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Transcriptome profile and association study revealed STAT3 gene as a potential quality marker of bovine gametes

Published online by Cambridge University Press:  13 January 2020

Nasser Ghanem Open the ORCID record for Nasser Ghanem [Opens in a new window] ,
Dessie Salilew-Wondim ,
Michael Hoelker ,
Karl Schellander  and
Dawit Tesfaye Open the ORCID record for Dawit Tesfaye [Opens in a new window]
Nasser Ghanem
Affiliation:
Animal Production Department, Faculty of Agriculture, Cairo University, Giza, Egypt
Dessie Salilew-Wondim
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany
Michael Hoelker
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany
Karl Schellander
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany
Dawit Tesfaye*
Affiliation:
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115Bonn, Germany Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory (ARBL), Colorado State University, Fort Collins, CO, USA
*
Author for correspondence: Dawit Tesfaye, Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory (ARBL), Colorado State University, 1351 Rampart Rd, Fort Collins, CO, USA. Tel: +1 970 491 891. E-mail: Dawit.Tesfaye@colostate.edu
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Summary

The present study was aimed to investigate differences in molecular signatures in oocytes derived from Holstein-Friesian heifers with different genetic merit for fertility, euthanized during day 0 or day 12 of the estrous cycle. Moreover, association between single nucleotide polymorphisms (SNPs) of ODC1 and STAT3 genes and bull fertility traits was investigated. The gene expression patterns were analyzed using cDNA array and validated with quantitative real-time polymerase chain reaction (PCR). The result revealed that several genes have shown not only to be regulated by fertility merit but also by the day of oocyte recovery during the estrous cycle. The STAT3 gene was found to be upregulated in oocytes recovered from animals with high fertility merit at both day 0 and day 12. Some other genes like PTTG1, ODC1 and TUBA1C were downregulated at day 0 and upregulated at day 12 in high, compared with low, fertility merit recovered oocytes. In contrast, the transcript abundance of TPM3 was upregulated at day 0 and downregulated at day 12 in high, compared with low, fertility merit recovered oocytes. In addition, ODC1 and STAT3 were found to be associated (P < 0.05) with sperm quality traits as well as flow cytometry parameters. Therefore, the expression of several candidate genes including ODC1 and STAT3 was related to the genetic merit of the cow. In addition polymorphisms in these two genes were found to be associated with bull semen quality.

Keywords

Developmental competenceGene expressionOocyteSperm
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 116 - 130
Copyright
© Cambridge University Press 2020

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