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Low salinity negatively affects early larval development of Nile tilapia, Oreochromis niloticus: insights from skeletal muscle and molecular biomarkers

  • Luis Henrique Melo (a1), Yuri Simões Martins (a1), Rafael Magno Costa Melo (a1), Paula Suzanna Prado (a1), Ronald Kennedy Luz (a2), Nilo Bazzoli (a1) (a3) and Elizete Rizzo (a1)...

Summary

The present study evaluated the effects of low salinity on the early larval development of Oreochromis niloticus, specifically histological damage to white muscle, morphology of the yolk-sac surface and trunk area, and molecular expression of apoptosis and cell proliferation biomarkers. Newly hatched larvae were submitted to four salinity treatments for a period of 48 or 72 h, in duplicate: (S0) freshwater, (S2) 2 g l−1, (S4) 4 g l−1, and (S6) 6 g l−1NaCl. Larval development was examined using histology, electron microscopy, enzyme-linked immunosorbent assay (ELISA), and morphometry. At the yolk-sac surface, larvae of S4 and S6 displayed alterations to the apical opening of chloride cells that may be related to osmotic expenditure caused by the increased salinity. Caspase-3 expression did not differ significantly among treatments, however significantly lower proliferating cell nuclear antigen (PCNA) expression (P < 0.05) suggested minor cell proliferation in larvae of S4 and S6 compared with S0 and S2. Furthermore, there was a significant reduction in both trunk area and percentage of normal white muscle fibres (WF) in larvae of S4 and S6. Vacuolated areas and myofibrils concentrated at the cell periphery and found in the white muscle from larvae exposed to saline environments suggested disturbance to muscle development. Oedema and mononuclear infiltrate were also observed in the white muscle of S4 and S6 larvae. Together these results indicated that treatments with 4 and 6 g l−1 NaCl may cause osmoregulation expenditure, morphological alterations to the yolk-sac surface and histological damage to skeletal muscle that negatively affected the early larval development of O. niloticus.

Copyright

Corresponding author

Address for correspondence: Elizete Rizzo. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Minas Gerais, Brasil. E-mails: ictio@icb.ufmg.br; elizeterizzo@gmail.com

References

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Alderdice, DF (1988) Osmotic and ionic regulation in teleost eggs and larvae. In Fish Physiology (eds Hoar, WS and Randall, DJ), pp. 163251, Academic Press.
Altinok, I and Grizzle, JM (2001) Effects of low salinities on Flavobacterium columnare infection of euryhaline and freshwater stenohaline fish. J Fish Dis 24, 361–7.
Betancor, MB, Caballero, MJ, Benítez-Santana, T, Saleh, R, Atalah, E and Izquierdo, M (2013) Oxidative status and histological changes in sea bass larvae muscle in response to high dietary content of docosahexanoic acid DHA. J Fish Dis 36, 453–65.
Beux, LF and Zaniboni-Filho, E (2007) Survival and the growth of pintado (Pseudoplatystoma corruscans) post-larvae on different salinities. Braz Arch Biol Tech 5, 821–9.
Bhujel, RC (2000) A review of strategies for the management of Nile tilapia (Oreochromis niloticus) broodfish in seed production systems, especially hapa-based systems. Aquaculture 181, 3759.
Borode, AO, Balogun, AM and Omoyeni, BA (2002) Effect of salinity on embryonic development, hatchability, and growth of African catfish, Clarias gariepinus, eggs and larvae. J Appl Aquacult 12, 8993.
Brodeur, JC, Peck, LS and Johnston, IA (2002) Feeding increases MyoD and PCNA expression in myogenic progenitor cells of Notothenia coriiceps . J Fish Biol 60, 1475–85.
Food and Agriculture Organization of the United Nations (FAO) (2016) Cultured aquatic species information programme: Oreochromis niloticus (Linnaeus, 1758). Available at: http://www.fao.org/fishery/culturedspecies/Oreochromis_niloticus/en. Accessed on 9 December 2016.
Fridman, S, Bron, JE and Rana, KJ (2011) Ontogenetic changes in location and morphology of chloride cells during early life stages of the Nile tilapia Oreochromis niloticus adapted to fresh and brackish water. J Fish Biol 79, 597614.
Fridman, S, Bron, JE and Rana, KJ (2012a) Influence of salinity on embryogenesis, survival, growth and oxygen consumption in embryos and yolk-sac larvae of the Nile tilapia. Aquaculture 334–337, 182–90.
Fridman, S, Bron, JE and Rana, KJ (2012b) Ontogenetic changes in the osmoregulatory capacity of the Nile tilapia Oreochromis niloticus and implications for aquaculture. Aquaculture 356–357, 243–9.
Fridman, S, Rana, KJ and Bron, JE (2013) Confocal scanning laser microscopy 3D image analysis allows quantitative studies of functional state of ion regulatory cells in the Nile tilapia (Oreochromis niloticus) following salinity challenge. Microsc Res Tech 76, 412–8.
Fuchs, Y and Steller, H (2011) Programmed cell death in animal development and disease. Cell 147, 742–58.
Garcia, LO, Becker, AG, Copatti, CE and Baldisserotto, B (2007) Salt in the food and water as a supportive therapy for Ichthyophthirius multifilii infestation on silver catfish, Rhamdia quelen, fingerlings. J World Aquacult Soc 38, 111.
Hiroi, J, Kaneko, T, Uchida, K, Hasegawa, S and Tanaka, M (1998) Immunolocalization of vacuolar-type H+-ATPase in the yolk-sac membrane of Tilapia (Oreochromis mossambicus) larvae. Zool Sci 16, 447–53.
Hirose, S, Kaneko, T, Naito, N and Takei, Y (2003) Molecular biology of major components of chloride cells. Comp Bioch Physiol B 136, 593620.
Jenkins, VK, Timmons, AK and McCall, K (2013) Diversity of cell death pathways: insight from the fly ovary. Trends Cell Biol. 332, 159–70.
Johnston, IA (1999) Muscle development and growth: potential implications for flesh quality in fishes. Aquaculture 177, 99115.
Johnston, IA (2006) Environmental and plasticity of myogenesis in teleost fish. J Exp Biol 209, 2249–64
Jomori, RK, Luz, RK and Portella, MC (2012) Effect of salinity on larval rearing of pacu, Piaractus mesopotamicus, a freshwater species. J World Aquacult Soc 43, 423–32.
Jun, Q, Pao, X, Haizhen, W, Ruiwei, L and Hui, W (2012) Combined effect of temperature, salinity and density on the growth and feed utilization of Nile tilapia juveniles (Oreochromis niloticus). Aquacult Res 43, 1344–56.
Kelman, Z (1997) PCNA: structure, functions and interactions. Oncogene 14, 629–40.
Likongue, JS, Stecko, TD, Stauffer, JR Jr and Carline, RF (1996) Combined effects of water temperature and salinity on growth and feed utilization of juvenile Nile tilapia Oreochromis niloticus (Linnaeus). Aquaculture 146, 3746.
Little, DC and Hulata, G (2000) Strategies for tilapia seed production. In Tilapia: Biology and Exploitation (eds Beveridge, MCM and McAndrew, BJ), pp 226326, Kluwer Academic Publisher.
Loppion, G, Crespel, A, Martinez, AS, Auvray, P and Sourdaine, P (2008) Study of the potential spermatogonial stem cell compartment in dogfish testis, Scyliorhinus canicula L. Cell Tissue Res 332, 533–42.
Martins, YS, Melo, RMC, Campo-Junior, PHA, Santos, JCE, Luz, RK, Rizzo, E and Bazzoli, N (2014) Salinity and temperature variations reflecting on cellular PCNA, IGF-I and II expressions, body growth and muscle cellularity of a freshwater fish larvae. Gen Comp Endocr 202, 50–8.
Meier, P, Finch, A and Evan, G (2000) Apoptosis in development. Nature 47, 796801.
Melo, RMC, Martins, YS, Luz, RK, Rizzo, E and Bazzoli, N (2015) PCNA and apoptosis during post-spawning ovarian remodeling in the teleost Oreochromis niloticus . Tissue Cell 47, 541–9.
Rowlerson, A, Radaelli, G, Mascarello, F and Veggetti, A (1997) Regeneration of skeletal muscle in two teleost fish: Sparus aurata and Brachydanio rerio . Cell Tissue Res 289, 311–22.
Schultz, E and McCormick, KM (1994) Skeletal muscle satellite cells. Rev Physiol Biochem Pharmacol 123, 213–57
Shiraishi, K, Kaneko, T, Hasegawa, S and Hirano, T (1997) Development of multicellular complexes of chloride cells in the yolk-sac membrane of tilapia (Oreochromis mossambicus) embryos and larvae in seawater. Cell Tissue Res 288, 583–90.
Souza-Bastos, LR and Freire, CA (2009) The handling of salt by the neotropical cultured freshwater catfish Rhamdia quelen . Aquaculture 289, 167–74.
Suresh, AV and Lin, CK (1992) Tilapia culture in saline water: a review. Aquaculture 106, 201–26.
Takle, H and Andersen, Ø (2007) Caspases and apoptosis in fish. J Fish Biol 71, 326–49.
Thomé, RG, Domingos, FFT, Santos, HB, Martinelli, PM, Sato, Y, Rizzo, E and Bazzoli, N (2012) Apoptosis, cell proliferation and vitellogenesis during the folliculogenesis and follicular growth in teleost fish. Tissue Cell 44, 5462.
Villegas, CT (1990) Evaluation of the salinity tolerance of Oreochromis mossambicus, O. niloticus and their F1 hybrids. Aquaculture 85, 281–92.
Wang, Ji-Q, Lui, H, Po, H and Fan, L (1996) Influence of salinity on food consumption, growth and energy conversion efficiency of common carp (Cyprinus carpio) fingerlings. Aquaculture 148, 115–24.
Watanabe, WO, Kuo, CM and Huang, MC (1985) The ontogeny of salinity tolerance in the tilapias Oreochromis aureus, O. niloticus and O. mossambicus × O. niloticus hybrid, spawned and reared in freshwater. Aquaculture 47, 353–67.
Zadunaisky, JA (1996) Chloride cells and osmoregulation. Kidney Int 49, 1563–7.

Keywords

Low salinity negatively affects early larval development of Nile tilapia, Oreochromis niloticus: insights from skeletal muscle and molecular biomarkers

  • Luis Henrique Melo (a1), Yuri Simões Martins (a1), Rafael Magno Costa Melo (a1), Paula Suzanna Prado (a1), Ronald Kennedy Luz (a2), Nilo Bazzoli (a1) (a3) and Elizete Rizzo (a1)...

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