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Isolation of viable egg cells of rape (Brassica napus L.)

Published online by Cambridge University Press:  26 September 2008

Norio Katoh ,
Horst Lörz  and
Erhard Kranz
Norio Katoh
Affiliation:
Japan Tobaco Inc., Oyama, Japan, and Centre for Applied Plant Molecular Biology, Hamburg, Germany
Horst Lörz
Affiliation:
Japan Tobaco Inc., Oyama, Japan, and Centre for Applied Plant Molecular Biology, Hamburg, Germany
Erhard Kranz*
Affiliation:
Japan Tobaco Inc., Oyama, Japan, and Centre for Applied Plant Molecular Biology, Hamburg, Germany
*
Dr.Erhard Kranz, Centre for Applied Plant Molecular Biology (AMP II), University of Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany.
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Summary

The isolation of viable egg cells of rape (Brassica napus L.) has been achieved from microdissected ovules. The non-gametic cells of the embryo sac, synergids and central cells have also been isolated. Their morphology corresponded to that of these cells in situ, making a discrimination from isolated sporophytic cells possible. Two hours after isolation the egg cells were still viable. Viable egg cells have been reproducibly isolated with afrequency of 25% per dissected ovule.

Keywords

Egg cell isolationEmbryo sacBrassica
Type
Article
Information
Zygote , Volume 5 , Issue 1 , February 1997 , pp. 31 - 33
Copyright
Copyright © Cambridge University Press 1997

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