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In vitro fertilisation of mouse oocytes reconstructed by transfer of metaphase II chromosomes results in live births

  • Min-Kang Wang (a1) (a2), Da-Yuan Chen (a1), Ji-Long Lui (a1), Guang-Peng Li (a1) and Qing-Yuan Sun (a1)...

Abstract

The interaction between nucleus and cytoplasm can be explored through nuclear transfer. We describe here another tool to investigate this interaction: MII meiotic apparatus transfer (MAT) between mouse oocytes. In this study, the MII oocyte meiotic apparatus or spindle from C57BL/6 mice, a black strain, was transferred into an enucleated metaphase oocyte from Kunming mouse, a white strain. The results showed that the enucleation rate by treating oocytes with 3% sucrose was 100%, but the electrofusion efficiency was very low, with only 17.6% of reconstructed karyoplast-recipient cytoplasm pairs fused. When the fused oocytes were exposed to spermatozoa from C57BL/6 mice, 9 of 11 (82%) were fertilised. Eight reconstructed embryos at 1- to 4-cell stages were transferred into the oviducts of two synchronously pregnant Kunming strain fosters and one delivered two normal C57BL/6 offspring. This study indicates that MII meiotic apparatus or spindle sustains normal structure and function after micromanipulation and electrofusion. MAT provides a model for further research on the application of this technique to assisted human reproduction.

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Corresponding author

All correspondence to: Da-Yuan Chen, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China. Tel: (8610) 62560528, 62562793. Fax: (8610) 62565689. e-mail: chendy@panda.ioz.ac.cn

Keywords

In vitro fertilisation of mouse oocytes reconstructed by transfer of metaphase II chromosomes results in live births

  • Min-Kang Wang (a1) (a2), Da-Yuan Chen (a1), Ji-Long Lui (a1), Guang-Peng Li (a1) and Qing-Yuan Sun (a1)...

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