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Somatic nucleus remodelling in immature and mature Rassir oocyte cytoplasm

  • Li-Sheng Zhang (a1), Ke-Ying Zhang (a1), Li-Juan Yao (a1), Shu-Zhen Liu (a1), Cai-Xia Yang (a1), Zhi-Sheng Zhong (a1), Yue-Liang Zheng (a1), Qing-Yuan Sun (a1) and Da-Yuan Chen (a1)...

Abstract

Successful production of cloned animals derived from somatic cells has been achieved in sheep, cattle, goats, mice, pigs, rabbits, etc. But the efficiency of nuclear transfer is very low in all species. The present study was conducted to examine somatic nucleus remodelling and developmental ability in vitro of rabbit embryos by transferring somatic cells into enucleated germinal vesicle (GV), metaphase I (MI) or metaphase II (MII) oocytes. Microtubules were organized around condensed chromosomes after the nucleus had been transferred into any of the three types of cytoplasm. A bipolar spindle was formed in enucleated MII cytoplasm. Most of the nuclei failed to form a normal spindle within GV and MI cytoplasm. Some chromosomes scattered throughout the cytoplasm and some formed a monopolar spindle. Pseudopronucleus formation was observed in all three types of cytoplasm. Reconstructed embryos with MI and MII cytoplasm could develop to blastcysts. Nuclei in GV cytoplasm could develop only to the 4-cell stage. These results suggest that (1) GV material is important for nucleus remodelling after nuclear transfer, and (2) oocyte cytoplasm has the capacity to dedifferentiate somatic cells during oocyte maturation.

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Corresponding author

All correspondence to: Da-Yuan Chen, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Bei Si Huan Xi Lu, Haidian, Beijing 100080, China. Tel: +8610 62560528, 62562793. Fax: +8610 62565689. e-mail: chendy@panda.ioz.ac.cn

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