Populations of rigid ryegrass with resistance to glyphosate have started to become a problem on fence lines of cropping fields of southern Australian farms. Seed of rigid ryegrass plants that survived glyphosate application were collected from two fence line locations in Clare, South Australia. Dose–response experiments confirmed resistance of these fence line populations to glyphosate. Both populations required 9- to 15-fold higher glyphosate dose to achieve 50% mortality in comparison to a standard susceptible population. The mechanism of resistance in these populations was investigated. Sequencing a conserved region of the gene encoding 5-enolpyruvyl-shikimate-3-phosphate synthase identified no differences between the resistant and susceptible populations. Absorption of glyphosate into leaves of the resistant populations was not different from the susceptible population. However, the resistant plants retained significantly more herbicide in the treated leaf blades than did the susceptible plants. Conversely, susceptible plants translocated significantly more herbicide to the leaf sheaths and untreated leaves than the resistant plants. The differences in translocation pattern for glyphosate between the resistant and susceptible populations of rigid ryegrass suggest resistance is associated with altered translocation of glyphosate in the fence line populations.

Dithiopyr provides PRE and early POST control of smooth crabgrass, but POST efficacy is often inconsistent on tillered plants. Experiments were conducted to evaluate the interaction of temperature and growth stage on dithiopyr efficacy, absorption, translocation, and metabolism in smooth crabgrass. In greenhouse experiments, I50 (predicted rate to induce 50% injury) measured < 0.14, 0.14, and 0.15 kg ha−1 at low temperatures (average 23 C) for multi-leaf, one-tiller, and multi-tiller smooth crabgrass, respectively, while I50 measured < 0.14, 0.88, and > 2.24 kg ha−1 at high temperatures (average 32 C), respectively. Multi-tiller (three to five tillers) smooth crabgrass absorbed more root applied 14C-dithiopyr than multi-leaf (three to four leaves) and one-tiller plants, but specific radioactivity (Bq mg−1) was two to three times greater in multi-leaf plants compared to tillered plants. Smooth crabgrass treated at 15/10 C (day/night) had ≈ two times greater specific radioactivity following root applied 14C-dithiopyr than at 30/25 C. Radioactivity distribution to shoots from root applications measured 43, 30, and 20% of the total absorbed for multi-leaf, one-tiller, and multi-tiller plants, respectively. Smooth crabgrass had two times more foliar absorption of 14C-dithiopyr at 15/10 than 30/25 C while 14C losses were greater at 30/25 than 15/10 C. Smooth crabgrass metabolism of 14C-dithiopyr was ≈ two times greater at 30/25 than 15/10 C, and multi-leaf plants averaged 10 to 20% more metabolism than tillered plants at 7 d after treatment. Results suggest differential absorption, translocation, and metabolism may contribute to dithiopyr efficacy on smooth crabgrass at various growth stages, but use under high temperatures (30/25 C) could increase losses from volatilization, reduce foliar absorption, and increase metabolism compared to cooler temperatures (15/10 C).

One method that appears promising for the treatment of Eurasian watermilfoil in areas of high water exchange is the use of herbicide-impregnated granules. Experiments were conducted using liquid triclopyr-triethylamine and granules impregnated with triclopyr-triethylamine to test this theory. Uniform, multistemmed Eurasian watermilfoil plants were selected for these experiments. Plants were treated in clear acrylic cylinders containing 7 L of water with 0.5 mg/L triclopyr as the liquid triethylamine plus 20 kBq 14C-triclopyr or blank granules impregnated with triclopyr triethylamine plus 20 kBq of 14C-triclopyr. Plants were harvested 6, 12, 24, 48, 96, and 192 h after treatment (HAT) and the radioactivity in the apical meristems, remaining shoot and root was determined with sample oxidation and liquid scintillation spectroscopy. There were no significant differences in overall herbicide absorption by Eurasian watermilfoil following liquid and granular triclopyr treatments; however, differences were observed between plant parts. Apical meristems accumulated the most radioactivity, whereas roots accumulated very little radioactivity following liquid treatment. Granular applications resulted in 7.5 times more radioactivity in the Eurasian watermilfoil roots then the liquid triclopyr application; therefore, long-term control of well-established Eurasian watermilfoil plants could improve with granular applications, especially in areas where rapid herbicide dilution could be an issue.

Since its discovery in 2005, glyphosate-resistant Palmer amaranth has become a major problem for many farmers in the southern United States. One mechanism of resistance found in a Georgia population of glyphosate-resistant Palmer amaranth is amplification of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene throughout the genome, with some resistant plants containing and expressing more than 100 EPSPS genes. Such high numbers of EPSPS genes and protein production could result in a fitness cost to resistant plants due to (1) metabolic cost of overproduction of this enzyme and (2) disruption of other genes after insertion of the EPSPS gene. A greenhouse experiment was set up to investigate differences in growth and reproduction between glyphosate-susceptible and -resistant Palmer amaranth plants. Measurements included growth rate, plant height/volume ratio, final biomass, photosynthetic rate, inflorescence length, pollen viability, and seed set. This study found no significant fitness costs for plants with the resistance trait. This study also provided a clear example of how controlling for genetic background is important in fitness cost studies and how potentially misleading results can be obtained if only a few fitness traits are measured. These results indicate that glyphosate-resistant Palmer amaranth plants with high EPSPS gene copy numbers are likely to persist in field populations, even in the absence of glyphosate, potentially leading to long-term loss of glyphosate as a control option for Palmer amaranth.

Our objective was to evaluate the cost/benefit of a single herbicide application or targeted grazing of invasive annual grasses during restoration of partially invaded sagebrush steppe ecosystems used for livestock production. The cost/benefit model used is based on estimating the production of vegetation in response to implementing management and modeling cost/benefit economics associated with that prediction. The after-tax present value of added animal unit months (AUMs) obtained was lower than the present value of after-tax treatment costs after 20 yr for a single herbicide treatment, but higher than the present value of after-tax treatment costs for the grazing management scenario. Even at the highest weed utilization level, the value of added AUMs did not offset the cost of the treatment after 20 yr. However, the grazing treatment resulted in a value of added AUMs higher than the costs after 20 yr. Depending on the invasive weed utilization level, break-even points with targeted grazing occurred at anywhere from the first year to 7 yr. This assessment clearly shows that grazing management can be economically viable for managing annual grass-infested rangeland. In the future, models like the one used here can be improved by incorporating the rangeland management and restoration benefits on the wide variety of goods and services gained from rangeland.

Citron melon is a monoecious, hairy annual vine commonly found in citrus orchards, and cotton and peanut fields. Information is not available on the effect of various environmental factors on the germination of citron melon. Laboratory and greenhouse experiments were carried out in 2011 and 2012 to determine the effect of light, temperature, salinity, pH, simulated water stress, and depth of sowing on the germination of citron melon. Citron melon germination was affected by various environmental factors. Highest germination was observed at day/night temperatures of 25/20 to 30/25 C regardless of light conditions. At temperatures below 25 C and beyond 35 C, germination declined and was higher under dark condition than light. Germination decreased as osmotic potential became more negative (−0.3 MPa to −1.5 MPa) and salt concentration increased (50 to 350 mM). No germination was observed at > −0.9 MPa and ≥ 300 mM salt concentrations. However, germination was observed over a broad range of pH (3 to 9) and up to 10-cm sowing depths. Seeds sown at the surface did not germinate but maximum germination (88 to 96%) occurred at 2- to 4-cm depth. The results of this study suggest that citron melon can grow in a wide range of climatic conditions and therefore can persist in Florida because of favorable weather and environmental conditions.

During 2004 to 2008, weed surveys were conducted in 373 wheat fields of two different cropped areas (southwest [SW] and southeast [SE]) of the southern region of Buenos Aires Province of Argentina where different weed communities were expected because of changes in cropping practices over time, including tillage, crop sequence, fertilizers, and herbicides applied. Weed communities differed between regions, with greater numbers of native species for the SW. Weed community diversity was also greater for the SW region, probably due to the more diverse land use that resulted in greater landscape heterogeneity. Rush skeletonweed, sand rocket, yellow starthistle and turnipseed occurred at higher constancy (proportion of fields in which a given species is present) in the SW region, whereas common chickweed, false bishop's weed, corn speedwell, and common lambsquarters were present more frequently in the SE region. Compared with the 1982 survey, constancy of weeds increased, but those species with high constancy in 1982 were also with high constancy in the recent surveys. Diversity (species richness) was greater in conventional than in a no-tillage system. The constancy of Italian ryegrass, sand rocket, and yellow starthistle was lower under no-till than conventional tillage. Surveys allow identification of changes in weed community related to different agricultural systems. Rotation of crops and livestock avoid the homogenization of the environment at the landscape level. Management strategies will be necessary to prevent the increase of weeds populations' size, preserving plant diversity and the properties of the agroecosystem.

Predictions of weed emergence can be used by practitioners to schedule POST weed management operations. Common sunflower seed from Kansas was used at six Midwestern U.S. sites to examine the variability that 16 climates had on common sunflower emergence. Nonlinear mixed effects models, using a flexible sigmoidal Weibull function that included thermal time, hydrothermal time, and a modified hydrothermal time (with accumulation starting from January 1 of each year), were developed to describe the emergence data. An iterative method was used to select an optimal base temperature (Tb) and base and ceiling soil matric potentials (ψb and ψc) that resulted in a best-fit regional model. The most parsimonious model, based on Akaike's information criterion (AIC), resulted when Tb = 4.4 C, and ψb = −20000 kPa. Deviations among model fits for individual site years indicated a negative relationship (r = −0.75; P < 0.001) between the duration of seedling emergence and growing degree days (Tb = 10 C) from October (fall planting) to March. Thus, seeds exposed to warmer conditions from fall burial to spring emergence had longer emergence periods.

Greenhouse and laboratory experiments were conducted on common waterhemp and soil collected from 131 soybean fields in Missouri that contained late-season common waterhemp escapes. The objectives of these experiments were to determine the effects of soil sterilization on glyphosate-resistant (GR) and -susceptible (GS) common waterhemp survival, to determine the effects of soil sterilization and glyphosate treatment on infection of GR and GS common waterhemp biotypes by Fusarium spp., and to determine the soil microbial abundance and diversity in soils collected from soybean fields with differences in common waterhemp biotypes and herbicide and crop rotation histories. Common waterhemp biotypes were treated with 1.7 kg glyphosate ae ha−1 or left untreated once plants reached approximately 15 cm in height. Common waterhemp survival was visually assessed at 21 d after glyphosate treatment (21 DAT). To determine Fusarium infection frequency, a single intact common waterhemp root was harvested from each treatment at 0, 3, 7, 14, and 21 DAT and surface sterilized, and 10 to 15–mm common waterhemp root sections were plated on Komada culture medium. After 14 d incubation, fungal colonies were selected from colonized roots and maintained on potato dextrose agar medium amended with antibiotics before identification. Speciation of Fusarium isolates was conducted through microscopic examination of fungal characters and confirmed by sequencing and analysis of ribosomal DNA. Soil samples from 131 different collections were subjected to phospholipid fatty acid (PLFA) analysis and were conducted utilizing gas chromatography to determine the soil microbial community abundance and structure. Common waterhemp plants grown in sterile soils had the highest common waterhemp survival, regardless of biotype. After treatment with glyphosate, survival of GS common waterhemp grown in nonsterile soil was only 29% 21 DAT, whereas survival of GS common waterhemp grown in nonsterile soil was only 10%. Similarly, GR common waterhemp survival was reduced from 83 to 61% following treatment with glyphosate when grown in nonsterile compared to sterile soil. Fusarium spp. were recovered from only 12% of the assayed roots (223 treatments with Fusarium out of a total 1,920 treatments). The greatest occurrence of Fusarium root infection in both GR and GS common waterhemp occurred in nonsterile soils following a glyphosate treatment. Few differences in total PLFA were observed in field soil collected from locations with either GR or GS common waterhemp, and regardless of herbicide or crop history. This research supports previous findings that plant species are more sensitive to glyphosate in nonsterile than sterile soils and indicates glyphosate may predispose plants to soil-borne phytopathogens. This research also suggests that continuous use of glyphosate does not significantly affect soil microbial abundance or diversity.

Winter annual weeds are becoming prolific in agricultural fields in the midwestern United States. The objectives of this research were to understand the roles of soil temperature (daily average and fluctuation) and moisture on the emergence of nine winter annual weed species and dandelion and to develop predictive models for weed emergence based on the accumulation of modified thermal/hydrothermal time (mHTT). Experiments were established at Lincoln, NE; Mead, NE; and at two sites (irrigated and rainfed) near Clay Center, NE, in 2010 and 2011. In July of each year, 1,000 seeds of each species were planted in 15 by 20 by 6-cm mesh baskets installed between soybean rows. Soil temperature and water content were recorded at the 2-cm depth. Emerged seedlings were counted and removed from the baskets on a weekly basis until no additional emergence was observed in the fall, resumed in late winter, and continued until emergence ceased in late spring. Weather data were used to accumulate mHTT beginning on August 1. A Weibull function was selected to fit cumulative emergence (%) on cumulative mHTT (seven base temperature [Tbase] by six base water potential [Ψbase] by three base temperature fluctuation [Fbase] candidate threshold values = 126 models); it was also fit to days after August 1 (DAA1), for a total of 127 candidate models per species. The search for optimal base thresholds was based on the theoretic-model comparison approach (Akaike information criterion [AIC]). All three components (Tbase, Ψbase, and Fbase) were only important for Virginia pepperweed. For downy brome and purslane speedwell, including Tbase and Ψbase resulted in the best fit, whereas for dandelion including Tbase and Fbase resulted in the best fit. A model including only Tbase resulted in the best fit for most species included in this study (Carolina foxtail, shepherd's-purse, pinnate tansymustard, henbit, and field pansy). For field pennycress, the model based on DAA1 resulted in the best fit. Threshold values were species specific. Soil temperature was the major environmental factor influencing winter annual weed emergence. Even though soil moisture and often temperature fluctuation are essential for seed germination, Ψbase and Fbase were not as critical in the predictive models as initially expected. Most seedlings (> 90%) of downy brome, pinnate tansymustard, Carolina foxtail, henbit, and field pansy emerged during the fall. Virginia pepperweed, purslane speedwell, dandelion, shepherd's-purse, and field pennycress seedlings emerged during both fall and spring. The results of this research provide robust information on the prediction of the time of winter annual weed emergence, which can help growers make better management decisions.

Winter annual weeds protect the soil from erosion and retain nutrients during the winter; however, they can also act as a host for crop pests and pathogens and impede planting. Increased knowledge of the reproductive biology and the seed fate of winter annuals would be useful to improve management and crop productivity. The objectives of this research were to determine the recruitment biology of shepherd's-purse, henbit, common chickweed, and field pennycress, including seed production, dispersal, dormancy, and seedling emergence, based on growing degree days (GDD). Henbit was the least prolific of the four weeds studied, producing 800 to 40,000 seeds m−2 at naturally occurring densities; shepherd's-purse was the most prolific, producing 11,000 to 400,000 seeds m−2 with 40 to 230 plants m−2. Fifty percent seed rain occurred for henbit, common chickweed, shepherd's-purse, and field pennycress at 620, 790, 880, and 1300 GDDBase,0C, respectively. Overall, seeds were dormant for all species at the time of dispersal. In 2 of 3 yr, dormancy of later-dispersed common chickweed decreased after 6 mo of storage at natural, fluctuating temperatures in the absence of water. The emergence patterns of the four species followed the Gompertz equation and were indicative of facultative winter annuals. The emergence patterns by rate were similar between henbit and common chickweed and between shepherd's-purse and field pennycress. Seed production, dispersal, dormancy, and seedling emergence were influenced by moisture; therefore, including a precipitation or soil moisture component into a GDD model (such as the use of hydrothermal time) would improve the accuracy of predicting winter annual reproduction, seed fate, and emergence.

Although the prolonged emergence pattern of common waterhemp is well known, its effect on flowering phenology and success is less well understood. The ecological significance of later common waterhemp cohorts could have been underestimated. Ecological knowledge of common waterhemp, such as reproductive phenology and success, would help us better understand the invasiveness of this weed and thus facilitate the development of more targeted control methods. Field studies were conducted at Ames, IA, to evaluate temporal variation in flowering phenology (e.g., date of flower initiation, number of flowering plants per day) and reproductive success (e.g., seed production) of natural common waterhemp cohorts. Later-emerging common waterhemp cohorts flowered quicker and had a relatively shorter flowering period than early cohorts. Common waterhemp cohorts showed very large variations for the duration of their vegetative phases, indicating different photoperiod sensitivity among cohorts. Furthermore, common waterhemp cohorts exhibited a pulsed flowering pattern, which was probably influenced by temporal distribution of rain events; up to seven distinct flowering pulses within 40 d were observed in 2009, and about eight flowering pulses scattered over a 60 d period were observed in 2010. Common waterhemp maintained high seed production throughout the growing season. Seed production for the entire cohort was influenced more by emergence timing, whereas individual plant fecundity was more affected by plant population densities. Common waterhemp demonstrates strong phenological plasticity by evolving a pulsed flowering pattern, which rendered it plastic enough to tailor flowering to variable environmental conditions thus facilitating effective pollination. Common waterhemp later cohorts have a strong seed production potential, which may be ecologically significant with regard to herbicide resistance evolution.

Yellow rattle is a hemiparasitic weed that is becoming increasingly problematic in hay fields in the northeastern United States. The biomass of yellow rattle was assessed in two experiments in which commercially available soil amendments were applied to an infested hay field under no-till management. The amendments were applied in the fall of 2010 and 2011 and included wood ash, biochar, hardwood sawdust, lime, and K. Wood ash applied at a rate of 8,967 kg ha−1 (4 tons per acre) had the greatest suppressive effect and reduced yellow rattle biomass the following summer by 86 and 92% relative to the untreated control in Experiments 1 and 2, respectively. Two additional treatments included in Experiment 2, a doubled rate of wood ash and sawdust applied at a rate of 13,450 kg ha−1, also reduced yellow rattle biomass. No other treatments affected yellow rattle biomass. A partial least-squares regression (PLSR) analysis that included aboveground plant community and soil biochemical data explained only 42.4% of the variation in yellow rattle biomass, suggesting that the mechanisms responsible for the suppression of yellow rattle by wood ash and sawdust are complex. Amending hay fields with wood ash or sawdust may be an effective nonherbicide strategy for managing yellow rattle infestations. Additional research will be required to determine the mechanisms and generality of yellow rattle suppression by wood ash and sawdust amendments to the soil.

Grower adoption of no-tillage (NT) approaches to organic soybean production has been limited, in part because of the perceived risks of ineffective cover crop management and lack of season-long weed suppression. We conducted research in 2008 and 2009 to assess those risks by quantifying the effects of winter rye cover-crop management (tilling, crimping, or mowing), soybean planting date (mid May or early June), and row width (19 or 76 cm) on weed recruitment, emergence patterns, season-long suppression, and late-season weed community composition in transitional organic production systems. The weed plant community consisted largely of summer annual species in each year, with velvetleaf or common lambsquarters as the most abundant species. Seedling recruitment from the soil seedbank varied between years, but velvetleaf recruitment was consistently greater in the tilled rye than in the NT rye treatments. Weed emergence tended to peak early in the season in the tilled rye treatment, but in the NT rye treatments, the peak occurred in mid or late season. More-diverse summer annual and perennial species were associated with the NT rye treatments. Even so, weed suppression (as measured by late-season weed shoot mass) was much greater in crimped or mowed rye NT treatments than it was in the tilled treatment. Weed suppression among NT rye treatments was greater in 19- than in 76-row spacing treatments in each year and was greater for mid May than it was for early June planted soybean in 2009. The NT planting of soybean into standing rye before termination (crimping or mowing) facilitated timely planting of soybean, as well as effective, season-long weed suppression, suggesting that those approaches to rye and weed management are of less risk than those typically perceived by growers. Our results suggest that NT systems in winter rye provide effective weed-management alternatives to the typical tillage-intensive approach for organic soybean production.

Prodiamine is a mitotic inhibiting herbicide regularly used to control annual bluegrass PRE. A population of annual bluegrass not controlled by prodiamine at 1,120 g a.i. ha−1 was identified on a golf course in Alcoa, TN, in 2012. A whole-plant hydroponics bioassay was used to screen this biotype for prodiamine resistance (PR) compared with a known susceptible population (SS). Multitiller (i.e., > 4 tillers) PR and SS annual bluegrass plants were established in hydroponic culture and exposed to 0, 0.001, 0.01, 0.10, 1.0, and 10.0 mM prodiamine. Exposure to prodiamine at 0.001 mM reduced root growth of the SS biotype to 26% of the nontreated check (i.e., 0 mM prodiamine) but had no effect on the PR biotype. When exposed to 10 mM prodiamine, root growth of the PR biotype was reduced to 24% of the nontreated check compared with 9% for the SS biotype. I50 values for the PR and SS biotypes were 0.04 and 2.8 × 10−6 mM prodiamine, respectively. The PR biotype measured lower in plant height and leaf width than the SS population. In field trials, prodiamine at 560, 840, 1,120, and 1,400 g ha−1 only controlled the PR biotype 0 to 22%. PRE applications of the cellulose biosynthesis inhibitor indaziflam at 35, 52.5, and 70 g a.i. ha−1 controlled this PR biotype 70 to 97%. This marks the second instance of annual bluegrass developing resistance to prodiamine in Tennessee during the past 5 yr. Future research should evaluate indaziflam efficacy for control of other prodiamine-resistant biotypes of annual bluegrass as well as annual bluegrass biotypes resistant to herbicidal inhibitors of 5-enolpyruvylshikimic acid-3-phosphate synthase, acetolactate synthase, and photosystem II.

Given the importance of emergence level and timing to the competitiveness and success of annual crabgrass species in turfgrass, particularly in the context of increasing synthetic pesticide bans and the common cultural practice of fertilization, a study was conducted in a northern region of North America (Ontario, Canada) to determine the effect of fertilizer application on large and smooth crabgrass emergence in residential lawns. In petri dish experiments, we reconfirmed that KNO3 has a significant positive effect on large and smooth crabgrass seed germination but we showed that there is only an effect on fresh seed and no effect on aged seed, suggesting that the treatment affects dormancy level and not germination per se. In two other experiments using turf cores and commercial lawn fertilizer in growth room conditions and in field trials at three sites, we confirmed this result showing that neither fall nor spring fertilizer application had any effect on the emergence level of either smooth or large crabgrass. These results have practical relevance to homeowners and turf managers in this region because they are dealing with crabgrass emerging in the spring from seed shed the previous fall. The results also show that fertilizer can be used to aid turf quality and competitiveness without impacting true infestation level (density) of crabgrass in the spring.

Kochia is a weed found in many sunflower fields across the Northern Great Plains. There is limited information about the ability of sunflower plants to compete with kochia, specifically when the weed grows both in the crop row and in the inter-row space, as in zero tillage systems that rely solely on herbicides to manage weeds. An experiment was conducted over seven site–yr, from 2009 to 2011, to determine the effect of kochia density and relative time of kochia seedling recruitment on sunflower growth and development, yield and seed quality. Kochia seed was broadcast on the soil surface at six densities, into sunflowers planted in 75-cm rows, either at the same time as the sunflower crop was planted (early weed seedling recruitment), or when the sunflowers were at the four-leaf stage (late weed seedling recruitment). When kochia plants emerged at the same time as the sunflowers, yield was reduced by up to 76% and sunflower head diam was reduced in four site–yr, stem diam was reduced in three site–yr, height was reduced in two site–yr and the number of leaves per sunflower plant was reduced in two site–yr The 5% action threshold for early emerging kochia was four kochia plants m−2 in the combined site–yr analysis. Additionally, early recruiting kochia seedlings reduced sunflower seed size and seed weight at two and three site–yr, respectively. Kochia plants that emerged after the four-leaf stage of the sunflower crop did not affect sunflower growth and development, yield, or seed quality. To reduce the potential for yield and seed quality losses, sunflower growers should be proactive with respect to managing kochia in sunflowers, particularly when the kochia plants emerge at about the same time as the sunflowers.

Crop rotation has long been considered one of the simplest and most effective tools for managing weeds. In this paper, we demonstrate how crop rotations can be strategically arranged to harness a novel mechanism of weed suppression: weed-weed competition. Specifically, we consider how crop stacking, or increasing the number of consecutive plantings of a single crop within a rotation, can decrease the size of the weed seed bank, by forcing weeds to compete with each other in similar environments for longer periods of time, while still reaping the traditional benefits of crop rotation. Using an annual plant model, we investigate the theoretical effects of stacked crop rotations on weeds that have different life-history strategies and phenology. Our results show that when weeds compete within a season, stacking can reduce the weed seed bank compared to rotations without stacked crops. Although more research is needed to fully understand the effects of crop stacking on other aspects of the system, such as insect pests and diseases, our research suggests that crop stacking has the potential to improve weed suppression without additional inputs, and their associated costs and externalities. More generally, improving management by changing the temporal arrangement of disturbances is a novel, process-based approach that could likely be applied to other weed management practices, such as mowing and herbicide application, and which could involve mechanisms other than weed-weed competition. Leveraging this new application of existing ecological theory to improve weed management strategies holds great promise.

The implementation of site-specific weed management requires information about weed cover and decision support systems to determine weed cover thresholds and concomitant herbicide rates. Although it is possible to create accurate weed cover maps over large areas, weed cover thresholds have generally been evaluated using tedious weed density counts. To bridge this gap between weed cover obtained by machine vision and the concept of economic threshold, crop advisers specializing in weed scouting were asked to evaluate over 2,500 weed cover images (2 m by 3 m) and determine if a given image would require herbicide application or not. Using the area under the “receiver operating characteristic” curve method, an optimal weed cover threshold was established. The derived economic thresholds ranged from 0.06 to 0.31% weed cover contingent on the level of tolerance of the expert adviser. Although this threshold seems low, it is comparable with economic threshold values based on weed density.