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Transcription of paternal Y-linked genes in the human zygote as early as the pronucleate stage

Published online by Cambridge University Press:  26 September 2008

Asangla Ao ,
Robert P. Erickson ,
Robert M.L. Winston  and
Alan H Handysude
Asangla Ao*
Affiliation:
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, UK, and Department of Pediatrics, The University of Arizona, Tucson, USA
Robert P. Erickson
Affiliation:
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, UK, and Department of Pediatrics, The University of Arizona, Tucson, USA
Robert M.L. Winston
Affiliation:
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, UK, and Department of Pediatrics, The University of Arizona, Tucson, USA
Alan H Handysude
Affiliation:
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, UK, and Department of Pediatrics, The University of Arizona, Tucson, USA
*
A. Ao, phD, Institute of Obstetrics and Gynaecology, Roly postgraduate Medical shool, Hommersmith Hospital, Du Cane Road, London W12 ONN, UK. Telephone: 081-743-2030, ext 2084. Fax: 081-749-6973.
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Summary

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Global activation of the embryonic genome occurs at the 4– to 8–cell stage in human embryos and is marked by continuation of early cleavage divisions in the presence of transcriptional inhibitors. Here we demonstrate, using recerse transcripase–polymerase chin reaction (Rt–PCR), the presence of transcripts for wo paternal Y chromosomal genes, ZFY and SRY in human preimplantation embryos. ZFY transcripts were detected as early as the pronucleate stage, 20–24 h post-insemination In vitro and at intermediate stages up to the blastocyst stage. SRY Transcripts were also detected at 2–cell to blastocyos observed in many mammalian species focuses attention on the role of events in six determination prior to gonad differentiation.

Keywords

Preimplantation human embryoRT-PCRsex determinationSRYZFY
Type
Article
Information
Zygote , Volume 2 , Issue 4 , November 1994 , pp. 281 - 287
Copyright
Copyright © Cambridge University Press 1994

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