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Kdm6a overexpression improves the development of cloned mouse embryos

  • Guang-yu Bai (a1), Si-hang Song (a1), Yu-wei Zhang (a1), Xiang Huang (a1), Xing-wei Huang (a1), Rui-zhen Sun (a1) and Lei Lei (a2)...

Summary

Somatic cell nuclear transfer (SCNT) is an important technique for life science research. However, most SCNT embryos fail to develop to term due to undefined reprogramming defects. Here, we show that abnormal Xi occurs in somatic cell NT blastocysts, whereas in female blastocysts derived from cumulus cell nuclear transfer, both X chromosomes were inactive. H3K27me3 removal by Kdm6a mRNA overexpression could significantly improve preimplantation development of NT embryos, and even reached a 70.2% blastocyst rate of cleaved embryos compared with the 38.5% rate of the control. H3K27me3 levels were significantly reduced in blastomeres from cloned blastocysts after overexpression of Kdm6a. qPCR indicated that rDNA transcription increased in both NT embryos and 293T cells after overexpression of Kdm6a. Our findings demonstrate that overexpression of Kdm6a improved the development of cloned mouse embryos by reducing H3K27me3 and increasing rDNA transcription.

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Corresponding author

All correspondence to: Lei Lei. Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China. Tel: +086 451 8667451. Fax: +086 451 87503326. E-mail: Leiys2002@yahoo.com

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These authors contributed equally to this work.

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