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Participation of inositol trisphosphate and ryanodine receptors in Bufo arenarum oocyte activation

Published online by Cambridge University Press:  30 September 2010

M.T. Ajmat ,
F. Bonilla ,
L. Zelarayán  and
M.I. Bühler
M.T. Ajmat
Affiliation:
Departamento de Biología del Desarrollo (INSIBIO), Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
F. Bonilla
Affiliation:
Instituto de Biología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
L. Zelarayán
Affiliation:
Departamento de Biología del Desarrollo (INSIBIO), Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
M.I. Bühler*
Affiliation:
Departamento de Biología del Desarrollo (INSIBIO), Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
*
All correspondence to Marta I. Bühler. Departamento de Biología del Desarrollo (INSIBIO), Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina. Fax: +54 381 4248025. e-mail: mbuhler@fbqf.unt.edu.ar
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Summary

Calcium is considered the most important second messenger at fertilization. Transient release from intracellular stores is modulated through both agonist-gated channels, IP3Rs and RyRs, which can be found individually or together depending on the oocyte species. Using the four commonly used compounds (thimerosal, caffeine, heparin and ruthenium red), we investigated the existence and interdependence of both IP3Rs and RyRs in mature Bufo arenarum oocytes. We found that caffeine, a well known specific RyRs agonist, was able to trigger oocyte activation in a dose-dependent manner. Microinjection of 10 mM caffeine showed 100% of oocytes exhibiting characteristic morphological criteria of egg activation. Ruthenium red, the specific RyR blocker, was able to inhibit oocyte activation induced either by sperm or caffeine. Our present findings provide the first reported evidence of the existence of RyR in frogs. We further explored the relationship between IP3Rs and RyRs in B. arenarum oocytes by exposing them to the agonists of one class after injecting a blocker of the other class of receptor. We found that thimerosal overcame the inhibitory effect of RyR on oocyte activation, indicating that IP3Rs function as independent receptors. In contrast, previous injection of heparin delayed caffeine-induced calcium release, revealing a relative dependence of RyRs on functional IP3Rs, probably through a CICR mechanism. Both receptors play a role in Ca2+ release mechanisms although their relative contribution to the activation process is unclear.

Keywords

AmphibianIP3 receptorOocyte activationRyanodine receptor
Type
Research Article
Information
Zygote , Volume 19 , Issue 2 , May 2011 , pp. 171 - 180
Copyright
Copyright © Cambridge University Press 2010

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