Seedling emergence and stand establishment of crops following the allelopathic crops are often affected. When the crops are grown in rotation, they are influenced by allelopathy of preceding crop. In this study, consisting of three independent experiments, allelopathic effect of tobacco, rich in several allelochemicals, on the subsequent maize and mungbean crops was investigated. In first experiment, maize and mungbean were sown in field previously occupied by tobacco or was fallow. In second experiment, maize and mungbean were planted in pots after the harvest of tobacco. Whereas in third experiment, maize and mungbean seeds were soaked in water or 2 and 4 mM nicotine and were then sown in soil-filled pots. Stand establishment, leaf emergence and growth of maize were significantly improved when grown after tobacco, whereas in mungbean these characters were suppressed when grown after tobacco. Likewise external application of nicotine improved the emergence uniformity, seedling dry weight and chlorophyll contents in maize and suppressed these parameters in mungbean. Tobacco allelopathy also increased the total soil nitrogen, zinc, iron, nicotine and total phenolics. Nicotine-induced change in chlorophyll contents and α-amylase activity seems the possible reason of differential response of maize and mungbean to tobacco allelopathy. When grown in rotation with tobacco, stand establishment and growth of maize were improved, whereas mungbean stand and growth were suppressed. In crux, allelopathic nature of the crops must be considered while making the crop rotations.