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Timed artificial insemination plus heat I: effect of estrus expression scores on pregnancy of cows subjected to progesterone–estradiol-based protocols

Published online by Cambridge University Press:  15 April 2019

E. Nogueira Open the ORCID record for E. Nogueira [Opens in a new window] ,
M. R. Silva ,
J. C. B. Silva ,
U. P. G. Abreu ,
N. A. Anache ,
K. C. Silva ,
C. J. T. Cardoso ,
P. Sutovsky  and
W. B. Rodrigues
E. Nogueira*
Affiliation:
Embrapa Pantanal, Rua 21 de Setembro 1880, Corumbá, MS 79320-900, Brasil Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
M. R. Silva
Affiliation:
Melhore Animal Consultoria LTDA, Rua Glycia Pedroso Fenerich, 147 box 06, Jaboticabal- SP, 14871-835, Brasil
J. C. B. Silva
Affiliation:
Embrapa Pantanal, Rua 21 de Setembro 1880, Corumbá, MS 79320-900, Brasil
U. P. G. Abreu
Affiliation:
Embrapa Pantanal, Rua 21 de Setembro 1880, Corumbá, MS 79320-900, Brasil
N. A. Anache
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
K. C. Silva
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
C. J. T. Cardoso
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias, UFMS – Av. Senador Filinto Muller, 2443, Campo Grande -MS, 79074-460, Brasil
P. Sutovsky
Affiliation:
Department of Obstetrics, Gynecology & Women’s Health, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
W. B. Rodrigues
Affiliation:
Melhore Animal Consultoria LTDA, Rua Glycia Pedroso Fenerich, 147 box 06, Jaboticabal- SP, 14871-835, Brasil
*
†E-mail: eriklis.nogueira@embrapa.br
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Abstract

Expression of estrus near timed artificial insemination (TAI) is associated with greater fertility, and estrus detection could improve TAI fertility or direct TAI management, although accurate estrus detection can be difficult and time-consuming using traditional methods. The aim of this study is to evaluate influence of estrus on pregnancy (artificial insemination pregnancy rates (P/AI)) and to validate an alternative method to classify estrus/heat expression using tail chalking (HEATSC) in postpartum Bos indicus cows subjected to TAI in progesterone–estrogen-based protocols. In experiment 1 (Exp. 1), cows (5491) were subjected to visual observation of estrus after progesterone device removal, before TAI, and P/AI was evaluated according to estrus and body condition score (BCS). Cows received a progesterone device and 2 mg estradiol benzoate (EB). After 8 days, the device was removed and 150 μg of d-cloprostenol and 300 IU equine chorionic gonadotrophin was given. Later, animals in Exp. 1 received 1 mg EB and TAI 44 to 48 h. In the Exp. 2 – 3830 cows using similar protocol, received different ovulation inducers: 1 mg EB (n=1624) or 1 mg estradiol cypionate (EC; n=2206) on day 8 (D8). Cows were then marked with chalk, and HEATSC evaluated at TAI on D10 (HEATSC1 – no chalk removal=no estrus expression; HEATSC2 – partial chalk removal=low estrus expression; HEATSC3 – near complete/complete chalk removal=high estrus expression). In Exp. 1, cows showing estrus presented greater P/AI (48.4% v. 40.2%, P<0.05). In Exp. 2, P/AI (HEATSC1 – 40.0%; HEATSC2 – 49.7%; HEATSC3 – 60.9%; P<0.001), and larger follicle timed artificial insemination (LFTAI) (<0.001) varied according to HEATSC. There was no difference in P/AI (P=0.41) or LFTAI (P=0.33) according to ovulation inducer. Cows with greater BCS showed greater P/AI in both experiments (P<0.05). Estrus presence and greater HEATSC improved P/AI, and EC v. EB used promoted differential estrus manifestation (cows showing HEATSC2 and HEATSC3: 79.5% with EB v. 69.98% with EC use, P<0.001), however, with similar P/AI. The use of HEATSC in B. indicus cows subjected to TAI is useful to identify cows with greater estrus expression and consequently improved pregnancy rates in TAI, allowing the cows with low HEATSC to be targeted for additional treatments aimed at improving P/AI.

Keywords

Bos indicusartificial inseminationfolliclereproductionheat score
Type
Research Article
Information
animal , Volume 13 , Issue 10 , October 2019 , pp. 2305 - 2312
Copyright
© The Animal Consortium 2019 

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