The pattern of inheritance of powdery mildew resistance expressed by two bread wheat (cultivar ‘Alcedo’)/Aegilops markgrafii introgression lines was explored using F2 populations bred from crosses made with the powdery mildew-susceptible cultivar ‘Kanzler’. Disease reaction was tested at both seedling and adult plant stages. Two resistance loci, designated QPm.ipk-1A and QPm.ipk-7A, were identified as mapping to the distal ends of chromosome arms 1AS and 7AL, respectively. Whereas QPm.ipk-1A was expressed throughout the plant's life, QPm.ipk-7A was only effective at the seedling stage. The map location of both resistance loci indicated that resistances originated from A. markgrafii. The possible genetic relationship of these disease-resistant genes to known Pm genes is discussed in the context of synteny.