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Leukemia inhibitory factor stimulates the transition of primordial to primary follicle and supports the goat primordial follicle viability in vitro

  • Janduí Escarião da Nóbrega (a1), Paulo Bayard Dias Gonçalves (a1), Roberta Nogueira Chaves (a2), Deborah de Melo Magalhães (a2), Rafael Rossetto (a2), Isabel Bezerra Lima-Verde (a2), Gabriel Ribas Pereira (a1), Cláudio Cabral Campello (a2), José Ricardo Figueiredo (a2) and João Francisco Coelho de Oliveira (a3)...

Summary

The aim of this study was to evaluate the effect of leukemia inhibitory factor (LIF) on the activation and survival of preantral follicles cultured in vitro enclosed in ovarian fragments (in situ). Goat ovarian cortex was divided into fragments to be used in this study. One fragment was immediately fixed (fresh control – FC) and the remaining fragments were cultured in supplemented minimum essential medium (MEM) without (cultured control – CC) or with different concentrations of LIF (1, 10, 50, 100 or 200 ng/ml) for 1 or 7 days, at 39°C in air with 5% CO2. Fresh control, CC and treated ovarian fragments were processed for histological and fluorescence analysis. The percentage of histological normal preantral follicles cultured for 7 days with 1 ng/ml (49.3%), 10 ng/ml (58.6%) and 50 ng/ml (58%) of LIF was higher than in the CC (32.6%; p < 0.05). After 7 days of culture, the percentage of primordial follicles in situ cultured with LIF decreased and primary follicles increased in all LIF concentrations compared with FC and CC (p < 0.05). In conclusion, LIF induced primordial follicle activation and supported preantral follicle viability of goat ovarian tissues cultured for 7 days.

Copyright

Corresponding author

All correspondence to: João Francisco Coelho de Oliveira. Laboratory of Biotechnology and Animal Reproduction, Av. Roraima #1000, CEP 97105–900, Santa Maria, RS, Brazil. Tel: +55 55 3220 8752. Fax: +55 55 3220 8484. e-mail: jf@biorep.ufsm.br

References

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Leukemia inhibitory factor stimulates the transition of primordial to primary follicle and supports the goat primordial follicle viability in vitro

  • Janduí Escarião da Nóbrega (a1), Paulo Bayard Dias Gonçalves (a1), Roberta Nogueira Chaves (a2), Deborah de Melo Magalhães (a2), Rafael Rossetto (a2), Isabel Bezerra Lima-Verde (a2), Gabriel Ribas Pereira (a1), Cláudio Cabral Campello (a2), José Ricardo Figueiredo (a2) and João Francisco Coelho de Oliveira (a3)...

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