Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-23T12:50:48.696Z Has data issue: false hasContentIssue false
  • English
  • Français

Evidence for selection against tetraploid cells in tetraploid[harr ]diploid mouse chimaeras before the late blastocyst stage

Published online by Cambridge University Press:  01 December 1998

CLARE A. EVERETT  and
JOHN D. WEST
CLARE A. EVERETT
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, United Kingdom
JOHN D. WEST
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, United Kingdom
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Tetraploid (4n) cells do not contribute equally to all tissues of midgestation mouse chimaeras and mosaics. Our previous studies of early blastocysts showed that 4n cells are preferentially allocated to the mural trophectoderm of the early blastocyst and this may contribute to the restricted distribution pattern seen at later stages. In this study of later-stage blastocysts we found evidence for selection against 4n cells. The contribution of 4n cells to 4n[harr ]2n chimaeric blastocysts decreased between E3·5 and E4·5 days, whereas the composition of 2n[harr ]2n controls changed little over this period. These results suggest that, prior to implantation, blastocysts have already lost some tetraploid cells from their embryonic and extra-embryonic lineages due to a combination of preferential allocation of 4n cells to the mural trophectoderm and selection against 4n cells throughout the embryo.

Type
Research Article
Information
Genetics Research , Volume 72 , Issue 3 , December 1998 , pp. 225 - 228
Copyright
© 1998 Cambridge University Press