Skip to main content Accessibility help
×
Home

Micromorphological and ultrastructural description of spermatozoa from squirrel monkeys (Saimiri collinsi Osgood, 1916)

  • Danuza L. Leão (a1) (a2), Wlaisa V. Sampaio (a1) (a2) (a3), Patrícia C. Sousa (a1), Arlindo A.A. Moura (a4), Irma C. Oskam (a5) (a6), Regiane R. Santos (a1) and Sheyla F.S. Domingues (a1) (a2) (a5) (a6)...

Summary

Saimiri collinsi is used as an animal model in biotechnology research for conservation of species from the genus Saimiri. However, the development of biotechnologies depends on a proper knowledge of the sperm morphology to understand the basic aspects of sperm physiology, as potential male fertility depends on different cellular sperm structures. With this purpose, this study characterized the micromorphological and ultrastructural characteristics of squirrel monkeys (Saimiri collinsi) sperm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM electromyography revealed that a normal Saimiri collinsi sperm measures 71.7 ± 0.7 μm with lateral tail insertion, a paddle-shaped flattened head and an acrosome occupying most of the head. TEM also showed that the middle piece is characterized by a central 9 + 2 microtubule axoneme surrounded by nine dense fibres, and that the mitochondria were juxtaposed, forming the mitochondrial sheath. Here we provide the first micromorphological and ultrastructure description of S. collinsi sperm.

Copyright

Corresponding author

Author for correspondence: Danuza Leite Leão. Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, BR 316 Km 61, CEP 68740-970, Castanhal, Pará, Brazil. Tel: +55 91 33114707. E-mail: danleao.88@gmail.com

References

Hide All
Almeida, DVC, Lima, JS, Leão, DL, Oliveira, KG, Santos, RR, Domingues, SFS and Miranda, MS (2018) The effects of Trolox on the quality of sperm from captive squirrel monkey during liquefaction in the extender ACP-118™. Zygote 26, 333–35.
Arakaki, PR, Carvalho, FM, Castro, PHG, Muniz, JAPC and Valle, RDRD (2017) Collection, evaluation, and coagulum dissolution of semen from Goeldi’s monkey, Callimico goeldii. Folia Primatol 88, 334–43.
Arakaki, PR, Nichi, M, Monteiro, FOB, Muniz, JAPC, Guimarães, MABV and Valle, RDRD (2019) Comparison of semen characteristics and sperm cryopreservation in common marmoset (Callithrix jacchus) and black-tufted-ear marmoset (Callithrix penicillata). J Med Primatol 48, 3242.
Baccetti, B, Capitani, S, Collodel, G, Di Cairano, G, Gambera, L, Moretti, E and Piomboni, P (2001) Genetic sperm defects and consanguinity. Hum Reprod 16, 1365–71.
Bartoov, B, Eltes, F, Weissenberg, R and Lunenfeld, B (1980) Morphological characterization of abnormal human spermatozoa using transmission electron microscopy. Arch Androl 5, 305–22.
Bedford, JM and Nicander, L (1971) Ultrastructural changes in the acrosome and sperm membranes during maturation of spermatozoa in the testis and epididymis of the rabbit and monkey. J Anat 108, 527–43.
Bezerra, LGP, Souza, ALP, Silva, HVR, Vasconcelos, FR, Moura, AAA, Pereira, AF, Oliveira, MF and Silva, AR (2018) Ultrastructural description of fresh and frozen/thawed sperm derived from collared peccaries (Pecari tajacu Linnaeus, 1758). Microscop Res Tech 81, 19.
Bloom, E (1973) The ultrastructure of some characteristic sperm defects and a proposal for a new classification of the bull spermogram. Nord Vet 25, 8391.
Bornens, M (2012) The centrosome in cells and organisms. Science 335, 422–26.
Gomendio, M and Roldan, ER (1991) Sperm competition influences sperm size in mammals. Proc Biol Sci 243, 181–5.
Gould, KG (1980) Scanning electron microscopy of the primate sperm. Int Ver Cytol 63, 323–55.
Gould, KG and Martin, DE (1978) Comparative morphology of primate spermatozoa using scanning electron microscopy. II. Families Cercopithecidae, Lorisidae, Lemuridae. J Hum Evol 7, 637–42.
Humphries, S, Evans, JP and Simmons, LW (2008) Sperm competition: linking form to function. BMC Evol Biol 8, 319.
IUCN (2019) The IUCN Red List of Threatened Species. Version 2019-2. http://www.iucnredlist.org. Accessed 20 August 2019.
Leão, DL, Miranda, AS, Brito, AB, Lima, JS, Santos, RR and Domingues, SFS (2015) Efficacious long-term cooling and freezing of Sapajus apella semen in ACP-118®. Anim Reprod Sci 159, 118–23.
López-Armengol, MF, Jurado, SB, Pelufo, V and Aisen, EG (2012) A quantitative ultramorphological approach for systematic assessment of sperm head regions: an example in rams. Cryobiology 64, 223–34.
Martin, DE, Gould, KG and Warner, H (1975) Comparative morphology of primate spermatozoa using scanning electron microscopy. I. Families Hominidae, Pongidae, Cercopithecidae and Cebidae. J Hum Evol 4, 287–92.
Nakazato, C, Yoshizawa, M, Isobe, K, Kusakabe, KT, Kuraishi, T, Hattori, S, Matsumoto, H, Fukui, E, Kuwahata, A, Ochi, M, Kiso, Y and Kai, C (2015) Morphological characterization of spermatozoa of the night monkey. J Mamm Ova Res 31, 3740.
Nussdorfer, P, Cilenšek, I, Zorn, B and Petrovič, D (2018) Adapted methods for scanning electron microscopy (SEM) in assessment of human sperm morphology. Bosn J Basic Med Sci 18, 43–8.
Oliveira, KG, Leão, DL, Almeida, DVC, Santos, RR and Domingues, SFS (2015) Seminal characteristics and cryopreservation of sperm from the squirrel monkey, Saimiri collinsi. Theriogenology 84, 743–9.
Oliveira, KG, Santos, RR, Leão, DL, Brito, AB, Lima, JS, Sampaio, WV and Domingues, SFS (2016a) Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species. Cryobiology 72, 283–9.
Oliveira, KG, Santos, RR, Leão, DL, Queiroz, HL, Paim, FP, Vianez-Júnior, JLSG and Domingues, SFS (2016b) Testicular biometry and semen characteristics in captive and wild squirrel monkey species (Saimiri sp.). Theriogenology 86, 879–87.
Ozkavukcu, S, Erdemli, E, Ayca Isik, A, Oztuna, D and Karahuseyinoglu, S (2008) Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet 25, 403–11.
Pesch, S, Bostedt, H, Failing, K and Bergmannc, M (2006) Advanced fertility diagnosis in stallion semen using transmission electron microscopy. Anim Reprod Sci 91, 285–98.
Sampaio, WV, Oliveira, KG, Leão, DL, Caldas-Bussiere, MC, Queiroz, HL, Paim, HP, Santos, RR and Domingues, SFS (2017) Morphologic analysis of sperm from two neotropical primate species: comparisons between the squirrel monkeys Saimiri collinsi and Saimiri vanzolinii. Zygote 25, 18.
Silva, HVR, Nunes, TGP, Ribeiro, LR, Freitas, LA, Oliveira, MF, Assis Neto, AC, Silva, AR and Silva, LDM (2019) Morphology, morphometry, ultrastructure, and mitochondrial activity of jaguar (Panthera onca) sperm. Anim Reprod Sci 203, 8493.
Sousa, PC, Santos, EAA, Bezerra, JAB, Lima, GL, Castelo, TS, Fontenele-Neto, JD, Silva, AR (2013) Morphology, morphometry and ultrastructure of captive six-banded armadillo (Euphractus sexcinctus) sperm. Anim Reprod Sci 140, 279–85.
Steinberg, ER, Nieves, M, Ascunce, MS, Palermo, AM and Mudry, MD (2009) Morphological and genetic characterization of Saimiri boliviensis. Int J Primatol 30, 2941.
Swanson, WF, Valle, RR, Carvalho, FM, Arakaki, PR, Rodas-Martínez, AZ, Muniz, JAPC and García-Herreros, M (2016) Sperm morphology assessment in captive neotropical primates. Reprod Dom Anim 51, 623–7.
Visco, V, Raffa, S, Elia, J, Delfino, M, Imbrogno, N, Torrisi, MR and Mazzilli, F (2010) Morphological sperm defects analyzed by light microscopy and transmission electron microscopy and their correlation with sperm motility. Int J Urol 17, 259–66.
WHO (2010) Laboratory Manual for the Examination and Processing of Human Semen, 5th edn. Geneva, Switzerland: WHO Press.
Zamboni, L, Zemjanis, R and Stefanini, M (1971) The fine structure of monkey and human spermatozoa. Anat Rec 169, 129–53.

Keywords

Micromorphological and ultrastructural description of spermatozoa from squirrel monkeys (Saimiri collinsi Osgood, 1916)

  • Danuza L. Leão (a1) (a2), Wlaisa V. Sampaio (a1) (a2) (a3), Patrícia C. Sousa (a1), Arlindo A.A. Moura (a4), Irma C. Oskam (a5) (a6), Regiane R. Santos (a1) and Sheyla F.S. Domingues (a1) (a2) (a5) (a6)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.