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Seminal quality comparison of first and second cryopreserved ejaculates of Alpine kid goats by flow cytometry

Published online by Cambridge University Press:  17 November 2022

Marco Aurélio Schiavo Novaes Open the ORCID record for Marco Aurélio Schiavo Novaes [Opens in a new window] ,
Palloma Porto Almeida ,
Domingos Lollobrigida de Souza Netto ,
Victor Hugo Rabelo de Carvalho ,
João Vitor Ribeiro Lovatti ,
Joana Diniz da Silveira ,
Giulia Berzoini Costa Leite  and
Ciro Alexandre Alves Torres
Marco Aurélio Schiavo Novaes*
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Animal Reproduction Laboratory, 36570-900, Viçosa, MG, Brasil
Palloma Porto Almeida
Affiliation:
Department of General Biology, Universidade Federal de Viçosa, Immunochemistry and Glycobiology Laboratory, 36570-900, Viçosa, MG, Brasil
Domingos Lollobrigida de Souza Netto
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Animal Reproduction Laboratory, 36570-900, Viçosa, MG, Brasil
Victor Hugo Rabelo de Carvalho
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Animal Reproduction Laboratory, 36570-900, Viçosa, MG, Brasil
João Vitor Ribeiro Lovatti
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Animal Reproduction Laboratory, 36570-900, Viçosa, MG, Brasil
Joana Diniz da Silveira
Affiliation:
Department of Veterinary Medicine, Universidade Federal de Viçosa, Veterinary Clinical Pathology Research Laboratory, 36570-900, Viçosa, MG, Brasil
Giulia Berzoini Costa Leite
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Animal Reproduction Laboratory, 36570-900, Viçosa, MG, Brasil
Ciro Alexandre Alves Torres
Affiliation:
Department of Animal Science, Universidade Federal de Viçosa, Animal Reproduction Laboratory, 36570-900, Viçosa, MG, Brasil
*
Author for correspondence: Marco Aurélio Schiavo Novaes. 35 Magnólia St, 306 Apartment, Fortaleza, CE, Brazil. Tel: +55 31994778651. E-mail: marcoaurelioschiavo@gmail.com
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Summary

Discarding the first ejaculate is recommended as an alternative for improving seminal quality after long sexual resting, especially when semen should be used for cryopreservation. However, when the males are not in sexual resting the necessity to discarding the first ejaculate is still unknown. Therefore, this study aimed to compare by flow cytometry the quality of the first and second ejaculates. Ten kids and uniform goats between 5 and 6 months of age were used in a completely randomized design. Semen collection was carried out every 4 days, until a total of five ejaculates per animal in each treatment was completed. The fresh and frozen semen collected were processed and analyzed using macroscopic and microscopic parameters, resistance test, hypo-osmotic medium test, and flow cytometry (FC). The FC parameters were production of reactive oxygen species, plasma and acrosomal membrane integrity, and lipid peroxidation of the plasma membrane. The ejaculates did not differ for the resistance test, the reactivity in the hypo-osmotic medium and for the macroscopic and microscopic seminal parameters, except for sperm volume and concentration. The first ejaculate had a higher percentage of minor and total defects. None of the FC parameters analyzed differed between the first and second ejaculates. The first and second ejaculates demonstrated similar seminal qualities, so for Alpine kid goats without a sexual resting period, discarding the first ejaculate it is not recommended.

Keywords

CryopreservationEjaculate orderFlow cytometryLipid peroxidationROS
Type
Research Article
Information
Zygote , Volume 31 , Issue 1 , February 2023 , pp. 62 - 69
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Copyright
© The Author(s), 2022. Published by Cambridge University Press

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References

Agossou, D. J. and Koluman, N. (2018). An objective analysis of factors affecting buck semen quality attributes during cryopreservation: A mini review. Annual Research and Review in Biology, 27(3), 1–7. doi: 10.9734/ARRB/2018/42087 CrossRefGoogle Scholar
Agricultural and Food Research Council (AFRC). (1998). The Nutrition of Goats. Technical Committee on Response to Nutrients. Report Number 10. CAB International, Wallingford.Google Scholar
Aitken, R. J., Wingate, J. K., De Iuliis, G. N. and McLaughlin, E. A. (2007). Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Molecular Human Reproduction, 13(4), 203–211. doi: 10.1093/molehr/gal119 CrossRefGoogle ScholarPubMed
Anand, M. and Yadav, S. (2016). Assessment of motion and kinematic characteristics of frozen–thawed Sirohi goat semen using computer-assisted semen analysis. Veterinary World, 9(2), 203–206. doi: 10.14202/vetworld.2015.203-206 CrossRefGoogle ScholarPubMed
Ayala, A., Muñoz, M. F. and Argüelles, S. (2014). Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 2014, 360438. doi: 10.1155/2014/360438 CrossRefGoogle ScholarPubMed
Azevedo, H. C., Machado, R., Simplício, A. A. and Soares, A. (2000). Características do sêmen caprino congelado: influência do tipo de palheta e concentração espermática. Revista Científica Rural, 5, 148–157.Google Scholar
Bagis, S., Tamer, L., Sahin, G., Bilgin, R., Guler, H., Ercan, B. and Erdogan, C. (2005). Free radicals and antioxidants in primary fibromyalgia: An oxidative stress disorder? Rheumatology International, 25(3), 188–190. doi: 10.1007/s00296-003-0427-8 CrossRefGoogle ScholarPubMed
Barkawi, A. H., Elsayed, E. H., Ashour, G. and Shehata, E. (2006). Seasonal changes in semen characteristics, hormonal profiles and testicular activity in Zaraibi goats. Small Ruminant Research, 66(1–3), 209–213. doi: 10.1016/j.smallrumres.2005.09.007 CrossRefGoogle Scholar
Basiji, D. A., Ortyn, W. E., Liang, L., Venkatachalam, V. and Morrissey, P. (2007). Cellular image analysis and imaging by flow cytometry. Clinics in Laboratory Medicine, 27(3), 653–670. doi: 10.1016/j.cll.2007.05.008 CrossRefGoogle ScholarPubMed
Baumber, J., Ball, B. A., Gravance, C. G., Medina, V. and Davies-Morel, M. C. G. (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. Journal of Andrology, 21(6), 895–902.Google ScholarPubMed
Bezerra, F. Q. G., Aguiar Filho, C. R., Freitas Neto, L. M., Santos Junior, E. R., Chaves, R. M., Azevedo, E. M. P., Santos, M. H. B., Lima, P. F. and Oliveira, M. A. L. (2009). Body weight, scrotal circumference and testosterone concentration in young Boer goat males born during the dry or rainy seasons. South African Journal of Animal Science, 39(4), 301–306.Google Scholar
Bittencourt, R. F., Ribeiro Filho, A. L., Santos, A. D. F., Chalhoub, M., Alves, S. G. G., Vasconcelos, M. F., Leandro, E. E. S. and Guimarães, J. D. (2005). Use of hipoosmotic test to evaluate the efficacy of different protoclols of cryopreservation of the goat semen. Ciência Animal Brasileira, 6(3), 213–218.Google Scholar
Borg, K., Colenbrander, B., Fazeli, A., Parlevliet, J. and Malmgren, L. (1997). Influence of thawing method on motility, plasma membrane integrity and morphology of frozen–thawed stallion spermatozoa. Theriogenology, 48(4), 531–536. doi: 10.1016/s0093-691x(97)00269-0 CrossRefGoogle ScholarPubMed
CBRA (Colégio brasileiro de reprodução animal). (2013). Manual para exame andrológico e avaliação de sêmen animal (3rd edn). Belo Horizonte, Brazil.Google Scholar
Cross, N. L., Morales, P., Overstreet, J. W. and Hanson, F. W. (1986). Two simple methods for detecting acrosome-reacted human sperm. Gamete Research, 15(3), 213–226. doi: 10.1002/mrd.1120150303 CrossRefGoogle Scholar
Del Rio, D., Stewart, A. J. and Pellegrini, N. (2005). A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, Metabolism, and Cardiovascular Diseases, 15(4), 316–328. doi: 10.1016/j.numecd.2005.05.003 CrossRefGoogle ScholarPubMed
Dias, J. C. O., Veloso, C. M., Santos, M. CdR., Oliveira, C. T. S. A. Md, Silveira, C. O., Iglesias, E., Maitan, P. P. and Sanglard, L. M. P. (2017). Seasonal variation in the reproductive activity of male goats raised under tropical climate conditions. Revista Brasileira de Zootecnia, 46(3), 192–201. doi: 10.1590/s1806-92902017000300003 CrossRefGoogle Scholar
Ebenezer, R. J., Paulpandi, T. G., Siva Kumar, T., Gopinathan, A. and Meenakshi Sundaram, S. (2021). Supplementation of the diets with hydroponic maize fodder affects digestibility, puberty, sexual behavior, and semen characteristics in buck kids. Tropical Animal Health and Production, 53(2), 310. doi: 10.1007/s11250-021-02761-9 CrossRefGoogle ScholarPubMed
Falchi, L., Pau, S., Pivato, I., Bogliolo, L. and Zedda, M. T. (2020). Resveratrol supplementation and cryopreservation of buck semen. Cryobiology, 95, 60–67. doi: 10.1016/j.cryobiol.2020.06.005 CrossRefGoogle ScholarPubMed
Firman, R. C., Young, F. J., Rowe, D. C., Duong, H. T. and Gasparini, C. (2015). Sexual rest and post-meiotic sperm ageing in house mice. Journal of Evolutionary Biology, 28(7), 1373–1382. doi: 10.1111/jeb.12661 CrossRefGoogle ScholarPubMed
Garcia, A. R. (2004). [Effects of testicular heat stress and the use of recombinant bovine somatotropin on seminal characteristics, membrane integrity, mitochondrial function and chromatin structure of spermatozoa from Simmental bulls (Bos taurus taurus).] The Digital Library of Theses and Dissertations of the University of São Paulo.Google Scholar
Gibbons, A., Cueto, M. and Wolff, M. (2009). Artificial insemination in goats. National Institute of Agricultural Technology. Available from: http://cac-program.org/files/fiber/pub_Eng_Artificial_Insemintion_in_Goats.pdf Google Scholar
Gillan, L., Evans, G. and Maxwell, W. M. C. (2005). Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology, 63(2), 445–457. doi: 10.1016/j.theriogenology.2004.09.02 CrossRefGoogle ScholarPubMed
Gopinathan, A., Selvan, A. S., Jawhar, K. T. P. and Karthickeyan, S. M. K. (2021). Influence of nongenetic factors on semen characteristics of Malabari bucks. Indian Journal of Small Ruminants 27(1), 56–59.CrossRefGoogle Scholar
Hafez, E. (1995). Reprodução animal (6th edn). Manole: São Paulo.Google Scholar
Hervieu, J., Morand-Fehr, P., Schmidely, P. H., Fedele, V. and Delfa, R. (1991). Body measurements explaining variations in scores for the sternal, lumbar, and caudal regions used to estimate body condition in dairy goats. Options Méditerranéennes-Série Séminaires, 13, 43–56.Google Scholar
Hossain, M. S., Johannisson, A., Wallgren, M., Nagy, S., Siqueira, A. P. and Rodriguez-Martinez, H. (2011) Flow cytometry for the assessment of animal sperm integrity and functionality: State of the art. Asian Journal of Andrology, 13, 406–419.CrossRefGoogle ScholarPubMed
Jiménez-Rabadán, P., Ramón, M., García-Álvarez, O., Maroto-Morales, A., Del Olmo, E., Pérez-Guzmán, M. D., Bisbal, A. J., Fernández-Santos, M. R., Garde, J. J. and Soler, A. J. (2012). Effect of semen collection method (artificial vagina vs. electroejaculation), extender and centrifugation on post-thaw sperm quality of Blanca-Celtibérica buck ejaculates. Animal Reproduction Science, 132(1–2), 88–95. doi: 10.1016/j.anireprosci.2012.04.005 CrossRefGoogle ScholarPubMed
Kastelic, J. P. and Thundathil, J. C. (2008). Breeding soundness evaluation and semen analysis for predicting bull fertility. Reproduction in Domestic Animals, 43, Suppl. 2, 368–373. doi: 10.1111/j.1439-0531.2008.01186.x CrossRefGoogle Scholar
Kumbhar, U. B., Gulavane, S. U., Gaikwad, S. M., Shelar, R. R., Deshpande, V. P., Rebeiro, R. and Yadav, K. (2019). Correlation of testicular ultrasonography, testicular biometry, serum testosterone levels and seminal attributes in pre and post-pubertal age for breeding soundness evaluation in Osmanabadi bucks. Tropical Animal Health and Production, 51(6), 1467–1480. doi: 10.1007/s11250-019-01834-0 CrossRefGoogle ScholarPubMed
Martins, L. F., Pereira, M. C. B., Guimarães, J. D., da Costa, E. Pd, Silveira, TdS., Torres, C. A. A., Rodrigues, M. T. and Braz, V. B. (2006) Avaliação espermática e da concentração de proteínas solúveis no plasma seminal de bodes da raça Alpina em regime de monta controlada. Revista Brasileira de Zootecnia. College Art Association. MT, 35(4), Suppl., 1653–1659. doi: 10.1590/S1516-35982006000600011 CrossRefGoogle Scholar
Mátyus, L., Szabó, G., Resli, I., Gáspár, R. and Damjanovich, S. (1984). Flow cytometric analysis of viability of bull sperm cells. Acta Biochimica et Biophysica; Academiae Scientiarum Hungaricae, 19(3–4), 209–214.Google ScholarPubMed
Mayorga-Torres, J. M., Agarwal, A., Roychoudhury, S., Cadavid, A. and Cardona-Maya, W. D. (2016). Can a short term of repeated ejaculations affect seminal parameters? Journal of Reproduction and Infertility, 17(3), 177–183.Google Scholar
Mojapelo, M. M. and Lehloenya, K. C. (2019). Effect of selenium supplementation on attainment of puberty in Saanen male goat kids. Theriogenology, 138, 9–15. doi: 10.1016/j.theriogenology.2019.06.044 CrossRefGoogle ScholarPubMed
Morrell, J. M., Malaluang, P., Ntallaris, T. and Johannisson, A. (2022). Practical method for freezing buck semen. Animals: an Open Access Journal from MDPI, 12(3), 352. doi: 10.3390/ani12030352 CrossRefGoogle ScholarPubMed
Neild, D. M., Brouwers, J. F. H. M., Colenbrander, B., Agüero, A. and Gadella, B. M. (2005). Lipid peroxide formation in relation to membrane stability of fresh and frozen thawed stallion spermatozoa. Molecular Reproduction and Development, 72(2), 230–238. doi: 10.1002/mrd.20322 CrossRefGoogle ScholarPubMed
Nöthling, J. O. and Irons, P. C. (2008). A simple multidimensional system for the recording and interpretation of sperm morphology in bulls. Theriogenology, 69(5), 603–611. doi: 10.1016/j.theriogenology.2007.11.007 CrossRefGoogle ScholarPubMed
Olivares, C. C. S., da Fonseca, J. F., de Almeida Camargo, L. S., de Souza-Fabjan, J. M. G., Rodrigues, A. L. R. and Brandão, F. Z. (2015). Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. Small Ruminant Research, 127, 44–49. doi: 10.1016/j.smallrumres.2015.04.009 CrossRefGoogle Scholar
Oliveira, I. R. Sd, Alves, H. M., Castelo, T. S., Bezerra, F. S. B., Bezerra, A. C. D. S. and Silva, A. R. (2013). Correlações entre o teste hiposmótico e a avaliação clássica do sêmen de caprinos. Ciência Animal Brasileira, 14(2), 216–221. doi: 10.5216/cab.v14i2.12921 CrossRefGoogle Scholar
Ozkavukcu, S., Erdemli, E., Isik, A., Oztuna, D. and Karahuseyinoglu, S. (2008). Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. Journal of Assisted Reproduction and Genetics, 25(8), 403–411. doi: 10.1007/s10815-008-9232-3 CrossRefGoogle ScholarPubMed
Santos, A. D. F., Torres, C. A. A., Fonseca, J. F., Borges, A. M., Rovay, H., Goretti, R. G., Guimarães, J. D., Costa, E. P., Barbosa, L. P., Maffili, V. V. and Fraga, D. B. M. (2001) Uso do Teste Hiposmótico (HOST) para avaliar a congelabilidade do sêmen de caprinos das raças Alpina e Saanen, jovens e adultos, submetidos ao manejo com luz artificial. Revista Brasileira de Reprodução Animal, 25, 438–439.Google Scholar
Santos, A. D. F., Torres, C. A. A., Fonseca, J. Fd, Borges, Á. M., Guimarães, J. D., Pd, da Costa, E. and Rovay, H. (2006) Uso de testes complementares para avaliação do congelamento do sêmen de bodes submetidos ao manejo de fotoperíodo artificial. Revista Brasileira de Zootecnia, 35(5), 1934–1942. doi: 10.1590/S1516-35982006000700008 CrossRefGoogle Scholar
Shamsuddin, M., Amiri, Y. and Bhuiyan, M. M. U. (2000). Characteristics of buck semen with regard to ejaculate numbers, collection intervals, diluents and preservation periods. Reproduction in Domestic Animals, 35(2), 53–57. doi: 10.1046/j.1439-0531.2000.00199.x CrossRefGoogle Scholar
Squires, E. L., Pickett, B. W., Graham, J. K., Vanderwall, D. K., McCue, P. M. and Brummer, J. E. (1999). Cooled and frozen stallion semen. Animal Reproduction and Biotechnology Laboratory, Fort Collins.Google Scholar
Trejo, G., Marquez, M., Salazar, C., Felipe, S., Garcia, L. and Reyes, R. (1988). Testis growth, seminal quality, sperm reserves and testosterone production in Alpine kids treated hormonally around puberty. 11th International Congress on Animal Reproduction and Artificial Insemination, Dublin, June 26–30, 1988.Google Scholar
Turri, F., Madeddu, M., Gliozzi, T. M., Gandini, G. and Pizzi, F. (2016). Relationship between body weight, sexual secondary traits and epididymal semen quality in the Alpine goat. Small Ruminant Research, 135, 81–84. doi: 10.1016/j.smallrumres.2015.12.017 CrossRefGoogle Scholar
Valença, R. M. B., Guerra, M. M. P. and Guerra, P. (2007). Espécies Reativas ao Oxigênio (ROS) e a utilização de antioxidantes na criopreservação do sêmen suíno. Revista Brasileira de Reprodução Animal, 31, 47–53.Google Scholar
Verstegen, J., Iguer-Ouada, M. and Onclin, K. (2002). Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57(1), 149–179. doi: 10.1016/s0093-691x(01)00664-1 CrossRefGoogle ScholarPubMed
Watson, P. F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60–61, 481–492. doi: 10.1016/s0378-4320(00)00099-3 CrossRefGoogle ScholarPubMed