The current study aimed to evaluate the effects of cactus pear as a moistening additive on fermentative and microbiological characteristics, aerobic stability (AS), chemical composition and in situ rumen degradability of corn grain silage at different opening times. A completely randomized experimental design was adopted in a 4 × 3 factorial scheme with four levels of dry matter (DM) (50; 60; 70 and 80% of DM) and three opening times (30; 60 and 120 days after ensiling), with four replications. There was an effect of interaction (P < 0.05) between the DM levels and opening times on silage yeast population, effluent losses, gas losses, dry matter recovery (DMR), AS of the silage and on lactic acid bacteria, mould and yeast populations after AS trial. The 60% DM level presented DMR values above 930 g/kg of DM. However, the lowest AS time (96.52 h) was observed in silages with 60% DM at 60 days after ensiling, although all silages have shown high AS. The DM in situ degradability of the ensiled mass increased after the ensiling process at all DM levels and opening times, with the 60% DM content showing the best result. When using cactus pear as a corn grain moistening additive, the 60% DM level is recommended when the opening time is up 120 days.

Embryo development in eggs of the spittlebug Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae) passes through four phases (known as S1 to S4) being stopped at S2 during diapause. Studies about the molecular basis of diapause in spittlebugs are nonexistent. Here, we analyzed proteins from non-diapausing (ND), diapausing (D) and post-diapausing (PD) eggs of the spittlebug M. spectabilis. In total, we identified 87 proteins where 12 were in common among the developmental and diapause phases and 19 remained as uncharacterized. Non-diapausing eggs (S2ND and S4ND) showed more proteins involved in information storage and processing than the diapausing ones (S2D). Eggs in post-diapausing (S4PD) had a higher number of proteins associated with metabolism than S2D. The network of protein interactions and metabolic processes allowed the identification of different sets of molecular interactions for each developmental and diapause phases. Two heat shock proteins (Hsp65 and Hsp70) along with two proteins associated with intracellular signaling (MAP4K and a serine/threonine-protein phosphatase) were found only in diapausing and/or post-diapausing eggs and are interesting targets to be explored in future experiments. These results shine a light on one key biological process for spittlebug survival and represent the first search for proteins linked to diapause in this important group of insects.

Tall fleabane [Conyza sumatrensis (Retz.) E. Walker] is commonly invasive in agricultural fields, reducing yield in various infested crops. The current study investigates the genetic diversity within and between a significant number of invasive C. sumatrensis biotypes in soybean fields in southern, southeastern, and midwestern Brazil, using microsatellites as molecular markers. High and low observed and expected heterozygosity estimated in microsatellite loci supported our hypothesis that different levels of genetic diversity may be detected within biotypes from different invaded fields. Analysis of a significant number of biotypes in several fields showed high and low genetic diversity not associated with geographic distribution, bottleneck effect, or susceptibility to glyphosate. A deficit of heterozygous plants, high genetic divergence, and moderate allelic transference were also observed. Allelic fixation was different in the different biotypes. The bottleneck effect was seen in biotypes with reduced genetic diversity and in biotypes with the highest genetic diversity. Data on genetic diversity, bottleneck effect, and glyphosate resistance showed contrasts in biotypes from nearby invaded fields. Our study showed different genetic diversity levels in biotypes from invaded areas under the same climatic conditions.

The diagnosis of visceral leishmaniasis (VL) has improved with the search of novel antigens; however, their performance is limited when samples from VL/human immunodeficiency virus (HIV)-coinfected patients are tested. In this context, studies conducted to identify more suitable antigens to detect both VL and VL/HIC coinfection cases should be performed. In the current study, phage display was performed using serum samples from healthy subjects and VL, HIV-infected and VL/HIV-coinfected patients; aiming to identify novel phage-exposed epitopes to be evaluated with this diagnostic purpose. Nine non-repetitive and valid sequences were identified, synthetized and tested as peptides in enzyme-linked immunosorbent assay experiments. Results showed that three (Pep2, Pep3 and Pep4) peptides showed excellent performance to diagnose VL and VL/HIV coinfection, with 100% sensitivity and specificity values. The other peptides showed sensitivity varying from 50.9 to 80.0%, as well as specificity ranging from 60.0 to 95.6%. Pep2, Pep3 and Pep4 also showed a potential prognostic effect, since specific serological reactivity was significantly decreased after patient treatment. Bioinformatics assays indicated that Leishmania trypanothione reductase protein was predicted to contain these three conformational epitopes. In conclusion, data suggest that Pep2, Pep3 and Pep4 could be tested for the diagnosis of VL and VL/HIV coinfection.

The nanoencapsulation of biocomposites with anthelmintic action has been proposed as an alternative for improving their efficiency. Thus, the current study aimed to evaluate the efficacy of carvacryl acetate nanoencapsulated with biopolymers (nCVA) in the control of sheep gastrointestinal nematodes. CVA was nanoencapsulated with chitosan/chichá gum and characterized in terms of its efficacy of encapsulation (EE), yield and zeta potential. The acute toxicity of nCVA was evaluated in mice. For the fecal egg count reduction test, 40 animals were divided into four groups (n = 10) and orally administered the following treatments: G1, 250 mg kg−1 CVA; G2, 250 mg kg−1 nCVA; G3, chitosan/chichá gum (negative control) and G4, 2.5 mg kg−1 monepantel (positive control). Feces were collected on days 0 and 16 posttreatment to determine the eggs per gram of feces (epg). The EE and yield of nCVA were 72.8 and 57.5%, respectively. The nanoparticles showed a size of 764.5 ± 302.5 nm, and the zeta potential at pH 3.2 was +22.0 mV. nCVA presented a 50% lethal dose (LD50) of 2609 mg kg−1. By 16 days posttreatment, CVA, nCVA and monepantel reduced the epg by 52.9.7, 71.5 and 98.7%, respectively, and the epg of sheep treated with nCVA differed from that of the negative control (P > 0.05) but did not differ from that of sheep treated with CVA. In conclusion, the nanoencapsulation of CVA reduced its toxicity, and nCVA showed anthelmintic activity.

In this work, two-phase flows of Newtonian and/or viscoelastic fluids in a ‘cross-slot’ geometry are investigated both experimentally and numerically in the creeping-flow limit. A series of microfluidic experiments – using Newtonian fluids – have been carried out in different cross-section aspect ratios to support our numerical simulations. The numerical simulations rely on a volume of fluid method and make use of a log-conformation formulation in conjunction with the simplified viscoelastic Phan-Thien and Tanner model. Downstream from the central cross, once the flow has become fully developed, we also estimate analytically the thickness of each fluid layer for both two- and three-dimensional cases. In addition to providing a benchmark test for our numerical solver, these analytical results also provide insight into the role of the viscosity ratio. Injecting two fluids with different elastic properties from each inlet arm is shown to be an effective approach to stabilize the purely elastic instability observed in the cross-slot geometry based on the properties of the fluid with the larger relaxation time. Our results show that interfacial tension can also play an important role in the shape of the interface of the two fluids near the free-stagnation point (i.e. in the central cross). By reducing the interfacial tension force, the interface of the two fluids becomes curved and this can consequently change the curvature of streamlines in this region which, in turn, can modify the purely elastic flow transitions. Thus, increasing interfacial tension is shown to have a stabilizing effect on the associated steady symmetry-breaking purely elastic instability. However, at high values of the viscosity ratio, a new time-dependent purely elastic instability arises most likely due to the change in streamline curvature observed under these conditions. Even when both fluids are Newtonian, outside of the two-dimensional limit, a weak instability arises such that the fluid interface in the depth (neutral) direction no longer remains flat.

Pork is one of the most consumed meats worldwide, yet, pork quality remains an issue for the industry, mainly because of flavour, colour and water holding capacity instabilities. Castration techniques combined with dietary supplementation with ractopamine hydrochloride (RAC) seem to be a tangible solution to deal with these issues. There is a lack of knowledge of how these techniques will impact the proteomic profile and, consequently, the meat quality. The main goal of this work was to study the proteomic profile of non-aged pork meat under different sexual conditions and RAC dietary supplementation, and how the combination of these two factors impacts meat quality attributes. Forty-eight animals were distributed in six treatments, three sexual conditions (females; surgically castrated males; immune castrated males) and two diets (with RAC inclusion or without). For proteomic analysis, a sample of the Longissimus dorsi muscle was collected 24 h after slaughter and analysed using one-dimensional SDS/PAGE. The ultimate pH and colour (L*, a*, b*) were measured in the carcasses after 24 h, then meat samples were collected to measure drip, cooking and thawing losses, as well as the shear force. The interaction between gender and diet affected the ultimate pH and the L* parameter. Meat tenderness was only influenced by diet. Twenty-seven protein bands were revealed by SDS/PAGE, six of them with the protein abundance affected by diet. In conclusion, the inclusion of dietary RAC caused differences in the pork meat proteome, and more studies are necessary to fully explore the proteins involved in these changes.

Cactus pear is an important species for animal feeding in the regions of dry climate. There is no information on the fertilization with coated urea in the cultivation of cactus pear under rainfed conditions in the savannah region. The aim of the current study was to evaluate the forage potential of Nopalea cochenillifera variety Doce in yellow latosol under rainfed conditions in the Brazilian savannah, comparing the fertilization with urea and coated urea in different levels. A randomized block design was adopted, in a 2 × 4 × 2 factorial scheme, with the factors corresponding to two sources of nitrogen (urea and urea coated with polymers, N+), four levels of nitrogen (0, 60, 120 and 240 kg/ha/year) and two harvests (year I and year II). The plants were evaluated after 1 year of growth, in each year of evaluation, regarding the characteristics of growth, production, chemical and mineral composition and nutritional value. The level of 240 kg/ha provided higher emission of cladodes per plant (17.33 and 18.17), respectively, for N+ and urea. The highest nitrogen use efficiency was found in the level of 60 kg N/ha (142 kg/ha/year). NFC values were 3.5 g/kg dry matter (DM) higher when the cactus pear was fertilized with urea in year I and 5.4 g/kg DM in year II. The use of conventional urea promoted better results of agronomic and nutritional characteristics of the cactus pear, under rainfed regime, when compared to the use of urea coated with polymers.

This study aimed to determine the requirements of standardized ileal digestible (SID) methionine (Met) + cysteine (Cys) of 15–30 kg barrows, maintaining or increasing the 1:1 ratio between SID Met and SID Cys. Seventy crossbred barrows averaging 15.2 ± 0.54 kg of live weight were allotted in a randomized block design 2 × 3 + 1 factorial scheme, with five replicates and two animals per pen. Treatments consisted of maintaining or increasing the 1:1 ratio between SID Met and SID Cys, three levels of SID Met + Cys (5.8, 6.4 and 7.0 g/kg) and a basal diet containing the lowest SID Met + Cys level (5.2 g/kg), formulated to provide a 1:1 ratio of SID Met (2.6 g/kg) and SID Cys (2.6 g/kg). Performance, blood parameters, longissimus dorsi muscle depth, backfat thickness and S–S linkages in the bristles were evaluated. The best average daily gain was estimated at 6.61 g/kg of SID Met + Cys, without maintaining the 1:1 ratio between sulphur amino acids (SAA). The daily intake of SID Met + Cys increased due to dietary SID Met + Cys levels, maintaining or increasing the same ratio between SAA. Plasma glucose increased and total cholesterol decreased according to SID Met + Cys levels, without maintaining the same ratio between the SAA. The requirement of SID Met + Cys for 15–30 kg barrows was 6.61 g/kg (7.88 g/day) for an optimum average daily gain, with no need to maintain the same ratio between the SAA.

Identifying dietary patterns in different environments attended by children is relevant to guide public politics. The aim of this study was to analyse the factors associated with dietary patterns of children under 2 years of age in childcare centres and at homes. This transversal study was enrolled in municipal childcare centres of Guaratuba, Paraná, Brazil. Food consumption data from 256 children were obtained by the food record method. From the consumption data, four dietary patterns were identified by factor analysis using the principal component method in each environment. ‘Traditional’ patterns were observed in both environments; the ‘less healthy’ pattern was found only at homes. Other patterns identified in childcare centres include ‘snacks’, ‘nutritive’ and ‘pasta and meats’; at homes, it was possible to identify patterns such as ‘milk and cereals’ and ‘mixed’. Children over 12 months presented higher scores for all the patterns in both environments. Obese children had lower scores for all the patterns in childcare centres. There was an association between maternal age below 21 years and higher adherence to a ‘less healthy’ pattern, maternal level of education less than 8 years and lower adherence to the ‘mixed’ pattern and lower familiar income per capita and higher adherence to the ‘snacks’ and ‘traditional’ patterns in childcare centres. In conclusion, adherence to dietary patterns was associated with socio-economic, demographic variables and nutritional status and further studies are needed, especially those with a longitudinal design, enabling the monitoring of dietary patterns.

Diaphragmatic eventration is an anomaly of the diaphragm. In Scimitar syndrome, a curved-shaped anomalous pulmonary venous drainage is seen. Association between these conditions is rare. We present a newborn with diaphragmatic eventration, whose diagnosis of Scimitar syndrome was made after surgical repair. Scimitar syndrome is a congenital disorder often associated with other heart and lungs anomalies. Diagnosis can be fortuitous but with important prognostic features.

We investigated the effects of pathogens associated with subclinical intramammary infections on yield, composition and quality indicators of goat milk. By means of a longitudinal study, individual half udder milk samples (n = 132) were collected at different lactation periods and assessed for milk yield and physicochemical composition, somatic cell count (SCC), total bacteria count (TBC) and microbiological culture. Staphylococci species accounted for the great majority of the isolates (96.1%). Intramammary infections significantly reduced fat and total solids in goat milk and increased both SCC and TBC. However, these indicators were significantly higher in udder halves affected by S. aureus compared with other staphylococci species.

The retention of human milk (HM) fat in nasogastric probes of infusion pumps can be observed during the feed of infants unable to suck at the mother’s breast. The lack of homogenisation of HM could contribute to the fat holding. Therefore, the present study evaluated (i) the influence of homogenisation on milk fat retaining in infant feeding probes and (ii) the in vivo effect of the homogenisation on lipid absorption by Wistar rats. The animals were fed with HM treated following two processing conditions, that is, pasteurised and homogenised–pasteurised. The animals were randomly subdivided into four experimental groups: water-fed (control), pasteurised milk, homogenised–pasteurised milk and pasteurised–skimmed milk. The results of food consumption, mass body gain, corporate metrics and plasma blood levels of total cholesterol did not show any difference (P < 0·05) among the three types of HM used in the experiments. The liver, intestine and intra-abdominal adipose tissue of the four groups of animals presented normal and healthy histology. The composition of fatty acids in the brain tissue of animals fed with homogenised HM increased when compared with the groups fed with non-homogenised HM. These values were 11·08 % higher for arachidonic acids, 6·59 % for DAH and 47·92 % for nervous acids. The ingestion of homogenised HM promoted higher absorption of milk nutrients. Therefore, the addition of the homogenisation stage in HM processing could be an alternative to reduce fat retention in probes and to improve the lipids’ absorption in the body.

Human ascariasis is the most common and prevalent neglected tropical disease and is estimated that ~819 million people are infected around the globe, accounting for 0.861 million years of disability-adjusted life years in 2017. Even with the existence of highly effective drugs, the constant presence of infective parasite eggs in the environment contribute to a high reinfection rate after treatment. Due to its high prevalence and broad geographic distribution Ascaris infection is associated with a variety of co-morbidities and co-infections. Here, we provide data from both experimental models and humans studies that illustrate how complex is the interaction of Ascaris with the host immune system, especially, in the context of reinfections, co-infections and associated co-morbidities.

Seeds of Papilionoideae (Leguminosae) species lose water after dispersal, increasing the proportion of water-impermeable seeds (physical dormancy, PY). However, changes in testa structure during this process remain unknown. The present study investigated how variation in relative humidity (RH) during the post-dispersion period affects the proportion of seeds of Erythrina speciosa with PY. Seeds from two populations were stored in drier (40% RH) and wetter (80% RH) environments and periodically subjected to physiological analysis (seed water content, imbibition and germination) and structural analysis of the testa (light and scanning electron microscopy). Drier storage resulted in seed dehydration, increasing the proportion of water-impermeable seeds and closure of cracks in the mucilaginous stratum. In contrast, wetter storage led to an increase in seed water content, a decrease in the proportion of seeds with PY, and the formation of cracks. As a result of variation in environmental humidity, we conclude that changes occur in the mucilaginous stratum of seeds, altering water loss and, consequently, the proportion of seeds with PY. Environments with low humidity cause a decrease in seed water content, the closing of cracks in the mucilaginous stratum, and, consequently, an increase in the proportion of water-impermeable seeds. On the other hand, a high RH environment increases the seed water content and the formation of superficial cracks, through which water enters during imbibition, causing a decrease in the proportion of seeds with PY.

Deaf people communicate naturally using visual-spatial languages, called sign languages (SL). Although SLs are recognized as a language in many countries, the problems faced by Deaf people for accessing information remain. As a result, they have difficulties exercising their citizenship and access information in SLs, which usually leads to linguistic and knowledge acquisition delays. Some scientific works have been developed to address these problems related to the machine translation of spoken languages to sign languages. However, the existing machine translation platforms have some limitations, especially in syntactic and lexical nature. Thus, this work aims to develop a mechanism for machine translation to Libras, the Brazilian Sign Language, with syntactic-semantic adequacy. It consists of an automatic translation component for Libras based on syntactic-semantic translation rules and a formal syntactic-semantic rule description language. As proof of concept of the proposed approach, we created a specific grammar for Libras translation exploring these aspects and integrating these elements into VLibras Suite, a service for machine translation of digital content in Brazilian Portuguese (BP) to Libras. We performed several tests using this modified version of VLibras to measure the level of comprehension of the output generated by the new translator mechanism. In the computational experiments, as well as in the actual tests with Deaf and hearing users, the proposed approach was able to improve the results of the current VLibras version.

Calculi with disjoint intersection types support a symmetric merge operator with subtyping. The merge operator generalizes record concatenation to any type, enabling expressive forms of object composition, and simple solutions to hard modularity problems. Unfortunately, recent calculi with disjoint intersection types and the merge operator lack a (direct) operational semantics with expected properties such as determinism and subject reduction, and only account for terminating programs. This paper proposes a type-directed operational semantics (TDOS) for calculi with intersection types and a merge operator. We study two variants of calculi in the literature. The first calculus, called λi, is a variant of a calculus presented by Oliveira et al. (2016) and closely related to another calculus by Dunfield (2014). Although Dunfield proposes a direct small-step semantics for her calculus, her semantics lacks both determinism and subject reduction. Using our TDOS, we obtain a direct semantics for λi that has both properties. The second calculus, called λi+, employs the well-known subtyping relation of Barendregt, Coppo and Dezani-Ciancaglini (BCD). Therefore, λi+ extends the more basic subtyping relation of λi, and also adds support for record types and nested composition (which enables recursive composition of merged components). To fully obtain determinism, both λi and λi+ employ a disjointness restriction proposed in the original λi calculus. As an added benefit the TDOS approach deals with recursion in a straightforward way, unlike previous calculi with disjoint intersection types where recursion is problematic. We relate the static and dynamic semantics of λi to the original version of the calculus and the calculus by Dunfield. Furthermore, for λi+, we show a novel formulation of BCD subtyping, which is algorithmic, has a very simple proof of transitivity and allows for the modular addition of distributivity rules (i.e. without affecting other rules of subtyping). All results have been fully formalized in the Coq theorem prover.

We report results and modelling of an experiment performed at the Target Area West Vulcan laser facility, aimed at investigating laser–plasma interaction in conditions that are of interest for the shock ignition scheme in inertial confinement fusion (ICF), that is, laser intensity higher than ${10}^{16}$ $\mathrm{W}/{\mathrm{cm}}^2$ impinging on a hot ($T>1$ keV), inhomogeneous and long scalelength pre-formed plasma. Measurements show a significant stimulated Raman scattering (SRS) backscattering ($\sim 4\%{-}20\%$ of laser energy) driven at low plasma densities and no signatures of two-plasmon decay (TPD)/SRS driven at the quarter critical density region. Results are satisfactorily reproduced by an analytical model accounting for the convective SRS growth in independent laser speckles, in conditions where the reflectivity is dominated by the contribution from the most intense speckles, where SRS becomes saturated. Analytical and kinetic simulations well reproduce the onset of SRS at low plasma densities in a regime strongly affected by non-linear Landau damping and by filamentation of the most intense laser speckles. The absence of TPD/SRS at higher densities is explained by pump depletion and plasma smoothing driven by filamentation. The prevalence of laser coupling in the low-density profile justifies the low temperature measured for hot electrons ($7\!{-}\!12$ keV), which is well reproduced by numerical simulations.