Cottonseed Meal (CSM) is a byproduct of the process used to extract oil from cotton seeds and contains 28- 35% crude protein in the Iran, depending on the amount of hull separation prior to the oil extraction process. Although CSM is high in protein, due to its low lysine levels and presence of toxic substance, gossypol, its utilization in poultry feed as a protein supplement is limited. Problems related to lysine and nutrient density are easily rectified by addition of synthetic lysine to poultry diets. However, solutions to the problems related to gossypol in CSM have been elusive. Gossypol, a naturally occurring anti-nutritional factor that is reduced broiler performance and increased their mortality (Smith, 1970). However, it has been shown that several factors, such as age, strain of chicks, dietary iron, and dietary lysine may affect birds’ tolerance to gossypol (Clawson and Smith, 1966; Martin, 1990). The objective of this research was to evaluate the response of broiler performance when high levels of CSM were offered with Lysine (Lys), Ferrous sulphate (FS), and both in diet.