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Effects of the inclusion of Moringa oleifera seed on rumen fermentation and methane production in a beef cattle diet using the rumen simulation technique (Rusitec)

Published online by Cambridge University Press:  29 June 2018

T. O. J. D’A. Lins ,
S. A. Terry ,
R. R. Silva ,
L. G. R. Pereira ,
L. J. Jancewicz ,
M. L. He ,
Y. Wang ,
T. A. McAllister  and
A. V. Chaves Open the ORCID record for A. V. Chaves [Opens in a new window]
T. O. J. D’A. Lins
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, T1J 4B1 Canada Universidade Estadual do Sudoeste da Bahia, Programa de Pós-graduação em Zootecnia (PPZ/UESB), Praça Primavera, 40, Bairro Primavera, Itapetinga, Bahia, CEP 45700-000, Brazil
S. A. Terry
Affiliation:
School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
R. R. Silva
Affiliation:
Universidade Estadual do Sudoeste da Bahia, Programa de Pós-graduação em Zootecnia (PPZ/UESB), Praça Primavera, 40, Bairro Primavera, Itapetinga, Bahia, CEP 45700-000, Brazil
L. G. R. Pereira
Affiliation:
Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais 70770-901, Brazil
L. J. Jancewicz
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, T1J 4B1 Canada
M. L. He
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, T1J 4B1 Canada
Y. Wang
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, T1J 4B1 Canada
T. A. McAllister
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, T1J 4B1 Canada
A. V. Chaves*
Affiliation:
School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
*
†E-mail: alex.chaves@sydney.edu.au
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Abstract

Moringa oleifera seeds are currently being used as a livestock feed across tropical regions of the world due to its availability and palatability. However, limited knowledge exists on the effects of the raw seeds on ruminant metabolism. As such, the rumen stimulation technique was used to evaluate the effects of substituting increasing concentrations of ground Moringa seeds (0, 100, 200 and 400 g/kg concentrate dry matter (DM)) in the diet on rumen fermentation and methane production. Two identical, Rusitec apparatuses, each with eight fermenters were used with the first 8 days used for adaptation and days 9 to 16 used for measurements. Fermenters were fed a total mixed ration with Urochloa brizantha as the forage. Disappearance of DM, CP, NDF and ADF linearly decreased (P<0.01) with increasing concentrations of Moringa seeds in the diet. Total volatile fatty acid production and the acetate to propionate ratio were also linearly decreased (P<0.01). However, only the 400 g/kg (concentrate DM basis) treatment differed (P<0.01) from the control. Methane production (%), total microbial incorporation of 15N and total production of microbial N linearly decreased (P<0.01) as the inclusion of Moringa seeds increased. Though the inclusion of Moringa seeds in the diet decreased CH4 production, this arose from an unfavourable decrease in diet digestibility and rumen fermentation parameters.

Keywords

MoringatropicsUrochloa brizanthafat supplementationoilseeds
Type
Research Article
Information
animal , Volume 13 , Issue 2 , February 2019 , pp. 283 - 291
Copyright
© The Animal Consortium 2018 

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References

Al_husnan, LA and Alkahtani, MDF 2016. Impact of Moringa aqueous extract on pathogenic bacteria and fungi in vitro. Annals of Agricultural Sciences 61, 247–250.Google Scholar
ANUALPEC 2015. Anuário da Pecuária Brasileira, Instituto FNP and Agra Pesquisas Ltda. São Paulo, Brazil.Google Scholar
Association of Official Analytical Chemists (AOAC) 1990. Official methods of analysis of the AOAC, 15th edition. AOAC, Arlington, VA, USA.Google Scholar
Azad, AK, Rasul, MG, Khan, MMK, Sharma, SC and Islam, R 2015. Prospect of moringa seed oil as a sustainable biodiesel fuel in Australia: a review. Procedia Engineering 105, 601–606.Google Scholar
Beauchemin, KA, McGinn, SM and Petit, HV 2007. Methane abatement strategies for cattle: lipid supplementation of diets. Canadian Journal of Animal Science 87, 431–440.Google Scholar
Berchielli, TT, Messana, JD and Canesin, RC 2012. Produção de metano entérico em pastagens tropicais. Revista Brasileira de Saúde e Produção Animal 13, 954–968.Google Scholar
Brask, M, Lund, P, Hellwing, ALF, Poulsen, M and Weisbjerg, MR 2013. Enteric methane production, digestibility and rumen fermentation in dairy cows fed different forages with and without rapeseed fat supplementation. Animal Feed Science and Technology 184, 67–79.Google Scholar
Busani, M, Patrick, JM, Arnold, H and Voster, M 2011. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African Journal of Biotechnology 10, 12925–12933.Google Scholar
Canadian Council on Animal Care (CCAC) 2009. Guidelines on: The care and use of farm animals in research, teaching and testing. Google Scholar
Carro, MD, Ranilla, MJ, Martín-García, AI and Molina-Alcaide, E 2009. Comparison of microbial fermentation of high- and low-forage diets in Rusitec, single-flow continuous-culture fermenters and sheep rumen. Animal 3, 527–534.Google Scholar
Chaves, AV, He, ML, Yang, WZ, Hristov, AN, McAllister, TA and Benchaar, C 2008. Effects of essential oils on proteolytic, deaminative and methanogenic activities of mixed ruminal bacteria. Canadian Journal of Animal Science 88, 117–122.Google Scholar
Cobellis, G, Trabalza-Marinucci, M, Marcotullio, MC and Yu, Z. 2016. Evaluation of different essential oils in modulating methane and ammonia production, rumen fermentation, and rumen bacteria in vitro. Animal Feed Science and Technology 215, 25–36.Google Scholar
Fraser, GR, Chaves, AV, Wang, Y, McAllister, TA, Beauchemin, KA and Benchaar, C 2007. Assessment of the effects of cinnamon leaf oil on rumen microbial fermentation using two continuous culture systems. Journal of Dairy Science 90, 2315–2328.Google Scholar
Gerber, PJ, Steinfeld, H, Henderson, B, Mottet, A, Opio, C, Dijkman, J, Falcucci, A and Tempio, G 2013. Tackling climate change through livestock – a global assessment of emissions and mitigation opportunities. Food and Agriculture Organization of the United Nations, Rome, Italy.Google Scholar
Giraldo, LA, Ranilla, MJ, Tejido, ML and Carro, MD 2007. Influence of exogenous fibrolytic enzymes and fumarate on methane production, microbial growth and fermentation in Rusitec fermenters. British Journal of Nutrition 98, 753–761.Google Scholar
Hoffmann, EM, Muetzel, S and Becker, K 2003. Effects of Moringa Oleifera seed extract on rumen fermentation in vitro. Archives of Animal Nutrition 57, 65–81.Google Scholar
Johnson, AD 1978. Sample preparation and chemical analysis of vegetation. Commonwealth Bureau of Pastures and Field Crops Bulletin 52, 96–102.Google Scholar
Johnson, KA and Johnson, DE 1995. Methane emissions from cattle. Journal of Animal Science 73, 2483–2492.Google Scholar
Lürling, M and Beekman, W 2010. Anti-cyanobacterial activity of Moringa oleifera seeds. Journal of Applied Phycology 22, 503–510.Google Scholar
Maia, MRG, Chaudhary, LC, Bestwick, CS, Richardson, AJ, McKain, N, Larson, TR, Graham, IA and Wallace, RJ 2010. Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. BMC Microbiology 10, 52.Google Scholar
Makkar, HPS and Becker, K 1998. Moringa oleifera seed meal and proteins on rumen fermentation in vitro. Annual Report, Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim, Stuttgart, Germany.Google Scholar
Makkar, HPS, Francis, G and Becker, K 2007. Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems. Animal 1, 1371–1391.Google Scholar
Maldini, M, Maksoud, SA, Natella, F, Montoro, P, Petretto, GL, Foddai, M, De Nicola, GR, Chessa, M and Pintore, G 2014. Moringa oleifera: study of phenolics and glucosinolates by mass spectrometry. Journal of Mass Spectrometry 49, 900–910.Google Scholar
Males, JR and Purser, DB 1970. Relationship between rumen ammonia levels and the microbial population and volatile fatty acid proportions in faunated and defaunated sheep. Applied Microbiology 19, 483–490.Google Scholar
Martin, C, Rouel, J, Jouany, JP, Doreau, M and Chilliard, Y 2008. Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed, or linseed oil. Journal of Animal Science 86, 2642–2650, https://doi.org/10.2527/jas.2007-0774.Google Scholar
McAllister, TA, Bae, HD, Jones, GA and Cheng, KJ 1994. Microbial attachment and feed digestion in the rumen. Journal of Animal Science 72, 3004–3018.Google Scholar
McDougall, EI 1948. Studies on ruminant saliva. 1. The composition and output of sheep’s saliva. Biochemical Journal 43, 99–109.Google Scholar
Melesse, A 2012. Assessing the feeding values of leaves, seeds and seeds-removed pods of Moringa stenopetala using in vitro gas production technique. African Journal of Biotechnology 11, 11342.Google Scholar
Melesse, A, Steingass, H, Boguhn, J and Rodehutscord, M 2013. In vitro fermentation characteristics and effective utilisable crude protein in leaves and green pods of Moringa stenopetala and Moringa oleifera cultivated at low and mid‐altitudes. Journal of Animal Physiology and Animal Nutrition 97, 537–546.Google Scholar
Mendieta-Araica, B, Spörndly, E, Reyes-Sánchez, N, Spörndly, R and Sveriges, L 2011. Feeding Moringa oleifera fresh or ensiled to dairy cows-effects on milk yield and milk flavor. Tropical Animal Health and Production 43, 1039–1047.Google Scholar
Moreira, MN, AMdA Bezerra, S, Carneiro, LR, Morais RKdO, H and Medeiros, FFd 2016. Effect of replacement of sugarcane by oilseed press cakes in greenhouse gases and volatile fatty acids production in vitro. Acta Scientiarum Animal Sciences 38, 293–300.Google Scholar
National Research Council 2001. Nutrient requirements of dairy cattle, 7th revised edition. National Academies Press, Washington, DC, USA.Google Scholar
Ogunsina, BS, Indira, TN, Bhatnagar, AS, Radha, C, Debnath, S and Gopala Krishna, AG 2014. Quality characteristics and stability of Moringa oleifera seed oil of Indian origin. Journal of Food Science and Technology 51, 503–510.Google Scholar
Olivares-Palma, SM, Meale, SJ, Pereira, LGR, Machado, FS, Carneiro, H, Lopes, FCF, Maurício, RM and Chaves, AV 2013. In vitro fermentation, digestion kinetics and methane production of oilseed press cakes from biodiesel production. Asian-Australas Journal of Animal Science 26, 1102–1110.Google Scholar
Palmquist, DL 1994. The role of dietary fats in efficiency of ruminants. Journal of Nutrition 124, 1377–1382.Google Scholar
Premi, M and Sharma, HK 2017. Effect of extraction conditions on the bioactive compounds from Moringa oleifera (PKM 1) seeds and their identification using LC–MS. Journal of Food Measurement and Characterization 11, 213–225.Google Scholar
Rhine, ED, Sims, GK, Mulvaney, RL and Pratt, EJ 1998. Improving the Berthelot reaction for determining ammonium in soil extracts and water. Soil Science Society of America Journal 62, 473–480.Google Scholar
Rodrigues, JPP, de Paula, RM, Rennó, LN, Fontes, MMS, Machado, AF, de Valadares, C, Filho, S, Huhtanen, P and Marcondes, MI 2017. Short-term effects of soybean oil supplementation on performance, digestion, and metabolism in dairy cows fed sugarcane-based diets. Journal of Dairy Science 10, 4435–4447.Google Scholar
Salem, HB and Makkar, HPS 2009. Defatted Moringa oleifera seed meal as a feed additive for sheep. Animal Feed Science and Technology 150, 27–33.Google Scholar
SAS Institute 2018. SAS/STAT user’s guide. SAS Institute Inc., Cary, NC, USA.Google Scholar
Soliva, CR, Kreuzer, M, Foidl, N, Foidl, G, Machmüller, A and Hess, HD 2005. Feeding value of whole and extracted Moringa oleifera leaves for ruminants and their effects on ruminal fermentation in vitro. Journal of Food Science and Technology 118, 47–62.Google Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal production. Journal of Dairy Science 74, 3583–3597.Google Scholar
Van Soest PJ 1994. Nutritional ecology of the ruminant. 2nd edition. Cornell University Press, Ithaca, NY, USA. Google Scholar
Wang, L, Chen, X and Wu, A 2016. Mini review on antimicrobial activity and bioactive compounds of Moringa oleifera. Medicinal Chemistry (Los Angeles) 6, 578–582.Google Scholar
Wang, Y, McAllister, TA, Rode, LM, Beauchemin, KA, Morgavi, DP, Nsereko, VL, Iwaasa, AD and Yang, W 2001. Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the Rumen Simulation Technique (Rusitec). British Journal of Nutrition 85, 325–332.Google Scholar
Wang, Y, McAllister, TA, Yanke, LJ, Xu, ZJ, Cheeke, PR and Cheng, KJ 2000. In vitro effects of steroidal saponins from Yucca schidigera extract on rumen microbial protein synthesis and ruminal fermentation. Journal of the Science of Food and Agriculture 80, 2114–2122.Google Scholar