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Impact of oocyte-secreted factors on its developmental competence in buffalo

  • Swati Gupta (a1), Sriti Pandey (a1), Mehtab S. Parmar (a1), Anjali Somal (a1), Avishek Paul (a1), Bibhudatta S. K. Panda (a1), Irfan A. Bhat (a1), Indu Baiju (a1), Mukesh K. Bharti (a1), G. Saikumar (a1), Mihir Sarkar (a1), Vikash Chandra (a1) and G. Taru Sharma (a1)...


Oocyte-secreted factors (OSFs) play an important role in the acquisition of oocyte developmental competence through bidirectional cross-talk between oocyte and cumulus cells via gap junctions. Thus, the present study was designed to investigate the effect of two OSFs, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), on the developmental competence of buffalo oocytes derived from two different follicle sizes. Cumulus–oocyte complexes (COCs) from large follicles (LF, >6 mm) or small follicles (SF, <6 mm) were collected and matured in vitro either in the presence of GDF9 or BMP15, or both, or with the denuded oocytes (DOs) as a source of native OSFs. Cleavage and blastocyst rates were significantly (P < 0.05) higher in LF-derived than SF-derived oocytes. Cleavage and blastocyst rates were significantly higher (P < 0.05) in the DOs and the combination groups compared with the control, GDF9 alone and BMP15 alone groups, both in LF-derived and SF-derived oocytes, although the cleavage and blastocyst rates did not differ significantly (P > 0.05) between DOs and combination groups. Relative mRNA analysis revealed significantly higher (P > 0.05) expression of the cumulus cell marker genes EGFR, HAS2, and CD44 in LF-derived than SF-derived oocyte; the expression of these markers was significantly higher (P > 0.05) in DOs and combination groups, irrespective of the follicle size. These results suggested that LF-derived oocytes have a higher developmental competence than SF-derived oocytes and that supplementation of GDF9 and BMP15 modulates the developmental competence of buffalo oocytes by increasing the relative abundance of cumulus-enabling factors and thereby increasing cleavage and the quality of blastocyst production.


Corresponding author

All correspondence to: G. Taru Sharma. Reproductive Physiology Laboratory, Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, UP, India. Tel:/Fax:: +91 0581 2301327/ +91 9412 603840. E-mail:


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Impact of oocyte-secreted factors on its developmental competence in buffalo

  • Swati Gupta (a1), Sriti Pandey (a1), Mehtab S. Parmar (a1), Anjali Somal (a1), Avishek Paul (a1), Bibhudatta S. K. Panda (a1), Irfan A. Bhat (a1), Indu Baiju (a1), Mukesh K. Bharti (a1), G. Saikumar (a1), Mihir Sarkar (a1), Vikash Chandra (a1) and G. Taru Sharma (a1)...


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