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Vitrification of rat blastocysts developed in vitro

Published online by Cambridge University Press:  26 September 2008

R Nakamichi ,
S Ohboshi  and
N Fujihara
R Nakamichi
Affiliation:
Department of Animal Sciences, College of Agriculture, Kyushu University, Fukuoka, Japan.
S Ohboshi
Affiliation:
Department of Animal Sciences, College of Agriculture, Kyushu University, Fukuoka, Japan.
N Fujihara*
Affiliation:
Department of Animal Sciences, College of Agriculture, Kyushu University, Fukuoka, Japan.
*
Noboru Fujihara, Phd, Department of Animal Sciences, College of Agriculture, Kyushu University 46-06, Hakozaki, Fukuoka 812, Japan.
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Summary

Approximately 50% of rat 8-cell embryos obtained from the oviduct on day 4 of pregnancy developed to the balstocyst stage after 18 h of incubation in vitro. The embroys were compared with in vivo blastocysts derived from the uterus on day 5 of pregnancy as regards their response to vitrification treatment. Before vitrification, both types of embryos were exposed to vitrification solution, and subsequent embryonic development was inhibited with increasing time of exposure. A greater suppression of development after exposure was observed in the embryos cultured in vitro compared with in vivo blastocysts. However, development was gradually restored as in vitro incubation was continued. The response of embryos to vitrification and warming treatments was shown to be almost the same for both in vitro and in vivo blastocysts, though the former were significantly (p < 0.05) less tolerant of exposure to vitrification solution. These results suggest that in vitro blastocysts were much more susceptible to vitrification solution than in vivo blastocysts. The degree of damage caused by vitrification and thawing treatments, and the inhibition of in vitro embryonic development, were significantly (p < 0.05) more serious for in vitro blastocysts.

Keywords

BlastocystIn vitro cultureRatvitrification
Type
Article
Information
Zygote , Volume 1 , Issue 4 , November 1993 , pp. 281 - 285
Copyright
Copyright © Cambridge University Press 1993

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References

Anderson, G.B. & Foote, R.H. (1975 a). Development of rabbit embryos in vitro and in vivo following stronge of the two-cell stage at 10°C. J.Reprod. Fert. 45, 151–3.Google Scholar
Anderson, G.B. & Foote, R.H. (1975 b). Effects of low temperature upon subsequent nucleic acid and protein synthesis of rabbit embroyos. Exp. Cell Res. 90, 73–8.Google Scholar
Bowman, p. & McLaren, A. (1970). Cleavage rate of mouse embryos in vivo and in vitro. J. Embroyol. Exp. Morphol. 24, 203–7.Google Scholar
Edirisinghe, W.R., Wales, R.G. & Pike, I.L. (1984). Degradation of biochemical pools labelled with [14C] glucose during culture of 8-cell and morula-early blastocyst-stage mouse embryos in vitro and in vivo. J. Reprod. Fert. 72, 59–65.Google Scholar
Fischer, B. (1987). Developmental retardation in cultured preimplantation rabbit embryos. J. Reprod. Fert. 79, 115–23.Google Scholar
Iwasakai, S., Yoshida, N., Ushijima, H., Watanabe, S., & Nakahara, T. (1990). Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo. J. Report. Fert. 90, 279–84.Google Scholar
Jung, T. (1989). Protein synthesis and degradation in noncultured and in vitro cultured rabbit blastocysts. J. Reprod. Fert. 86, 507–12.Google Scholar
Kane, M.T. (1983). Variability in different lots of commercial bovine serum albumin affects cell multiplication and hatching of rabbit blastocysts in culture. J. Repord. Fert. 69, 555–8.Google Scholar
Kane, M.T. (1985). A low molecular weight extract of bovine serurn albumin stimulates rabbit blastocyst cell division and expansion in vitro. J. Reprod. Fert. 73, 147–50.Google Scholar
Ohboshi, S., Nakamichi, R. & Fujihara, N. 1993. Cryopreservation of rat blastocysts by vitrification using ethylene glycol, polyethylene glycol and sucrose. J. Mamm. Ova Res. 10 in press.Google Scholar
Papaioannou, V.E., & Ebert, K.M. (1986). Development of fertilized embryos transferred to oviducts of immature mice. J. Reprod. Fert. 76, 603–8.Google Scholar
Sellens, M.H., Stein, S., & Sherman, M.T. 1981. Protein and free amino acid content in preimplantation mouse embryos and in blastocysts under various culture conditions. J. Reprod. Fert. 61, 307–15.CrossRefGoogle Scholar
Steel, R.G.D., & Torrie, J.H. 1980. Principles and Procedures of Statistics. 2nd edn, pp. 469–520. New York: McGraw-Hill.Google Scholar
Streffer, C.van Beuningen, D., Molls, M., Zamboglou, N., & Schulz, S. (1980). Kinetics of cell proliferation in the preimplanted mouse embryo in vivo and in vitro. Cell Tissue Kinet. 13, 135–43.Google Scholar
Smorag, Z. & Gajda, B. 1991. Vitrification of non-cultured and cultured rabbit embryos. Anim.Reprod.Sci. 26, 151–8.Google Scholar
Techakumphu, M., Winterberger-Torres, S., Sevellec, C., & Menezo, Y. 1987. Survival of rabbit embryos after culture or culture/freezing. Anim. Reprod. Sci. 13, 221–9.Google Scholar