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Leukemia inhibitory factor stimulates the transition of primordial to primary follicle and supports the goat primordial follicle viability in vitro

Published online by Cambridge University Press:  18 March 2011

Janduí Escarião da Nóbrega Jr ,
Paulo Bayard Dias Gonçalves ,
Roberta Nogueira Chaves ,
Deborah de Melo Magalhães ,
Rafael Rossetto ,
Isabel Bezerra Lima-Verde ,
Gabriel Ribas Pereira ,
Cláudio Cabral Campello ,
José Ricardo Figueiredo  and
João Francisco Coelho de Oliveira
Janduí Escarião da Nóbrega Jr
Affiliation:
Laboratory of Biotechnology and Animal Reproduction – BioRep, Federal University of Santa Maria, CEP 97105–900, Santa Maria, RS, Brazil.
Paulo Bayard Dias Gonçalves
Affiliation:
Laboratory of Biotechnology and Animal Reproduction – BioRep, Federal University of Santa Maria, CEP 97105–900, Santa Maria, RS, Brazil.
Roberta Nogueira Chaves
Affiliation:
Laboratory of Oocytes and Preantral Follicle Manipulation – MOIFOPA, State University of Ceará, CEP 60740–903, Fortaleza, CE, Brazil.
Deborah de Melo Magalhães
Affiliation:
Laboratory of Oocytes and Preantral Follicle Manipulation – MOIFOPA, State University of Ceará, CEP 60740–903, Fortaleza, CE, Brazil.
Rafael Rossetto
Affiliation:
Laboratory of Oocytes and Preantral Follicle Manipulation – MOIFOPA, State University of Ceará, CEP 60740–903, Fortaleza, CE, Brazil.
Isabel Bezerra Lima-Verde
Affiliation:
Laboratory of Oocytes and Preantral Follicle Manipulation – MOIFOPA, State University of Ceará, CEP 60740–903, Fortaleza, CE, Brazil.
Gabriel Ribas Pereira
Affiliation:
Laboratory of Biotechnology and Animal Reproduction – BioRep, Federal University of Santa Maria, CEP 97105–900, Santa Maria, RS, Brazil.
Cláudio Cabral Campello
Affiliation:
Laboratory of Oocytes and Preantral Follicle Manipulation – MOIFOPA, State University of Ceará, CEP 60740–903, Fortaleza, CE, Brazil.
José Ricardo Figueiredo
Affiliation:
Laboratory of Oocytes and Preantral Follicle Manipulation – MOIFOPA, State University of Ceará, CEP 60740–903, Fortaleza, CE, Brazil.
João Francisco Coelho de Oliveira*
Affiliation:
Laboratory of Biotechnology and Animal Reproduction, Av. Roraima #1000, CEP 97105–900, Santa Maria, RS, Brazil.
*
All correspondence to: João Francisco Coelho de Oliveira. Laboratory of Biotechnology and Animal Reproduction, Av. Roraima #1000, CEP 97105–900, Santa Maria, RS, Brazil. Tel: +55 55 3220 8752. Fax: +55 55 3220 8484. e-mail: jf@biorep.ufsm.br
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Summary

The aim of this study was to evaluate the effect of leukemia inhibitory factor (LIF) on the activation and survival of preantral follicles cultured in vitro enclosed in ovarian fragments (in situ). Goat ovarian cortex was divided into fragments to be used in this study. One fragment was immediately fixed (fresh control – FC) and the remaining fragments were cultured in supplemented minimum essential medium (MEM) without (cultured control – CC) or with different concentrations of LIF (1, 10, 50, 100 or 200 ng/ml) for 1 or 7 days, at 39°C in air with 5% CO2. Fresh control, CC and treated ovarian fragments were processed for histological and fluorescence analysis. The percentage of histological normal preantral follicles cultured for 7 days with 1 ng/ml (49.3%), 10 ng/ml (58.6%) and 50 ng/ml (58%) of LIF was higher than in the CC (32.6%; p < 0.05). After 7 days of culture, the percentage of primordial follicles in situ cultured with LIF decreased and primary follicles increased in all LIF concentrations compared with FC and CC (p < 0.05). In conclusion, LIF induced primordial follicle activation and supported preantral follicle viability of goat ovarian tissues cultured for 7 days.

Keywords

GoatLeukemia inhibitory factorOvaryPreantral folliclesPrimordial follicle activation
Type
Research Article
Information
Zygote , Volume 20 , Issue 1 , February 2012 , pp. 73 - 78
Copyright
Copyright © Cambridge University Press 2011

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