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Reference genes selection for real-time quantitative PCR analysis in mouse germinal vesicle oocytes

Published online by Cambridge University Press:  23 September 2019

M.A. Filatov Open the ORCID record for M.A. Filatov [Opens in a new window] ,
D.A. Nikishin ,
Y.V. Khramova  and
M.L. Semenova
M.A. Filatov*
Affiliation:
Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory, 1, bld. 12, Moscow, 119991, Russia
D.A. Nikishin
Affiliation:
Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory, 1, bld. 12, Moscow, 119991, Russia N.K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova Street, 26, Moscow, 119334, Russia
Y.V. Khramova
Affiliation:
Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory, 1, bld. 12, Moscow, 119991, Russia
M.L. Semenova
Affiliation:
Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory, 1, bld. 12, Moscow, 119991, Russia
*
Address for correspondence: M.A. Filatov. Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory, 1, bld. 12, Moscow, 119991, Russia. Tel: +7 495 939 39 00. Fax: +7 495 939 43 09. E-mail: maxfilat@yandex.ru
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Summary

Reference gene selection in mouse oocytes is an important task required to perform further adequate analysis of target gene expression levels. In the current work we have analyzed expression stability of the seven most commonly used reference genes (Actb, Eef1e1, Gapdh, H2afz, Ppia, Rpl4 and Ubc) in mouse oocytes at the germinal vesicle (GV) stage. We have performed analysis of expression stability of the above-mentioned reference genes with the three most commonly used software tools: geNorm, BestKeeper and NormFinder. Taking into account the results obtained from all of these programmes Gapdh, Rpl4 and H2afz seem to be suitable candidate reference genes in GV oocytes of mouse.

Keywords

Germinal vesicleMiceOocytePCRReference gene
Type
Research Article
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 392 - 397
Copyright
© Cambridge University Press 2019 

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