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Isoflavones content in soybean and soybean milk in Rwanda

Published online by Cambridge University Press:  03 February 2017

M. Niyibituronsa ,
A.N. Onyango ,
S. Gaidashova ,
S. Imathiu ,
M. Uwizerwa ,
I. Wanjuki ,
F. Munga ,
E. Ochieng ,
J. Birungi  and
S. Ghimire
...Show all authors
M. Niyibituronsa
Affiliation:
Rwanda Agriculture Board, Rwanda Jomo Kenyatta University of Agriculture and Technology, Kenya Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya
A.N. Onyango
Affiliation:
Jomo Kenyatta University of Agriculture and Technology, Kenya
S. Gaidashova
Affiliation:
Rwanda Agriculture Board, Rwanda
S. Imathiu
Affiliation:
Jomo Kenyatta University of Agriculture and Technology, Kenya
M. Uwizerwa
Affiliation:
Rwanda Agriculture Board, Rwanda
I. Wanjuki
Affiliation:
Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya
F. Munga
Affiliation:
Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya
E. Ochieng
Affiliation:
Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya
J. Birungi
Affiliation:
Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya
S. Ghimire
Affiliation:
Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya
S. Mutiga
Affiliation:
Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya Kansas State University, USA
J. Harvey
Affiliation:
Biosciences eastern and central Africa-International Livestock Research Institute Hub, Kenya Kansas State University, USA
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Abstract

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Information
Proceedings of the Nutrition Society , Volume 76 , Issue OCE1: Winter Meeting, 6–7 December 2016, Diet, Nutrition and Mental Health and Wellbeing , 2017 , E23
Copyright
Copyright © The Authors 2017 

The most important attribute of soybean is their health benefits due to protein and isoflavones that prevent and treat many chronic diseases(Reference Jooyandeh1). Isoflavones found in soybean have anti-carcinogenetic activities, prevent cardio-vascular diseases, prevent osteoporosis, have antioxidant activities and alleviate menopausal symptoms(Reference Alekel, St Germain and Peterson2). The countries with highest consumption of phyto-oestrogen (isoflavone and lignan) have low rate of breast cancer(Reference Anderson and Michelle Cotterchio3). However there is limited information on isoflavones content in soybean produced in Rwanda. The study aimed to determine isoflavones content in soybean varieties grown in Rwanda and soybean milk processed using water extraction method(Reference Hosken4).

Isoflavones were determined in five varieties grown in South and Eastern zones using AOAC Official Method 2008·03(5). Triplicates samples of soybean flour (N = 10) and soybean milk (N = 10) were used for study. The extracts analysed on a reverse-phase (C-18) UPLC with UV detection (λnm = 260). The target analytes were aglycon isoflavones: Genistein and Daidzein, and their glucoside forms: Daidzin and Genistin.

The total isoflavones varied between 1866·2 and 4627·0μg/g in soybean flour and between 140·1 and 277·5μg/g for soybean milk. Squire variety had the highest isoflavone content with twice more than the other test varieties in both flour and milk. These findings will inform Rwandese breeders on how best to adjust their programs in order to improve the nutritional content of cultivated soybean and the Rwandan wellbeing.

This project was supported by the BecA-ILRI Hub through the Africa Biosciences Challenge Fund (ABCF) program.

References

1. Jooyandeh, H (2011) MEJSR 7(1) 71–80.Google Scholar
2. Alekel, D L, St Germain, A, Peterson, C T et al. (2000) Am J Clin Nutr 72, 844–852.Google Scholar
3. Anderson, L N, Michelle Cotterchio, M et al. (2013) IJC 132(7), 1683–1692.Google Scholar
4. Hosken, B (1999) reviewed by Lui, KeShun, Aspen Pub Inc. Maryland.Google Scholar
5. AOAC (2008) J. AOAC International, 91(3): m489–500.Google Scholar