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Voltage-clamp analysis and computational model of dopaminergic neurons from mouse retina

Published online by Cambridge University Press:  25 February 2005

JIANGUO XIAO ,
YIDAO CAI ,
JASPER YEN ,
MICHAEL STEFFEN ,
DOUGLAS A. BAXTER ,
ANDREAS FEIGENSPAN  and
DAVID MARSHAK
JIANGUO XIAO
Affiliation:
Department of Neurobiology and Anatomy, The University of Texas—Houston Medical School, Houston
YIDAO CAI
Affiliation:
Department of Neurobiology and Anatomy, The University of Texas—Houston Medical School, Houston
JASPER YEN
Affiliation:
Department of Neurobiology and Anatomy, The University of Texas—Houston Medical School, Houston
MICHAEL STEFFEN
Affiliation:
Department of Neurobiology and Anatomy, The University of Texas—Houston Medical School, Houston
DOUGLAS A. BAXTER
Affiliation:
Department of Neurobiology and Anatomy, The University of Texas—Houston Medical School, Houston
ANDREAS FEIGENSPAN
Affiliation:
Department of Neurobiology, University of Oldenburg, Oldenburg, Germany
DAVID MARSHAK
Affiliation:
Department of Neurobiology and Anatomy, The University of Texas—Houston Medical School, Houston
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Abstract

Isolated dopaminergic amacrine (DA) cells in mouse retina fire rhythmic, spontaneous action potentials and respond to depolarizing current with trains of low-frequency action potentials. To investigate the roles of voltage-gated ion channels in these processes, the transient A-type K+ current (IK,A) and Ca2+ current (ICa) in isolated mouse DA cells were analyzed by voltage clamp. The IK,A activated at −60 mV and inactivated rapidly. ICa activated at around −30 mV and reached a peak at 10 mV without apparent inactivation. We also extended our previous computational model of the mouse DA cell to include the new electrophysiological data. The model consisted of a membrane capacitance in parallel with eight currents: Na+ transient (INa,T), Na+ persistent (INa,P), delayed rectifier potassium (IKdr), IK,A, calcium-dependent potassium (IK,Ca), L-type Ca2+ ICa, hyperpolarization-activated cation current (Ih), and a leak current (IL). Hodgkin-Huxley type equations were used to define the voltage- and time-dependent activation and inactivation. The simulations were implemented using the neurosimulator SNNAP. The model DA cell was spontaneously active from a wide range of initial membrane potentials. The spontaneous action potentials reached 35 mV at the peak and hyperpolarized to −76 mV between spikes. The spontaneous firing frequency in the model was 6 Hz. The model DA cell responded to prolonged depolarizing current injection by increasing its spiking frequency and eventually reaching a depolarization block at membrane potentials greater than −10 mV. The most important current for determining the firing rate was IK,A. When the amplitude of IK,A was decreased, the firing rate increased. ICa and IK,Ca also affected the width of action potentials but had only minor effects on the firing rate. Ih affected the firing rate slightly but did not change the waveform of the action potentials.

Keywords

Calcium currentPotassium currentIon channelAmacrine cellVoltage-dependent conductance
Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 6 , November 2004 , pp. 835 - 849
Copyright
© 2004 Cambridge University Press

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