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Ocular pathological changes in hamsters experimentally infected with Schistosoma mansoni

Published online by Cambridge University Press:  17 February 2016

H.I.H. Ismail ,
D.S. Ashour ,
D.M. Abou Rayia  and
A.L. Ali
H.I.H. Ismail
Affiliation:
Departments of 1Medical Parasitology and
D.S. Ashour*
Affiliation:
Departments of 1Medical Parasitology and
D.M. Abou Rayia
Affiliation:
Departments of 1Medical Parasitology and
A.L. Ali
Affiliation:
Ophthalmology, Faculty of Medicine, Tanta University, Tanta, Egypt
*
*E-mail: ashourdalia@yahoo.com
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Abstract

Ocular lesions have been reported in patients with schistosomiasis; however, the problem with studying schistosomal infection of the human eye is that biopsies are almost impossible to take, and histopathological examination of suspicious lesions can only be undertaken post-mortem or after enucleation. This work aimed to study the possible effects and pathogenesis of schistosomiasis on the eye. This study involved 55 hamsters; five hamsters remained non-infected and the remaining 50 hamsters were infected with Schistosoma mansoni cercariae. Infected hamsters were sacrificed on weeks 8, 12, 16 and 20 post-infection (pi). Eye sections were prepared and stained for histopathological and immunohistochemical studies. Histopathological changes detected in hamsters infected after 16 and 20 weeks included looseness and oedema of the innermost retinal layers together with hyperplastic polypoid growth. Neither eggs nor granulomata were detected in eye sections throughout the experimental period. Deposition of S. mansoni antigen was revealed in 35% of infected hamsters. Later, on weeks 16 and 20 pi, moderate subepithelial conjuctival deposits and marked subchoroidal and scleral deposition were detected. In conclusion, the deposition of schistosomal antigen and immune complexes may play a pivotal role in the ocular changes that occur in schistosomiasis, even in the absence of detectable Schistosoma eggs. Schistosomiasis should be suspected in cases with unexplained ophthalmological findings, especially in endemic areas.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 6 , November 2016 , pp. 773 - 778
Copyright
Copyright © Cambridge University Press 2016 

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References

Bell, R.D. (1995) Schistosomiasis. Lecture notes on tropical medicine. 4th edn. pp. 221–239. Oxford, Blackwell Science.Google Scholar
Bialasiewicz, A.A., Hassenstein, A. & Schaudig, U. (2001) Subretinal granuloma, retinal vasculitis and keratouveitis with secondary subretinal glaucoma by schistosomiasis. Ophthalmologie 98, 972–975.Google Scholar
Cheever, A.W. (1993) Schistosomiasis: infection versus disease and hypersensitivity versus immunity. American Journal of Pathology 142, 699–702.Google ScholarPubMed
Corsi, A., Nucci, C., Knafelz, D., Bulgarini, D., Di Iorio, L., Polito, A., De Risi, F., Ardenti Morini, F. & Paone, F.M. (1998) Ocular changes associated with Giardia lamblia infection in children. British Journal of Ophthalmology 82, 59–62.Google Scholar
Creed, T.D. (1993) Unilateral optic atrophy presumed secondary to schistosomiasis of the optic nerve. Journal of American Optometric Association 64, 440–445.Google Scholar
Deedler, A.M., De Jonge, N., Boerman, O.C., Fillie, Y.E., Hilberath, G.W., Rotmans, J.P., Gerritse, M.J. & Lexschut, D.W. (1989) Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme linked immunosorbent assay using monoclonal antibodies. American Society of Tropical Medicine and Hygiene 40, 268–272.Google Scholar
Delgado de Souza, A.C., Teixeira Brandt, C., Ventura, L. & Orefice, F. (2002) Retinal fluorescein contrast arrival time of young patients with the hepatosplenic form of schistosomiasis mansoni. Memórias do Instituto Oswaldo Cruz 97, 161–164.Google Scholar
Dickinson, A.J., Rosenthal, A.R. & Nicholson, K.G. (1990) Inflammation of the retinal pigment epithelium: a unique presentation of ocular schistosomiasis. British Journal of Ophthalmology 74, 440–442.Google Scholar
Drury, R. & Wallington, E. (1980) Carlton's histological techniques. 5th edn. 141 pp. Oxford, Oxford University Press.Google Scholar
Eid, M.M. (1988) Studies on the immune complexes in the experimentally infected mice with Schistosoma mansoni. MD thesis in parasitology, Faculty of Medicine, Tanta University.Google Scholar
El-Dosoky, K.I., Van Marck, E.A. & Deelder, A.M. (1984) Presence of Schistosoma mansoni antigens in liver, spleen and kidney of infected mice: a sequential study. Zeitschrift für Parasitenkunde 70, 491–497.Google Scholar
El Garem, A.A. (1998) Schistosomiasis. Digestion 59, 589–605.CrossRefGoogle ScholarPubMed
El-Nouby, K.A. (2003) Cardiac involvement in experimental schistosomiasis mansoni: a study of the possible mechanism. Tanta Medical Journal 31, 1136–1141.Google Scholar
Frandsen, F. & Christensen, N.Q. (1984) An introductory guide to the identification of cercariae from African freshwater snails with special reference to cercariae of trematode species of medical and veterinary importance. Acta Tropica 41, 181–202.Google Scholar
Grobusch, M.P., Muhlberger, N., Jelinek, T., Bisoffi, Z., Corachan, M., Harms, G., Matteelli, A., Fry, G., Hatz, C., Gjorup, I., Schmid, M.L., Knobloch, J., Puente, S., Bronner, U., Kapaun, A., Clerinx, J., Nielsen, L.N., Fleischer, K., Beran, J., da Cunha, S., Schulze, M., Myrvang, B. & Hellgren, U. (2003) Imported schistosomiasis in Europe: sentinel surveillance data from TropNetEurop. Journal of Travel Medicine 10, 164–169.Google Scholar
Hsu, S.Y.L., Hsu, H.F., Mitros, F.A., Helms, C.M. & Solomon, R.I. (1980) Eosinophils as effector cells in the destruction of Schistosoma mansoni eggs in granulomas. Annals of Tropical Medicine and Parasitology 74, 179–183.Google Scholar
Jacobs, W., VanDam, G., Bogers, J., Deelder, A. & Van Marck, E. (1999) Schistosomal granuloma modulation. I. Schistosoma mansoni worm antigens CAA and CCA prime egg-antigen-induced hepatic granuloma formation. Parasitology Research 85, 7–13.Google Scholar
Kabo, A.M. & Warter, A. (1993) A propos of 1 case of ophthalmologic manifestations of Biharziasis. Bulletin de la Société de Pathologie Exotique 86, 174–175.Google Scholar
Khalil, H.M., Makled, M.K.H., El-Sebai, M.M. & El-Naggar, M.H. (1987) Detection of immune complexes in ascitic fluid of hepatosplenic schistosomiasis. Journal of the Egyptian Society of Parasitology 17, 699–705.Google Scholar
Klotz, S.A., Penn, C.C., Negvesky, G.J. & Butrus, S.I. (2000) Fungal and parasitic infections of the eye. Clinical Microbiology Reviews 13, 662–685.Google Scholar
Milligan, D. & Burns, D.A. (1988) Ectopic cutaneous schistosomiasis and schistosomal ocular inflammatory disease. British Journal of Ophthalmology 119, 793–798.Google ScholarPubMed
Newton, J.C., Kanchanaranya, C. & Previte, L.R. (1968) Intraocular Schistosoma mansoni . American Journal of Ophthalmology 65, 774–778.Google Scholar
Orefice, F., Simal, C.J. & Pitella, J.E. (1985) Schistosomotic choroiditis. I. Funduscopic changes and differential diagnosis. British Journal of Ophthalmology 69, 294–299.Google Scholar
Peters, P.A. & Warren, K.S. (1969) A rapid method for infecting mice and other laboratory animals with Schistosoma mansoni by subcutaneous injection. Journal of Parasitology 55, 558–561.Google Scholar
Pittella, J.E. & Orefice, F. (1985) Schistosomotic choroiditis. II. Report of first case. British Journal of Ophthalmology 69, 300–302.Google Scholar
Randriamora, J.T.M., Rabarijaona, H.Z., Rabearivony, N., Bernardin, P. & Rasoavelonoro, V.A. (2004) Bulbar subconjunctival tumor secondary to periocular schistosome granuloma. Journal Français d'Ophtalmologie 27, 1043–1045.CrossRefGoogle ScholarPubMed
Remígio, M.C., Brandt, C.T., Pontes Filho, N.T. & Albuquerque, M.C. (2009) Histological and histomorphometric evaluation of the retina of mice infected with Schistosoma mansoni in its hepatosplenic form. Acta Cirurgica Brasileira 24, 502–506.Google Scholar
Rogers, D.G. (1985) Application of the peroxidase anti-peroxidase technique for the detection of enterotoxigenic Escherichia coli K99 pilus antigen in paraffin-embeded tissues. Journal of Histochemistry and Cytochemistry 33, 762–766.Google Scholar
Snell, R.S. & Lemp, M.A. (1998) Clinical anatomy of the eye. 2nd edn. Malden, Massachusetts, Blackwell Science.Google Scholar
Sobh, M.A. (1994) Schistosomal glomerulonephritis, an update. Postgraduate Doctor 17, 28–34.Google Scholar
Stein, P.C. & Char, D.H. (1982) Intraocular granuloma: a Schistosoma mansoni model of ocular inflammation. Investigative Ophthalmology & Visual Science 23, 479–488.Google Scholar
UL'danov, G.A. (1978) Schistosomal maculitis. Oftalmologicheskii Zhurnal 33, 380–381.Google Scholar
Vedy, J., Carrica, A., Rivaud, C., Chanut, G. & Graveline, J. (1979) A case of retinitis in a patient with schistosomiasis. Médecine Tropicale 39, 603–609.Google Scholar
WHO (2014) Schistosomiasis. Fact Sheet 115. Geneva, World Health Organization. Available at http://www.who.int/mediacentre/factsheets/fs115/en/ (accessed accessed 15 May 2015).Google Scholar
Wilson, M.S., Mentink-Kane, M.M., Pesce, J.T., Ramalingam, T.R., Thompson, R. & Wynn, T.A. (2007) Immunopathology of schistosomiasis. Immunology and Cell Biology 85, 148–154.Google Scholar
Yassien, N.A. (2001) Immunohistochemical study of schistosomal soluble egg antigen in male genital organs of Schistosoma mansoni infected mice. Journal of the Egyptian Society of Parasitology 31, 381–388.Google Scholar
Yorston, D.H. & Murray McGavin, D.D. (2009) Ophthalmology in the tropics and subtropics. pp. 283–332 in Cook, G.C. & Zumla, A.I. (Eds) Manson's tropical diseases. 22nd edn. London. Saunders Elsevier.Google Scholar