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A new set of microsatellite loci for Cattleya walkeriana Gardner, an endangered tropical orchid species and its transferability to Cattleya loddigesii Lindl. and Cattleya nobilior Reichenbach

Published online by Cambridge University Press:  20 July 2017

Bruno C. Rossini ,
Mariá B. Kampa ,
Celso L. Marino ,
Fernanda Bortolanza Pereira Open the ORCID record for Fernanda Bortolanza Pereira [Opens in a new window] ,
Fábio M. Alves ,
Anete Pereira de Souza ,
Bruna Ibanes ,
Kaiser Dias Schwarcz ,
Maria Imaculada Zucchi  and
Rogério Bobrowski
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Bruno C. Rossini
Affiliation:
Instituto de Biotecnologia (IBTEC), UNESP – Universidade Estadual Paulista, 18607-440 Botucatu, SP, Brazil Departamento de Genética, UNESP – Universidade Estadual Paulista, 18618-689 Botucatu, SP, Brazil
Mariá B. Kampa
Affiliation:
Universidade Estadual do Centro-Oeste (UNICENTRO), PR 153, Km 7, Irati, PR 84500-000, Brazil
Celso L. Marino
Affiliation:
Instituto de Biotecnologia (IBTEC), UNESP – Universidade Estadual Paulista, 18607-440 Botucatu, SP, Brazil Departamento de Genética, UNESP – Universidade Estadual Paulista, 18618-689 Botucatu, SP, Brazil
Fernanda Bortolanza Pereira
Affiliation:
Programa de Pós-Graduação em Ciência Florestal (FCA/UNESP) – Rua José Barbosa de Barros, 1780, Portaria II: Rodovia Alcides Soares, Km 3, 18610-307, Botucatu, SP, Brazil
Fábio M. Alves
Affiliation:
Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, CP 6109, Campinas, SP, CEP 13083-862, Brazil
Anete Pereira de Souza
Affiliation:
Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, CP 6109, Campinas, SP, CEP 13083-862, Brazil
Bruna Ibanes
Affiliation:
Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo’, Av. Pádua Dias, 11, PO Box 9, Piracicaba, SP 13418-900, Brazil
Kaiser Dias Schwarcz
Affiliation:
Agência Paulista de Tecnologia dos Agronegócios, Pólo Regional Centro Sul, Rodovia SP127, Km 30, CP 28, Piracicaba, SP 13400-970, Brazil
Maria Imaculada Zucchi
Affiliation:
Agência Paulista de Tecnologia dos Agronegócios, Pólo Regional Centro Sul, Rodovia SP127, Km 30, CP 28, Piracicaba, SP 13400-970, Brazil
Rogério Bobrowski
Affiliation:
Universidade Estadual do Centro-Oeste (UNICENTRO), PR 153, Km 7, Irati, PR 84500-000, Brazil
Fabiana S. B. Peres
Affiliation:
Universidade Estadual do Centro-Oeste (UNICENTRO), PR 153, Km 7, Irati, PR 84500-000, Brazil
Evandro Vagner Tambarussi*
Affiliation:
Universidade Estadual do Centro-Oeste (UNICENTRO), PR 153, Km 7, Irati, PR 84500-000, Brazil Programa de Pós-Graduação em Ciência Florestal (FCA/UNESP) – Rua José Barbosa de Barros, 1780, Portaria II: Rodovia Alcides Soares, Km 3, 18610-307, Botucatu, SP, Brazil
*
*Corresponding author. E-mail: tambarussi@gmail.com
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Abstract

In this study, we isolate and analyse a new set of microsatellite loci for Cattleya walkeriana. Twenty-two primer pairs were screened for C. walkeriana (n = 32) and assessed for their transferability to Cattleya loddigesii (n = 12) and Cattleya nobilior (n = 06). All loci amplified for C. walkeriana; however, for C. loddigesii and C. nobilior, four and five primers, respectively, did not present amplification. The polymorphic loci presented between 2 and 13 alleles per locus for both C. walkeriana and C. loddigesii, with respective averages of 5.1 and 4.2. For C. nobilior, we found between two and five alleles per locus, with an average of 2.6. For C. walkeriana, observed heterozygosity varied from 0.100 to 0.966, whereas expected heterozygosity ranged from 0.097 to 0.900. The observed and expected heterozygosity for C. loddigesii and C. nobilior were also estimated. We found no significant linkage disequilibrium between any pair of loci, and evidence of null alleles at four loci (Cw16, Cw24, Cw30 and Cw31) for C. walkeriana. The combined power to exclude the first parent and combined non-exclusion probability of identity were 0.999 and 2.3 × 10−20, respectively. These new loci can be used in studies of germplasm resources, and assessments of genotypic and genetic diversity and population structure, thus improving the accuracy of such analyses and their applicability in the conservation and protection of these endangered species.

Keywords

Brazilian orchidconservation geneticshybridizationpopulation geneticstropical orchid species
Type
Short Communication
Information
Plant Genetic Resources , Volume 16 , Issue 3 , June 2018 , pp. 284 - 287
Copyright
Copyright © NIAB 2017 

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