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Growth-promoting effects of caseinomacropeptide from cow and goat milk on probiotics

Published online by Cambridge University Press:  27 November 2012

Gilles Robitaille
Gilles Robitaille*
Affiliation:
Food Research and Development Centre (FRDC), Agriculture and Agri-Food Canada, St Hyacinthe, Quebec, J2S 8E3, Canada
*
For correspondence; e-mail: gilles.robitaille@agr.gc.ca
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Abstract

Caseinomacropeptide (CMP), a 7-kDa phosphoglycopolypeptide fragment released from κ-casein during milk renneting, is heterogeneous with respect to post-translational glycosylation. Several studies have reported that CMP has growth-promoting activity on lactic acid bacteria belonging to the genera Bifidobacterium. The aim of this study was to evaluate the effect of glycosylation and sequence variations between bovine and caprine CMP on the growth of two probiotics: Lactobacillus rhamnosus RW-9595-M and Bifidobacterium thermophilum RBL67. The growth-promoting activities of CMP (mixture of glycosylated (gCMP) and non-glycosylated (aCMP) fractions), aCMP and gCMP were measured in a basal minimal culture medium using turbidimetric microplate assay at 37 °C. Supplementation of the culture media at 2 mg/ml significantly improved maximum growth by 1·5 to 1·8 times depending on the strain, the additive (CMP, aCMP, gCMP), and the bovine or caprine origin (P < 0·05). CMP preparations also decreased the time needed to reach the inflexion point of the growth curve and increase the cell density at that time (P < 0·05). The effects of CMP preparations were dose dependent and significantly superior to the effect of bovine β-lactoglobulin added to the culture media. As gCMP and aCMP were as efficient as bovine and caprine CMP (P > 0·1), it was concluded that the presence of oligosaccharides linked to CMP was not essential for growth-promoting activity of CMP.

Keywords

Glycomacropeptideκ-caseingrowth factorBifidobacteriumLactobacillus
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 1 , February 2013 , pp. 58 - 63
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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References

Araya, M, Morelli, L, Reid, G, Sanders, ME, Stanton, C, Pineiro, M & Ben Embarek, P 2002 Guidelines for the evaluation of probiotics in food. Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food, London (ON, Canada). ftp://ftp.fao.org/es/esn/food/wgreport2.pdfGoogle Scholar
Azuma, N, Yamauchi, K & Mitsuoka, T 1984 Bifidus growth-promoting activity of a glycomacropeptide derived from human κ-casein. Agricultural and Biological Chemistry 48 2159–2162Google Scholar
Brück, WM, Graverholt, G & Gibson, GR 2002 Use of batch culture and a two-stage continuous culture system to study the effect of supplemental α-lactalbumin and glycomacropeptide on mixed populations of human gut bacteria. FEMS Microbiology Ecology 41 231–237Google Scholar
Brück, WM, Redgrave, M, Tuohy, KM, Lönnerdal, B, Graverholt, G, Hernell, O & Gibson, GR 2006 Effects of bovine α-lactalbumin and casein glycomacropeptide-enriched infant formulae on faecal microbiota in healthy term infants. Journal of Pediatric Gastroenterology and Nutrition 43 673–679Google Scholar
CFIA (Canadian Food Inspection Agency) 2009 Probiotic claims.Chapter 8, Section 8.7. http://www.inspection.gc.ca/english/fssa/labeti/guide/ch8ae.shtmlGoogle Scholar
Chabot, S, Yu, HL, De, Léséleuc, L, Cloutier, D, Van Calsteren, MR, Lessard, M, Roy, D, Lacroix, M & Oth, D 2001 Exopolysaccharides from Lactobacillus rhamnosus RW-9595M stimulate TNF, IL-6 and IL-12 in human and mouse cultured immunocompetent cells, and IFN-γ in mouse splenocytes. Lait 81 683–697Google Scholar
Champagne, CP & Fustier, P 2007 Microencapsulation for the improved delivery of bioactive compounds into foods. Current Opinion in Biotechnology 18 184–190CrossRefGoogle ScholarPubMed
Champagne, CP, Gardner, NJ & Roy, D 2005 Challenges in the addition of probiotic cultures to foods. Critical Reviews in Food Science and Nutrition 45 61–84Google Scholar
Cicvárek, J, Čurda, L, Elich, O, Dvořáková, E & Dvořák, M 2010 Effect of caseinomacropeptide concentrate addition on the growth of bifidobacteria. Czech Journal of Food Sciences 28 485–494Google Scholar
Coolbear, KP, Elgar, DF & Ayers, JS 1996 Profiling of genetic variants of bovine κ-casein macropeptide by electrophoretic and chromatographic techniques. International Dairy Journal 6 1055–1068CrossRefGoogle Scholar
Dalgaard, P 1994 Estimation of bacterial growth rates from turbidimetric and viable count data. International Journal of Food Microbiology 23 391–404CrossRefGoogle ScholarPubMed
Dave, RI & Shah, NP 1998 Ingredient supplementation effects on viability of probiotic bacteria in Yogurt. Journal of Dairy Science 81 2804–2816Google Scholar
Farrell, HM Jr, Jimenez-Flores, R, Bleck, GT, Brown, EM, Butler, JE, Creamer, LK, Hicks, CL, Hollar, CM, Ng-Kwai-Hang, KF & Swaisgood, HE 2004 Nomenclature of the proteins of cows' milk–Sixth revision. Journal of Dairy Science 87 1641–1674Google Scholar
Fukuda, SP, Roig, SM & Prata, LF 2004 Correlation between acidic ninhydrin and HPLC methods to evaluate fraudulent addition of whey in milk. Lait 84 501–512Google Scholar
Gomes, AMP, Malcata, FX & Klaver, FAM 1998 Growth enhancement of Bifidobacterium lactis Bo and Lactobacillus acidophilus Ki by milk Hydrolyzates. Journal of Dairy Science 81 2817–2825Google Scholar
Idota, T, Kawakami, H & Nakajima, I 1994 Growth-promoting effects of N-Acetyl- neuraminic acid-containing substances on Bifidobacteria. Bioscience, Biotechnology, and Biochemistry 58 1720–1722Google Scholar
Ibrahim, SA & Bezkorovainy, A 1994 Growth-promoting factors for Bifidobacterium longum. Journal of Food Science 59 189–191CrossRefGoogle Scholar
Janer, C, Pelaez, C & Requena, T 2004 Caseinomacropeptide and whey protein concentrate enhance Bifidobacterium lactis growth in milk. Food Chemistry 86 263–267CrossRefGoogle Scholar
Klaver, FAM, Kingma, F & Weerkamp, AH 1993 Growth and survival of bifidobacteria in milk. Netherland milk Dairy Journal 47 151–164Google Scholar
Mollé, D & Leonil, J 2005 Quantitative determination of bovine κ-casein macropeptide in dairy products by Liquid chromatography/Electrospray coupled to mass spectrometry (LC-ESI/MS) and Liquid chromatography/Electrospray coupled to tamdem mass spectrometry (LC-ESI/MS/MS). International Dairy Journal 15 419–428Google Scholar
Moreno, FJ, Olano, A & Villamiel, M 2001 N-acetyl-and N-glycolyl-neuraminic acid content in κ-casein macropeptides. Milchwissenschaft 56 669–671Google Scholar
Morishita, T, Deguchi, Y & Yajima, M 1981 Multiple nutritional requirements of lactobacilli: genetic lesions affecting amino acid biosynthetic pathways. Journal of Bacteriology 148 64–71Google Scholar
Petschow, BW & Talbott, RD 1991 Response of Bifidobacterium species to growth promoters in human and cow milk. Pediatric Research 29 208–213Google Scholar
Poch, M & Bezkorovainy, A 1988 Growth-enhancing supplements for various species of the genus Bifidobacterium. Journal of Dairy Science 71 3214–3221Google Scholar
Poch, M & Bezkorovainy, A 1991 Bovine milk κ-casein trypsin digest is a growth enhancer for the genus Bifidobacterium. Journal of Agricultural and Food Chemistry 39 73–77Google Scholar
Proulx, M, Ward, P, Gauthier, SF & Roy, D 1994 Comparison of bifidobacterial growth-promoting activity of ultrafiltered casein hydrolyzate fractions. Lait 74 139–152Google Scholar
Robitaille, G, Lapointe, C, Leclerc, D & Britten, M 2012 Effect of pepsin-treated bovine and goat caseinomacropeptide on Escherichia coli and Lactobacillus rhamnosus in acidic conditions. Journal of Dairy Science 95 1–8Google Scholar
Saxelin, M, Grenov, B, Svensson, U, Fondén, R, Reniero, R & Mattila-Sandholm, T 1999 The technology of probiotics. Trends in Food Science and Technology 10 387–392Google Scholar
Shah, NP 2007 Functional cultures and health benefits. International Dairy Journal 17 1262–1277Google Scholar
Thomä-Worringer, C, Sorensen, J & Lopez-Fandino, R 2006 Health effects and technological features of caseinomacropeptide: technological and health aspects of bioactive components of milk. International Dairy Journal 16 1324–1333Google Scholar
Touré, R, Kheadr, E, Lacroix, C, Moroni, O & Fliss, I 2003 Production of antibacterial substances by bifidobacterial isolates from infant stool active against Listeria monocytogenes. Journal of Applied Microbiology 95 1058–1069Google Scholar
Von Ah, U, Mozzetti, V, Lacroix, C, Kheadr, EE, Fliss, I & Meile, L 2007 Classification of a moderately oxygen-tolerant isolate from baby faeces as Bifidobacterium thermophilum. BMC Microbiology 79Google Scholar
Yonezawa, S, Xiao, JZ, Odamaki, T, Ishida, T, Miyaji, K, Yamada, A, Yaeshima, T & Iwatsuki, K 2010 Improved growth of Bifidobacteria by cocultivation with Lactococcus lactis subspecies lactis. Journal of Dairy Science 93 1815–1823Google Scholar