Leishmania infantum immunoelectrophoretic antigen 24 (AG
visceral leishmaniasis associated immunodominant
antigen, has been characterized with a monospecific antiserum by combining
SDS–PAGE, immunoblotting, metabolic
labelling, radio-immunoprecipitation and in vitro poly A+
mRNA translation. AG 24 appeared to correspond to a multi-antigen family
6–9 members ranging from 20 to 31 kDa and proteinic by nature with
no post-translational modifications.
A similar banding pattern was recognized by infection sera. AG 24 was
not found exposed on the cell surface.