Background: Inflammatory bowel disease is an autoimmune disease that affects the gut. T. spiralis larvae (E/S Ags) loaded on calcium-benzene-1,3,5-tricarboxylate metal-organic frameworks (Ca-BTC MOFs) were tested to determine whether they might prevent or cure acetic acid-induced murine colitis. Methods: T. spiralis larvae E/S Ags/Ca-BTC MOFs were used in prophylactic and therapeutic groups to either precede or follow the development of murine colitis. On the seventh day after colitis, mice were slaughtered. The effect of our target antigens on the progress of the colitis was evaluated using a variety of measures, including survival rate, disease activity index, colon weight/bodyweight, colon weight/length) ratios, and ratings for macroscopic and microscopic colon damage. The levels of inflammatory cytokines (interferon-γ and interleukin-4), oxidative stress marker malondialdehyde, and glutathione peroxidase in serum samples were evaluated. Foxp3 T-reg expression was carried out in colonic and splenic tissues. Results: T. spiralis larvae E/S Ags/Ca-BTC MOFs were the most effective in alleviating severe inflammation in murine colitis. The survival rate, disease activity index score, colon weight/length and colon weight/bodyweight ratios, and gross and microscopic colon damage scores have all considerably improved. A large decrease in proinflammatory cytokine (interferon-γ) and oxidative stress marker (malondialdehyde) expression and a significant increase in interleukin-4 and glutathione peroxidase expression were obtained. The expression of Foxp3+ Treg cells was elevated in colonic and splenic tissues. Conclusion: T. spiralis larvae E/S Ags/Ca-BTC MOFs had the highest anti-inflammatory, antioxidant, and cytoprotective capabilities against murine colitis and might be used to develop new preventative and treatment strategies.

Scholars of modern South Asia have remained divided on the role of religion in the creation of Pakistan. Many have argued that Pakistan's “founder,” Muhammad Ali Jinnah, was a secularist, his argument for Pakistan resting on an abstract notion of Islam within an Enlightenment framework of conceiving minority, nation, and state. Why, then, did madrasa-trained Muslim scholars, the ulama, support his demand for Pakistan? This article explores the political thought of the most influential Muslim scholar immediately before partition, Shabbir Ahmad Usmani (d. 1949). I argue that Usmani viewed Pakistan as a particular kind of Islamic democracy. While he drew on medieval Muslim juridical and political discourses, Usmani's readings reveal his debt to Western political categories. By paying attention to the tensions and opportunities offered by this encounter of modern political conditions with Islamic intellectual thought, this article outlines an Islamic vision of the political that resonates beyond the politics of colonial India.

Boundary-layer disturbances are analysed on a $5^{\circ }$ half-angle blunted cone in Mach 5, high-enthalpy flow ($h_0 = 9\ {\rm MJ}\ {\rm kg}^{-1}$) with a low wall-to-edge temperature ratio, $T_w/T_e = 0.18$. Schlieren and focused laser differential interferometry (FLDI) are utilized to assess the structures and frequency content associated with disturbances. Wave packets are identified from bursts of modal content on time-resolved spectrograms. Bandpass filtering, proper orthogonal decomposition (POD) and space–time POD are then applied to the schlieren data. Bandpass filtering suggests the presence of wave packet dispersion and elongation indicative of slow-acoustic-wave synchronization. Modal reconstruction techniques indicate the radiation of content outside the boundary layer and distinct orientation changes within disturbances, potentially the first experimental evidence of the supersonic-mode instability in such a flow field. Cross-bicoherence computations are carried out for discrete time segments of data from both schlieren and FLDI data. They demonstrate that the most dominant nonlinear interactions are the fundamental–first-harmonic and the fundamental–low-frequency interactions.

While effective institutional practices are critical for the evolution of peace certain factors deter their effectiveness. In-group and out-group dynamics may make peace difficult between culturally distinct groups. Critical ecological conditions often lead to intractable conflict over resources. And within group conflicts of interest most prominently between generations may inhibit effective peace making.

In the Himalaya, ice thickness data are limited, and field measurements are even scarcer. In this study, we employed the GlabTop model to estimate ice reserves in the Jhelum (1.9 ± 0.6 km3) and Drass (2.9 ± 0.9 km3) sub-basins of the Upper Indus Basin. Glacier ice thickness in the Jhelum ranged up to 187 ± 56 m with a mean of ~24 ± 7 m, while the Drass showed ice thickness up to 202 ± 60 m, with a mean of ~17 ± 5 m. Model results were validated using Ground Penetrating Radar measurements across four profiles in the ablation zone of the Kolahoi glacier in the Jhelum and nine profiles across the Machoi glacier in the Drass sub-basin. Despite underestimating ice-thickness by ~10%, the GlabTop model effectively captured glacier ice-thickness and spatial patterns in most of the profile locations where GPR measurements were taken. The validation showed high correlation coefficient of 0.98 and 0.87, low relative bias of ~ −13% and ~ −3% and a high Nash–Sutcliffe coefficient of 0.94 and 0.93 for the Kolahoi and Machoi glaciers, respectively, demonstrating the model's effectiveness. These ice-thickness estimates improve our understanding of glacio-hydrological, and glacial hazard processes over the Upper Indus Basin.

Flow-induced vibration (FIV) of a flexible cylinder with a triangular cross-section, allowed to oscillate in the cross-flow, inline and torsional direction, is studied experimentally through water tunnel tests. The dynamic response of the cylinder was studied for three different angles of attack ($0^\circ, 30^\circ, 60^\circ$), at reduced velocities of 0.9–16.27, corresponding to Reynolds numbers of 364–3600. At the angle of attack of $0^\circ$, vortex-induced vibration at low reduced velocity was observed, which transitioned to galloping at higher reduced velocities. At the angles of attack of $30^\circ$ and $60^\circ$, galloping-type response was observed over the range of the reduced velocities tested. Our results show that the cylinder's torsional oscillation breaks the system's symmetry and affects the structural response at higher reduced velocities regardless of the angle of attack. The FIV response of the flexible triangular cylinder is distinct from that of a rigid flexibly mounted triangular cylinder due to torsional oscillation, spanwise flexibility and the two fixed boundary conditions limiting the amplitude of oscillation. Flow field analysis in the wake of the cylinder was done qualitatively and quantitatively using hydrogen bubble flow visualisation and time-resolved volumetric particle tracking velocimetry techniques, respectively. Our results show the existence of highly three-dimensional vortex structures in the wake of the cylinder. We studied the coupling between the vortex shedding modes in the wake of the cylinder and the structural vibration modes through the spatiotemporal mode analysis using the proper orthogonal decomposition technique to distinguish between different types of the FIV response observed.

Background. DNA barcoding is a useful technique for the identification, conservation, and diversity estimation at the species level in plants. The current research work was carried out to characterize selected Fragaria species from northern Pakistan using DNA barcode markers. Methodology. Initially, the efficacy of eight DNA barcode markers was analyzed based on the amplification and sequencing of the genome of selected Fragaria species. The resultant sequences were analyzed using BLAST, MEGA 7.0, and Bio Edit software. The phylogenetic tree was constructed by using Fragaria current species sequences and reference sequences through the neighbor-joining method or maximum likelihood method. Results. Among eight DNA barcode markers, only two (ITS2 and rbclC) were amplified, and sequences were obtained. ITS2 sequence was BLAST in NCBI for related reference species which ranged from 89.79% to 90.05% along with Fragaria vesca (AF163517.1) which have 99.05% identity. Similarly, the rbclC sequence of Fragaria species was ranged from 96% to 99.58% along with Fragaria × ananassa (KY358226.1) which had 99.58% identity. Conclusion. It is recommended that DNA barcode markers are a useful tool to identify the genetic diversity of a species. Moreover, this study could be helpful for the identification of the Fragaria species cultivated in other regions of the world.

Transition nuclear proteins (TNPs), the principal proteins identified in the condensing spermatids chromatin, have been found to play a key role in histone displacement and chromatin condensation during mammalian spermatogenesis. One such gene belonging to the TNP family called TNP1 gene is abundantly expressed in the regulation of spermatogenesis, and its sequence is remarkably well conserved among mammals. Genomic analysis, by sequencing and computational approach, was used to identify the novel polymorphisms and to evaluate the molecular regulation of TNP1 gene expression in Sahiwal cattle breeding bulls. DNA samples were sequenced to identify novel single nucleotide polymorphisms (SNPs) in the TNP1 gene. Modern computational tools were used to predict putative transcription factor binding in the TNP1 promoter and CpG islands in the TNP1 promoter region. In the TNP1 gene, four SNPs, three TATA boxes, and one CAAT box were identified. One CAAT box was discovered at 89 bp upstream of start site ATG. The computational analyses indicated that the polymorphisms inside the promoter sequence results in an added HNF-1 transcription factor binding site. In contrast, the other variations may remove the naturally occurring SRF transcription factor binding site. The CpG islands in the TNP1 promoter region were predicted to be absent by the MethPrimer program before and after SNP site mutations. These findings pave the way for more research into the TNP1 gene’s promoter activity and the links between these SNPs and reproductive attributes in the Sahiwal breeding bulls.

The hangul Cervus hanglu hanglu, a Critically Endangered mountain ungulate of Jammu and Kashmir, India, faces the imminent threat of population loss and extinction. Effective management of its largest viable population in Dachigam National Park in the Kashmir Himalaya requires reliable demographic information. Using 14 microsatellite markers we identified 293 individuals (208 females and 85 males) through faecal analysis, and generated data on the genetic status and population size of the hangul in its winter habitat. The mean expected and observed heterozygosities of 0.62 and 0.59 are comparable to those of several red deer Cervus elaphus populations elsewhere. The effective population sizes were 46.3 and 93.7 when the frequencies of rare alleles were considered to be 0.050 and 0.010, respectively. The average mean kinship of the population was 0.34, and there was no evidence of a recent bottleneck event. In genetic mark–recapture analysis the best model included an effect of sex on both detection and recapture probabilities. Detection of males was highest in November, coinciding with the hangul breeding season, whereas detection of females was highest in December. Our estimate of the hangul population using genetic mark–recapture with bootstrapping was 394 individuals. To our knowledge, this is the first study to use genetic data to estimate the population of the hangul. It will guide future studies of this subspecies and also serve as an impetus for identifying founder animals for captive breeding, and for connecting the population in Dachigam National Park with the other small, isolated populations to ensure the long-term survival of this subspecies.

Nemavermes mackeei Schram, 1973, found in the Mazon Creek fossil site and the Bear Gulch Limestone, was described initially as a free-living marine nematode. Here we investigate 13 specimens of N. mackeei from the Mazon Creek to reassess its morphology and identity, and also two specimens originally identified as Gilpichthys greenei Bardack and Richardson, 1977. Based on the extensive morphological variation among these specimens, N. mackeei encompasses multiple species that are only distantly related. The holotype of N. mackeei is a proboscis of Tullimonstrum gregarium Richardson, 1966, making N. mackeei a junior synonym of T. gregarium. However, the other specimens that we investigated could not be attributed to T. gregarium. We name a new species from these specimens: Squirmarius testai new genus new species, a cyclostome. One specimen is likely a juvenile G. greenei. Other specimens were not identified during this study but represent a variety of vermiform bilaterians.

UUID: http://zoobank.org/d8c63f6a-0ef4-4a34-8dbd-c1d9099589cc

Trichinellosis is a global food-borne disease caused by viviparous parasitic nematodes of the genus Trichinella. Due to the lack of effective, safe therapy and the documented adverse effects of traditional therapy, this study aimed to evaluate the therapeutic effect of acetazolamide-loaded silver nanoparticles (AgNPs) on murine trichinellosis. Fifty male Swiss albino mice were divided into five groups of ten mice each: Group I, normal control group; Group II, infected with T. spiralis and not treated; Group III, infected and given AgNPs; Group IV, infected and treated with acetazolamide; and Group V, infected and treated with acetazolamide-loaded AgNPs. Mice were infected orally with 250 larvae. The efficacy was assessed by counting T. spiralis adults and larvae, measuring serum total antioxidant capacity, and observing the histopathological and ultrastructural alterations. Acetazolamide-loaded AgNPs treatment exhibited the highest percentage of reduction (84.72% and 80.74%) for the intestinal adults and the muscular larvae of T. spiralis-infected animals, respectively. Furthermore, during the intestinal and muscular phases, the serum of the same group had the best free-radical scavenging capacity (antioxidant capacity), which reduced tissue damage induced by oxidative stress. Histopathologically, the normal intestinal and muscular architecture was restored in the group treated with acetazolamide-loaded AgNPs, in addition to the reduced inflammatory infiltrate that alleviated inflammation compared to infected animals. Our results confirmed the marked destruction of the ultrastructural features of T. spiralis adults and larvae. Acetazolamide-loaded AgNPs are a promising therapy against T. spiralis infection.

This paper presents a low-profile six-beam antenna implemented by a compact two-layer 6 × 6 beamforming network (BFN) and a 6 × 2 slot antenna in substrate integrated waveguide (SIW) technology. The main components of the proposed 6 × 6 BFN are 3 × 3 multi-aperture couplers, interlayer hybrid couplers, and several phase shifters which are embedded on two microwave substrates. The proposed antenna has been designed, simulated, and fabricated for the frequency range of 28–32 GHz. The size of this antenna is 82 × 31.8 × 0.787 mm3, which can be a suitable choice for 5G applications due to its compact dimensions compared to similar works. Prototype testing shows that the proposed structure presents a stable beamforming performance both in simulation and measurement with good agreement. The antenna generates six radiation beams in directions ±9°, ±30°, and ±54° with good return losses and isolations.

Research suggests that adiponectin, leptin, and genetic polymorphisms such as catechol-O-methyltransferase (COMT) genotype may play an integral role in blood pressure status and thereby cardiovascular health. This is an area especially important for women who are post-menopause; however, the current literature investigating these associations is limited. This study was a cross-sectional secondary analysis of baseline data (N 237) from the Minnesota Green Tea Trial (MGTT). The current study explored the relationships between plasma adiponectin, leptin, and COMT genotype on blood pressure measures. Plasma adiponectin and leptin were obtained after an overnight fast of at least 10 h and were measured by the radioimmunoassay method. The relationships were analysed using multiple linear regression after adjusting for potential confounders. Effect modifications by age, body mass index (BMI) category, blood pressure category, antihypertensive medication use, and COMT genotype were also investigated. The majority of participants were non-Hispanic (97⋅9 %) and Caucasian (94⋅9 %). Mean (sd) age and BMI were 60⋅7 (5⋅0) years and 28⋅2 (2⋅9) kg/m2, respectively. After adjustment for confounding variables, neither plasma adiponectin, plasma leptin nor COMT genotype was associated with systolic or diastolic blood pressure measures. The results of stratified analyses also did not reveal any significant interactions or associations. Based on the findings of this study, which utilised more rigorous statistical methods than previous research, neither adiponectin, leptin nor COMT genotype play a role in blood pressure measures in women who are post-menopause.

Population size and geographical range are the key quantitative criteria used by the IUCN to assess the conservation status of a species. However, such information is often incomplete and inconsistent, even for seemingly abundant species. To assess the population and conservation status of Indian primates, we conducted a systematic review of recent research using the searching, appraisal, synthesis and analysis (SALSA) approach. We reviewed a total of 41 studies on Indian primates conducted during the last 2 decades (2000–2021) for information on various parameters that influence their conservation. We found that 20 out of a total of 26 primate species were evaluated for their population status, and the majority of these studies (71%) showed an overall declining population trend. Remarkably, all but one of the studies conducted exclusively within protected areas revealed declining population trends, whereas trends were more variable for primate populations in non-protected areas. Our data indicate that only 27% (n = 7) of Indian primate species have been surveyed or re-surveyed to assess their population status within the last 5 years. Although threats vary in time and space from species to species, 78% of the studies recorded natural system modifications including habitat loss and fragmentation among the main threats to the survival of Indian primates. Most studies on the population status of Indian primates have either been spatially limited or used outdated methods. We recommend that future studies adopt robust techniques to estimate populations and work across larger geographical scales to develop effective management strategies for the conservation of primates in India.

Oman has been recognised as the leading country in terms of recent developments in public health. However, there is a shortage of mental health services, which poses challenges for people seeking such services, particularly those from rural areas. This often results in delayed treatment and longer periods of untreated mental illness.

The Palaeocene–Eocene Thermal Maximum (PETM) was a global extreme climatic event, but it is relatively unknown from lower latitudes or equatorial regions in comparison to mid- and high latitudes. The present study provides the first clay mineralogical evidence of the PETM and subsequent hyperthermal events in a near-equatorial region represented by the Akli Formation in the Barmer Basin, India. The 32 m-thick succession of the Akli Formation shows abrupt changes in smectite and kaolin abundances preceding, during and succeeding the PETM event. Within the studied section, the kaolin content increases from 5–8% pre-PETM to 30–35% during the PETM, and then again decreases to 5–6% during the post-PETM period. The smectite, however, is marked by a corresponding decrease and its transformation into kaolin in acid weathering conditions. The transformation of the smectite is first marked by hydroxy interlayering and then transformation into kaolin during the PETM. The transformation of smectite into kaolin also resulted in extensive precipitation of iron oxide in sediments. The clay mineralogical changes in the Palaeocene–Eocene transition sediments of the Akli Formation were caused by 3–5°C warming and a 25–50% increase in rainfall during the hyperthermal events. Unusually high charcoal (~20%) fragments during the Palaeocene–Eocene transition also suggest warming and widespread biomass burning during the PETM in the lower latitudes.