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Levels of infection with the lungworm Angiostrongylus cantonensis in terrestrial snails from Thailand, with Cryptozona siamensis as a new intermediate host

Published online by Cambridge University Press:  15 January 2016

A. Vitta*
Affiliation:
1Department of Microbiology and Parasitology, Faculty of Medical Science 2Centre of Excellence in Medical Biotechnology (CEMB) 3Center of Excellence for Biodiversity, Naresuan University, Phitsanulok 65000, Thailand
W. Polsut
Affiliation:
1Department of Microbiology and Parasitology, Faculty of Medical Science
C. Fukruksa
Affiliation:
1Department of Microbiology and Parasitology, Faculty of Medical Science
T. Yimthin
Affiliation:
1Department of Microbiology and Parasitology, Faculty of Medical Science
A. Thanwisai
Affiliation:
1Department of Microbiology and Parasitology, Faculty of Medical Science 2Centre of Excellence in Medical Biotechnology (CEMB) 3Center of Excellence for Biodiversity, Naresuan University, Phitsanulok 65000, Thailand
P. Dekumyoy
Affiliation:
4Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
*

Abstract

Angiostrongylus cantonensis is primarily considered an emerging infectious agent of eosinophilic meningitis or meningoencephalitis with a worldwide distribution. Rodents and snails are important invasive hosts for transmission and expansion of A. cantonensis. The objective of this study was to investigate infection levels of A. cantonensis in snails, the most important natural intermediate host. Our study location was Mueang Kamphaeng Phet district, Kamphaeng Phet Province, and was undertaken between October and December 2012. A total of 2228 freshwater and terrestrial snails were collected, comprising 1119 Filopaludina spp., 409 Pomacea caniculata, 275 Achatina fulica and 425 Cryptozona siamensis. Angiostrongylus larvae were isolated by artificial digestion methods following Baermann's techniques. A low prevalence and intensity of A. cantonensis were observed in A. fulica, while higher numbers were found in C. siamensis. None of the Filopaludina spp. and Pomacea caniculata were infected with A. cantonensis. Molecular characterization was performed by analysing the 264 bp of cytochrome c oxidase subunit I (COI). Three COI sequences of Angiostrongylus were identical to A. cantonensis with 91–99% identity. Cryptozona siamensis has not previously been recorded as an intermediate host for A. cantonensis in Thailand. The infection of A. cantonensis identified in the natural intermediate hosts is new and important information to assist in the prevention and control of human angiostrongyliasis.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2016 

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References

Crook, J.R., Fulton, S.E. & Supanwong, K. (1968) Ecological studies on the intermediate and definitive hosts of Angiostrongylus cantonensis (Chen, 1935) in Thailand. Annals of Tropical Medicine and Parasitology 62, 2744.Google Scholar
Cross, J.H. & Chen, E.R. (2007) Angiostrongyliasis. pp. 263290 in Murrell, K.D. & Fried, B. (Eds) Food-borne parasitic zoonoses: Fish and plant-borne parasites. New York, Springer Science and Business Media LLC.Google Scholar
Eamsobhana, P. (2006) The rat lungworm Parastrongylus (Angiostrongylus ) cantonensis: parasitology, immunology, eosinophilic meningitis, epidemiology and laboratory diagnosis. 156 pp. Bangkok, Wankaew (IQ) Book Center.Google Scholar
Eamsobhana, P. (2013) Angiostrongyliasis in Thailand: epidemiology and laboratory investigations. Hawaii Journal of Medicine and Public Health 72, 2832.Google ScholarPubMed
Espírito-Santo, M.C., Pinto, P.L., Mota, D.J. & Gryschek, R.C. (2013) The first case of Angiostrongylus cantonensis eosinophilic meningitis diagnosed in the city of São Paulo, Brazil. Revista do Instituto de Medicina Tropical de São Paulo 55, 129132.CrossRefGoogle ScholarPubMed
Graeff-Teixeira, C. (2007) Expansion of Achatina fulica in Brazil and potential increased risk for angiostrongyliasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 101, 743744.CrossRefGoogle ScholarPubMed
Harinasuta, C., Setasuban, P. & Radomyos, P. (1965) Observations on Angiostrongylus cantonensis in rats and mollusks in Thailand. Journal of the Medical Association of Thailand 48, 158172.Google Scholar
Haswell-Elkins, M.R., Elkins, D.B., Sithithaworn, P., Treesarawat, P. & Kaewkes, S. (1991) Distribution patterns of Opisthorchis viverrini within a human community. Parasitology 103, 97101.Google Scholar
Jefferies, R., Shaw, S.E., Viney, M.E. & Morgan, E.R. (2009) Angiostrongylus vasorum from South America and Europe represent distinct lineages. Parasitology 136, 107115.Google Scholar
Kim, J.R., Hayes, K.A., Yeung, W.N. & Cowie, R.H. (2014) Diverse gastropod hosts of Angiostrongylus cantonensis, the rat lungworm, globally and with a focus on the Hawaiian Islands. Plos One 9, e94969.CrossRefGoogle ScholarPubMed
Ko, R.C. (1991) Current status of food-borne parasitic zoonoses in Hong Kong. The Southeast Asian Journal of Tropical Medicine and Public Health 22, 4247.Google Scholar
Maldonado, A. Jr, Simões, R.O., Oliveira, A.P., Motta, E.M., Fernandez, M.A., Pereira, Z.M., Monteiro, S.S., Torres, E.J. & Thiengo, S.C. (2010) First report of Angiostrongylus cantonensis (Nematoda: Metastrongylidae) in Achatina fulica (Mollusca: Gastropoda) from Southeast and South Brazil. Memórias do Instituto Oswaldo Cruz 105, 938941.Google Scholar
Namue, C. & Wongsawad, C. (1997) A survey of helminth infection in rats (Rattus spp.) from Chiang Mai moat. The Southeast Asian Journal of Tropical Medicine and Public Health 28, 179183.Google Scholar
Pal, P. & Lewis, J.W. (2004) Parasite aggregations in host populations using a re-formulated negative binomial distribution. Journal of Helminthology 78, 5761.CrossRefGoogle Scholar
Pipitgool, V., Sithithaworn, P., Pongmuttasaya, P. & Hinz, E. (1997) Angiostrongylus infections in rats and snails in northeast Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health 28, 190193.Google Scholar
Punyagupta, S. (1965) Eosinophilic meningoencephalitis in Thailand: summary of nine cases and observations on Angiostrongylus cantonensis as a causative agent and Pila ampullacea as a new intermediate host. The American Journal of Tropical Medicine and Hygiene 14, 370374.Google Scholar
Radomyos, P., Tungtrongchitr, A., Praewanich, R., Khewwatchan, P., Kantangkul, T., Junlananto, P. & Ayudhya, S.I. (1994) Occurrence of the infective stage of Angiostrongylus cantonensis in the yellow tree monitor (Varanus bengalensis) in five provinces of Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health 25, 498500.Google Scholar
Setasuban, P., Vairasthira, S. & Harinasuta, C. (1968) The preliminary observation on natural infection of rat lung worm (Angiostrongylus cantonensis) in rodents and intermediate host in Thailand. Journal of the Medical Association of Thailand 51, 156157.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 27312739.CrossRefGoogle ScholarPubMed
Teem, J.L., Qvarnstrom, Y., Bishop, H.S., da Silva, A.J., Carter, J., White-McLean, J. & Smith, T. (2013) The occurrence of the rat lungworm, Angiostrongylus cantonensis, in nonindigenous snails in the Gulf of Mexico region of the United States. Hawaii Journal of Medicine and Public Health 72, 1114.Google Scholar
Tesana, S., Srisawangwong, T., Sithithaworn, P., Laha, T. & Andrews, R. (2009) Prevalence and intensity of infection with third stage larvae of Angiostrongylus cantonensis in mollusks from Northeast Thailand. The American Journal of Tropical Medicine and Hygiene 80, 983987.Google Scholar
Thiengo, S.C., Maldonado, A., Mota, E.M., Torres, E.J., Caldeira, R., Carvalho, O.S., Oliveira, A.P., Simões, R.O., Fernandez, M.A. & Lanfredi, R.M. (2010) The giant African snail Achatina fulica as natural intermediate host of Angiostrongylus cantonensis in Pernambuco, northeast Brazil. Acta Tropica 115, 194199.Google Scholar
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.Google Scholar
Vitta, A. (2012) Diagnosis of human angiostrongyliasis. Asian Biomedicine 6, 141150.Google Scholar
Vitta, A., Polseela, R., Nateeworanart, S. & Tattiyapong, M. (2011) Survey of Angiostrongylus cantonensis in rats and giant African land snails in Phitsanulok province, Thailand. Asian Pacific Journal Tropical Medicine 4, 597599.CrossRefGoogle ScholarPubMed
Wang, Q.P., Lai, D.H., Zhu, X.Q., Chen, X.G. & Lun, Z.R. (2008) Human angiostrongyliasis. Lancet Infectious Diseases 8, 621630.Google Scholar