroscopy (EDS) were used to verify the presence of calcium and phosphorous deposition by osteoblasts cultured on the metal substrates. Results indicated that both calcium and phosphorous are being deposited on several of the metal substrates. More importantly, compared to conventional metals, results provided the first evidence that more calcium and phosphorous was deposited by osteoblasts cultured on respective nanophase metals (Ti, Ti6Al4V, and CoCrMo). Nanophase CoCrMo had the most calcium and phosphorous minerals deposited by osteoblasts compared to any other metal substrate. Thus, the results of this study continue to provide evidence for the use of nanophase metals for the design of the next generation of more successful orthopedic implants.