Two species of the genus Aporcella, one new and one previously known, collected from cultivated fields in Iran are studied. Description, morphometrics, illustrations (both line and microphotographs) and D2–D3 sequences are provided for Aporcella talebii sp. n., which is characterized by its 1.66–2.02-mm-long body, lip region offset by constriction and 15–17 μm broad, odontostyle 14–17.5 μm long, neck 412–484 μm long, pharyngeal expansion occupying 46–50% of total neck length, uterus simple and 1.6–2.0 times the corresponding body diameter long, V = 52–59, tail conical (40–50 μm, c = 37–47, c′ = 1.1–1.4) with a weak but perceptible dorsal concavity at the end and male absent. Morphometrics, microphotographs and D2–D3 sequences of Aporcella simplex are also presented, this being its first Asian record. Molecular analyses confirm the monophyly of the genus, its close relationship with other taxa lacking pars refringens vaginae and the polyphyly of Aporcelaimidae.