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TcNST2 encodes a Golgi-localized UDP-galactose transporter in Trypanosoma cruzi

Published online by Cambridge University Press:  21 June 2019

Elizabeth C. Rodrigues ,
Patricia Mörking ,
Jaqueline O. Rosa ,
Bruno A. A. Romagnoli ,
Beatriz G. Guimarães ,
Priscila M. Hiraiwa ,
Amanda Klinke ,
Alessandra M. de Aguiar ,
Crisciele Kuligovski  and
Samuel Goldenberg
...Show all authors
Elizabeth C. Rodrigues
Affiliation:
Departament of Immunology, Biomedical Sciences Institute, University of São Paulo, São Paulo, São Paulo 05508-900, SP, Brazil
Patricia Mörking
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Jaqueline O. Rosa
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Bruno A. A. Romagnoli
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Beatriz G. Guimarães
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Priscila M. Hiraiwa
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Amanda Klinke
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Alessandra M. de Aguiar
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Crisciele Kuligovski
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Samuel Goldenberg
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
Augusto S. P. Ramos*
Affiliation:
Institute Carlos Chagas, Fiocruz Paraná, Curitiba 81350-010, PR, Brasil
*
Author for correspondence: Augusto S. P. Ramos, E-mail: augusto.ramos@fiocruz.br
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Abstract

Survival and infectivity of trypanosomatids rely on cell-surface and secreted glycoconjugates, many of which contain a variable number of galactose residues. Incorporation of galactose to proteins and lipids occurs along the secretory pathway from UDP-galactose (UDP-Gal). Before being used in glycosylation reactions, however, this activated sugar donor must first be transported across the endoplasmic reticulum and Golgi membranes by a specific nucleotide sugar transporter (NST). In this study, we identified an UDP-Gal transporter (named TcNST2 and encoded by the TcCLB.504085.60 gene) from Trypanosoma cruzi, the etiological agent of Chagas disease. TcNST2 was identified by heterologous expression of selected putative nucleotide sugar transporters in a mutant Chinese Hamster Ovary cell line. TcNST2 mRNA levels were detected in all T. cruzi life-cycle forms, with an increase in expression in axenic amastigotes. Confocal microscope analysis indicated that the transporter is specifically localized to the Golgi apparatus. A three-dimensional model of TcNST2 suggested an overall structural conservation as compared with members of the metabolite transporter superfamily and also suggested specific features that could be related to its activity. The identification of this transporter is an important step toward a better understanding of glycoconjugate biosynthesis and the role NSTs play in this process in trypanosomatids.

Keywords

Glycoconjugatenucleotide sugartransportertrypanosomatids
Type
Research Article
Information
Parasitology , Volume 146 , Issue 11 , September 2019 , pp. 1379 - 1386
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Copyright
Copyright © Cambridge University Press 2019 

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