Seasonal distribution and abundance of anchovy eggs were analysed during three scientific cruises carried out from summer 2006 to summer 2007 in the Boka Kotorska Bay, and one cruise in July 2008 in the open waters of the south-eastern Adriatic Sea. Daily egg production method (DEPM) was applied for the first time for estimation of the anchovy spawning stock biomass in the south-eastern Adriatic Sea. The daily egg production was 13–581 eggs day−1 within the bay, and 42–110 eggs day−1 in the open waters. Anchovy egg abundance was highest in spring inside the Bay of Kotor (one of the innermost and eutrophic sub-bays of the Boka Kotorska Bay), which indicates that the conditions for anchovy spawning are very favourable in this part of the Adriatic Sea, even when temperatures are below the optimum. This study has shown that the Boka Kotorska Bay is an area of very intensive spawning of anchovy, and that small-size anchovies live and spawn in the bay, and after spawning migrate towards open waters. The length at which 50% of anchovy were mature (L50) was calculated as 9.28 for females and 9.02 for males. Spawning stock biomass was at a stable level during the period of investigation.