Skip to main content Accessibility help
×
Home

Treatment of allicin improves maturation of immature oocytes and subsequent developmental ability of preimplantation embryos

  • Sang-Gi Jeong (a1) (a2), Seung-Eun Lee (a1) (a2), Yun-Gwi Park (a1) (a2), Yeo-Jin Son (a1) (a2), Min-Young Shin (a1) (a2), Eun-Young Kim (a3) (a1) (a2) (a4) and Se-Pill Park (a3) (a1) (a2) (a4)...

Summary

Allicin (AL) regulates the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study investigated the effects of allicin treatment on porcine oocyte maturation and developmental competence. Porcine oocytes were cultured in medium supplemented with 0 (control), 0.01, 0.1, 1, 10 or 100 μM AL, respectively, during in vitro maturation (IVM). The rate of polar body emission was higher in the 0.1 AL-treated group (74.5% ± 2.3%) than in the control (68.0% ± 2.6%) (P < 0.1). After parthenogenetic activation, the rates of cleavage and blastocyst formation were significantly higher in the 0.1 AL-treated group than in the control (P < 0.05). The reactive oxygen species level at metaphase II did not significantly differ among all groups. In matured oocytes, the expression of both BAK and CASP3, and BIRC5 was significantly lower and higher, respectively, in the 0.1 AL-treated group than in the control. Similarly, the expression of BMP15 and CCNB1, and the activity of phospho-p44/42 mitogen-activated protein kinase (MAPK), significantly increased. These results indicate that supplementation of oocyte maturation medium with allicin during IVM improves the maturation of oocytes and the subsequent developmental competence of porcine oocytes.

Copyright

Corresponding author

All correspondence to: Se-Pill Park. Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju Special Self-Governing Province, 63243, Korea. Tel: +82 64 754 4650. E-mail: sppark@jejunu.ac.kr
Eun-Young Kim. Mirae Cell Bio, 288 Achasan-ro, Gwangjin-gu, Seoul, 05066, Korea. Tel: +82 2 457 8758. E-mail: jlokey@daum.net

Footnotes

Hide All
5

These authors contributed equally to this work.

Footnotes

References

Hide All
Albarracin, J.L., Morato, R., Izquierdo, D. & Mogas, T. (2005). Vitrification of calf oocytes: effects of maturation stage and prematuration treatment on the nuclear and cytoskeletal components of oocytes and their subsequent development. Mol. Reprod. Dev. 72, 239–49.
Borlinghaus, J., Albrecht, F., Gruhlke, M.C., Nwachukwu, I.D. & Slusarenko, A.J. (2014). Allicin: chemistry and biological properties. Molecules 19, 12591–618.
Cai, Y., Wang, R., Pei, F. & Liang, B.B. (2007). Antibacterial activity of allicin alone and in combination with β-lactams against Staphylococcus spp. and Pseudomonas aeruginosa . J. Antibiotics 60, 335–8.
Chan, J.Y., Yuen, A.C., Chan, R.Y. & Chan, S.W. (2013). A review of the cardiovascular benefits and antioxidant properties of allicin. Phytother. Res. PTR 27, 637–46.
Chen, S., Tang, Y., Qian, Y., Chen, R., Zhang, L., Wo, L. & Chai, H. (2014). Allicin prevents H2O2-induced apoptosis of HUVECs by inhibiting an oxidative stress pathway. BMC Complement. Altern. Med. 14, 321.
Choi, J.Y., Kang, J.T., Park, S.J., Kim, S.J., Moon, J.H., Saadeldin, I.M., Jang, G. & Lee, B.C. (2013). Effect of 7,8-dihydroxyflavone as an antioxidant on in vitro maturation of oocytes and development of parthenogenetic embryos in pigs. J. Reprod. Dev. 59, 450456.
Chu, Y.L., Ho, C.T., Chung, J.G., Rajasekaran, R. & Sheen, L.Y. (2012). Allicin induces p53-mediated autophagy in Hep G2 human liver cancer cells. J. Agric. Food Chem. 60, 8363–71.
Craig, J., Zhu, H., Dyce, P.W., Petrik, J. & Li, J. (2004). Leptin enhances oocyte nuclear and cytoplasmic maturation via the mitogen-activated protein kinase pathway. Endocrinology 145, 5355–63.
Dedieu, T., Gall, L., Crozet, N., Sevellec, C. & Ruffini, S. (1996). Mitogen-activated protein kinase activity during goat oocyte maturation and the acquisition of meiotic competence. Mol. Reprod. Dev. 45, 351–8.
Duan, X., Wang, Q.C., Chen, K.L., Zhu, C.C., Liu, J. & Sun, S.C. (2015). Acrylamide toxic effects on mouse oocyte quality and fertility in vivo . Sci. Rep. 5, 11562.
Fathi, M., Seida, A.A., Sobhy, R.R., Darwish, G.M., Badr, M.R. & Moawad, A.R. (2014). Caffeine supplementation during IVM improves frequencies of nuclear maturation and preimplantation development of dromedary camel oocytes following IVF. Theriogenology 81, 1286–92.
Gupta, M.K., Uhm, S.J. & Lee, H.T. (2010). Effect of vitrification and β-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization. Fertil. Steril. 93, 2602–7.
Hussein, T.S., Thompson, J.G. & Gilchrist, R.B. (2006). Oocyte-secreted factors enhance oocyte developmental competence. Dev. Biol. 296, 514–21.
Jeon, Y., Kwak, S.S., Cheong, S.A., Seong, Y.H. & Hyun, S.H. (2013). Effect of trans-epsilon-viniferin on in vitro porcine oocyte maturation and subsequent developmental competence in preimplantation embryos. J. Vet Med. Sci. 75, 1277–86.
Kang, J.T., Koo, O.J., Kwon, D.K., Park, H.J., Jang, G., Kang, S.K. & Lee, B.C. (2009). Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells. J. Pineal Res. 46, 22–8.
Kere, M., Siriboon, C., Lo, N.W., Nguyen, N.T. & Ju, J.C. (2013). Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. J. Reprod. Dev. 59, 7884.
Kim, Y.S., Kim, K.S., Han, I., Kim, M.H., Jung, M.H. & Park, H.K. (2012). Quantitative and qualitative analysis of the antifungal activity of allicin alone and in combination with antifungal drugs. PLoS One 7, e38242.
Lee, S.E., Kim, E.Y., Choi, H.Y., Moon, J.J., Park, M.J., Lee, J.B., Jeong, C.J. & Park, S.P. (2014). Rapamycin rescues the poor developmental capacity of aged porcine oocytes. Asian-Aust. J. Anim. Sci. 27, 635–47.
Liang, C.G., Su, Y.Q., Fan, H.Y., Schatten, H. & Sun, Q.Y. (2007). Mechanisms regulating oocyte meiotic resumption: roles of mitogen-activated protein kinase. Mol. Endocrinol. 21, 2037–55.
Liu, S., Jiang, L., Zhong, T., Kong, S., Zheng, R., Kong, F., Zhang, C., Zhang, L. & An, L. (2015). Effect of acrylamide on oocyte nuclear maturation and cumulus cells apoptosis in mouse in vitro . PLoS One 10, e0135818.
Livak, K.J. & Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25, 402–8.
O'Doherty, A.M., O'Gorman, A., al Naib, A., Brennan, L., Daly, E., Duffy, P. & Fair, T. (2014). Negative energy balance affects imprint stability in oocytes recovered from postpartum dairy cows. Genomics 104, 177–85.
Ogawa, B., Ueno, S., Nakayama, N., Matsunari, H., Nakano, K., Fujiwara, T., Ikezawa, Y. & Nagashima, H. (2010). Developmental ability of porcine in vitro matured oocytes at the meiosis II stage after vitrification. J. Reprod. Dev. 56, 356–61.
Phongnimitr, T., Liang, Y., Srirattana, K., Panyawai, K., Sripunya, N., Treetampinich, C. & Parnpai, R. (2013). Effect of l-carnitine on maturation, cryo-tolerance and embryo developmental competence of bovine oocytes. Nihon chikusan Gakkaiho [Anim. Sci. J.] 84, 719–25.
Pu, Y., Wang, Z., Bian, Y., Zhang, F., Yang, P., Li, Y., Zhang, Y., Liu, Y., Fang, F., Cao, H. & Zhang, X. (2014). All-trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation. Nihon chikusan Gakkaiho [Anim. Sci. J.] 85, 833–9.
Sanchez, F. & Smitz, J. (2012). Molecular control of oogenesis. Biochim. Biophys. Acta 1822, 1896–912.
SAS Institute Inc. (2013). Base SASȼç 9.4 Procedures Guide: Statistical Procedures, Second Edition. Cary, North Carolina, USA: SAS Institute Inc.
Shamas-Din, A., Kale, J., Leber, B. & Andrews, D.W. (2013). Mechanisms of action of Bcl-2 family proteins. Cold Spring Harb. Perspect. Biol. 5, a008714.
Siffroi-Fernandez, S., Dulong, S., Li, X.M., Filipski, E., Grechez-Cassiau, A., Peteri-Brunback, B., Meijer, L., Levi, F., Teboul, M. & Delaunay, F. (2014). Functional genomics identify Birc5/survivin as a candidate gene involved in the chronotoxicity of cyclin-dependent kinase inhibitors. Cell Cycle 13, 984–91.
Sun, S.C., Xiong, B., Lu, S.S. & Sun, Q.Y. (2008). MEK1/2 is a critical regulator of microtubule assembly and spindle organization during rat oocyte meiotic maturation. Mol. Reprod. Dev. 75, 1542–8.
Tang, D.W., Fang, Y., Liu, Z.X., Wu, Y., Wang, X.L., Zhao, S., Han, G.C. & Zeng, S.M. (2013). The disturbances of endoplasmic reticulum calcium homeostasis caused by increased intracellular reactive oxygen species contributes to fragmentation in aged porcine oocytes. Biol. Reprod. 89, 124.
Terret, M.E., Lefebvre, C., Djiane, A., Rassinier, P., Moreau, J., Maro, B. & Verlhac, M.H. (2003). DOC1R: a MAP kinase substrate that control microtubule organization of metaphase II mouse oocytes. Development 130, 5169–77.
Tu, G., Zhang, Y.F., Wei, W., Li, L., Zhang, Y., Yang, J. & Xing, Y. (2016). Allicin attenuates H2O2-induced cytotoxicity in retinal pigmented epithelial cells by regulating the levels of reactive oxygen species. Mol. Med. Rep. 13, 2320–6.
Ueno, S., Kurome, M., Ueda, H., Tomii, R., Hiruma, K. & Nagashima, H. (2005). Effects of maturation conditions on spindle morphology in porcine MII oocytes. J. Reprod. Dev. 51, 405–10.
Wang, W., Du, Z., Nimiya, Y., Sukamtoh, E., Kim, D. & Zhang, G. (2016). Allicin inhibits lymphangiogenesis through suppressing activation of vascular endothelial growth factor (VEGF). receptor. J. Nutri. Biochem. 29, 83–9.
Yan, L., Luo, H., Gao, X., Liu, K. & Zhang, Y. (2012). Vascular endothelial growth factor-induced expression of its receptors and activation of the MAPK signaling pathway during ovine oocyte maturation in vitro . Theriogenology 78, 1350–60.
Zhang, W. & Liu, H.T. (2002). MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res. 12, 918.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed