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Lipopolysaccharide-induced modulation in the expression of progesterone receptor and estradiol receptor leads to early pregnancy loss in mouse

Published online by Cambridge University Press:  19 July 2012

Varkha Agrawal ,
Mukesh Kumar Jaiswal  and
Yogesh Kumar Jaiswal
Varkha Agrawal
Affiliation:
Molecular Biology and Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior-474 011, India. Obstetrics and Gynecology, North Shore University Health System, Evanston, IL 60201, USA.
Mukesh Kumar Jaiswal
Affiliation:
Molecular Biology and Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior-474 011, India. Clinical Immunology Laboratory, Rosalind Franklin University of Medical and Science, 3333 Green Bay Road, North Chicago, IL 60064, USA.
Yogesh Kumar Jaiswal*
Affiliation:
Molecular Biology and Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior-474 011(M.P.), India.
*
All correspondence to: Y.K. Jaiswal. Molecular Biology and Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior-474 011(M.P.), India. e-mail: jaiswalmbri@gmail.com
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Summary

The objective of the present study was to investigate the effect of Gram-negative bacteria infection on ovarian steroid receptors, i.e. progesterone receptor (PR) and estradiol receptor (ER) during preimplantation days of pregnancy. A well established mouse model of Gram-negative bacteria infection was used to test this objective. Mice were treated with normal saline or lipopolysaccharide (LPS) on day 0.5 of pregnancy and used to collect embryos and uterine horns on day 1.5 to day 4.42 preimplantation day of pregnancy. Total RNA was extracted and reverse-transcription polymerase chain reaction (PCR) was performed to check the expression of PR and ER genes. The mRNA expression of PR and ER was altered in embryos and uterus of LPS-treated animals during preimplantation days of pregnancy studied. These results suggest that PR and ER play an important role in Gram-negative bacteria infection and induced implantation failure in mouse.

Keywords

17β-EstradiolEstrogen receptorLipopolysaccharideProgesteroneProgesterone receptor
Type
Research Article
Information
Zygote , Volume 21 , Issue 4 , November 2013 , pp. 337 - 344
Copyright
Copyright © Cambridge University Press 2012 

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